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Annotation rule MF_00331 |
General rule information
[?]
Accession |
MF_00331 |
Dates |
1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 37) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=Cysteine desulfurase IscS;
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EC 2.8.1.7;
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case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Function |
Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur and selenium atoms from cysteine and selenocysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. Also functions as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate. |
else
Function |
Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. |
end case
Catalytic activity |
RHEA:43892: [sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine
EC 2.8.1.7
|
Cofactor |
pyridoxal 5'-phosphate |
Pathway |
Cofactor biosynthesis; iron-sulfur cluster biosynthesis. |
Subunit |
Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors. |
Subcellular location |
Cytoplasm. |
case <OC:Archaea>
Miscellaneous |
In Archaea the pyridoxal phosphate cofactor is not covalently bound to Lys but ligated by other amino acids. |
end case
Similarity |
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily. |
case <FTTag:IronSulfur>
end case
GO:0005737; Cellular component: cytoplasm.
GO:0030170; Molecular function: pyridoxal phosphate binding.
GO:0031071; Molecular function: cysteine desulfurase activity.
case <FTTag:IronSulfur>
GO:0051537; Molecular function: 2 iron, 2 sulfur cluster binding.
end case
GO:0044571; Biological process: [2Fe-2S] cluster assembly.
From: ISCS_ECO57 (P0A6B9) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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REGION
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75
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76
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Pyridoxal phosphate binding
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[AG]-[TS]
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REGION
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203
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205
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Pyridoxal phosphate binding
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[TS]-x-H
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ACT_SITE
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328
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328
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Cysteine persulfide intermediate
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C
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METAL
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328
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328
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Iron-sulfur (2Fe-2S); via persulfide group; shared with IscU
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IronSulfur
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C
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BINDING
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155
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155
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Pyridoxal phosphate
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N
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BINDING
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183
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183
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Pyridoxal phosphate
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Q
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BINDING
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243
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243
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Pyridoxal phosphate
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[TS]
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case <OC:Bacteria>
MOD_RES
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206
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206
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N6-(pyridoxal phosphate)lysine
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K
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end case
Additional information
[?]
Size range |
375-410 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
Divergent BACHD and BACSU; sequences not included in alignment |