HAMAP rule MF_00331
General rule information
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Accession | MF_00331 |
Dates | 1-JUN-2001 (Created) 17-JAN-2023 (Last updated, Version 42) |
Name | Cys_desulf_IscS |
Scope | Bacteria
Archaea |
Templates | P0A6B7 (ISCS_ECOLI); O31269 (ISCS_AZOVI); O29689 (ISCS2_ARCFU); P0A6B9 (ISCS_ECO57): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Function | Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur and selenium atoms from cysteine and selenocysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. Also functions as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate. |
else
Function | Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. |
end case
Catalytic activity | RHEA:43892: [sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine
EC 2.8.1.7 |
Cofactor | pyridoxal 5'-phosphate |
Pathway | Cofactor biosynthesis; iron-sulfur cluster biosynthesis. |
Subunit | Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors. |
Subcellular location | Cytoplasm. |
case <OC:Archaea>
Miscellaneous | In Archaea the pyridoxal phosphate cofactor is not covalently bound to Lys but ligated by other amino acids. |
end case
Similarity | Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily. |
Keywords
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case <FTTag:IronSulfur>
end case
Gene Ontology
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GO:0005737; Cellular component: cytoplasm.
GO:0030170; Molecular function: pyridoxal phosphate binding.
GO:0031071; Molecular function: cysteine desulfurase activity.
GO:0030170; Molecular function: pyridoxal phosphate binding.
GO:0031071; Molecular function: cysteine desulfurase activity.
case <FTTag:IronSulfur>
GO:0051537; Molecular function: 2 iron, 2 sulfur cluster binding.
end case
GO:0044571; Biological process: [2Fe-2S] cluster assembly.
Cross-references
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Pfam | PF00266; Aminotran_5; 1; |
PIRSF | PIRSF005572; NifS; 1; |
TIGRFAMs | TIGR02006; IscS; 1; |
TIGR03402; FeS_nifS; 1; | |
TIGR03403; nifS_epsilon; 1; | |
PROSITE | PS00595; AA_TRANSFER_CLASS_5; 1; |
Features
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From: ISCS_ECO57 (P0A6B9) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 75 | 76 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326 | [AG]-[TS] | ||||||||
BINDING | 203 | 205 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326 | [TS]-x-H | ||||||||
ACT_SITE | 328 | 328 | Cysteine persulfide intermediate | C | ||||||||
BINDING | 328 | 328 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135" /ligand_note="ligand shared with IscU" /note="via persulfide group | IronSulfur | C | |||||||
BINDING | 155 | 155 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326 | N | ||||||||
BINDING | 183 | 183 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326 | Q | ||||||||
BINDING | 243 | 243 | /ligand="pyridoxal 5'-phosphate" /ligand_id="ChEBI:CHEBI:597326 | [TS] |
case <OC:Bacteria>
end case
Additional information
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Size range | 375-410 amino acids |
Related rules | None |
Fusion | None |
Comments | Divergent HALH5 and BACSU; sequences not included in alignment |