HAMAP rule MF_00320
General rule information
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| Accession | MF_00320 |
| Dates | 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 31) |
| Name | RNApol_arch_Rpo3 |
| Scope(s) |
Archaea |
| Template(s) | P95989 (RPO3_SACS2); B8YB56 (RPO3_SACSH); P39471 (RPO3_SULAC); Q5JJF4 (RPO3_THEKO); [ Recover all ] |
| Triggered by |
HAMAP; MF_00320 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RPO3 |
| Protein name | RecName: Full=DNA-directed RNA polymerase subunit Rpo3; EC=2.7.7.6; AltName: Full=DNA-directed RNA polymerase subunit D; |
| Gene name | Name=rpo3; Synonyms=rpoD; |
Comments
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| FUNCTION | DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. |
| CATALYTIC ACTIVITY | Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.6; |
| case <FTGroup:1> | |
| COFACTOR | Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137; Note=Binds 1 [3Fe-4S] cluster.; |
| end case | |
| SUBUNIT | Part of the RNA polymerase complex. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family. |
Keywords
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| Cytoplasm | |
| DNA-directed RNA polymerase | |
| case <FTGroup:1> | |
| 3Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| end case | |
| Nucleotidyltransferase | |
| Transcription | |
| Transferase | |
Gene Ontology
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| GO:0005737; Cellular component:cytoplasm | |
| GO:0003677; Molecular function:DNA binding | |
| GO:0003899; Molecular function:DNA-directed 5'-3' RNA polymerase activity | |
| case <FTGroup:1> | |
| GO:0051536; Molecular function:iron-sulfur cluster binding | |
| end case | |
| GO:0006351; Biological process:DNA-templated transcription | |
Cross-references
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| PROSITE | PS00446; RNA_POL_D_30KD; 1; |
| PROSITE | PS00198; 4FE4S_FER_1; 0-1; |
| PROSITE | PS51379; 4FE4S_FER_2; 0-1; |
| Pfam | PF01000; RNA_pol_A_bac; 1; |
| Pfam | PF01193; RNA_pol_L; 1; |
Features
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| From: RPO3_SACS2 (P95989) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 203 | 203 | /ligand="[3Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:21137" |
C | 1 | |||||||
| BINDING | 206 | 206 | /ligand="[3Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:21137" |
C | 1 | |||||||
| BINDING | 209 | 209 | /ligand="[3Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:21137" |
C | 1 | |||||||
Additional information
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| Size range | 191-305 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |