HAMAP rule MF_00339
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00339 |
| Accession | MF_00339 |
| Dates | 1-JUN-2001 (Created)
2-SEP-2024 (Last updated, Version 46) |
| Name | Phosphofructokinase_I_B1 |
| Scope(s) |
Bacteria |
| Template(s) | P0A796 (PFKA_ECOLI); P00512 (PFKA_GEOSE); P0DOB6 (PFKA1_LACLL); Q9WY52 (PFKA_THEMA); P21777 (PFKA1_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00339 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PFKA |
| Protein name | RecName: Full=ATP-dependent 6-phosphofructokinase; Short=ATP-PFK; Short=Phosphofructokinase; EC=2.7.1.11; AltName: Full=Phosphohexokinase; |
| Gene name | Name=pfkA; |
Comments
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| FUNCTION | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. |
| CATALYTIC ACTIVITY | Reaction=beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6- bisphosphate + ADP + H(+); Xref=Rhea:RHEA:16109, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32966, ChEBI:CHEBI:57634, ChEBI:CHEBI:456216; EC=2.7.1.11; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| ACTIVITY REGULATION | Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate. |
| PATHWAY | Carbohydrate degradation; glycolysis; D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4. |
| SUBUNIT | Homotetramer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade 'B1' sub- subfamily. |
Keywords
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| Allosteric enzyme |
| Cytoplasm |
| Kinase |
| Transferase |
| Glycolysis |
| ATP-binding |
| Nucleotide-binding |
| Magnesium |
| Metal-binding |
Gene Ontology
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| GO:0003872; Molecular function:6-phosphofructokinase activity |
| GO:0006096; Biological process:glycolytic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00365; PFK; 1; |
| PIRSF | PIRSF000532; ATP_PFK_prok; 1; |
| PRINTS | PR00476; PHFRCTKINASE; 1; |
| NCBIfam | TIGR02482; PFKA_ATP; 1; |
| PROSITE | PS00433; PHOSPHOFRUCTOKINASE; 1; |
Features
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| From: PFKA_ECOLI (P0A796) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 73 | 74 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R-x | ||||||||
| BINDING | 103 | 106 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-[DEN]-G-[ST] | ||||||||
| BINDING | 22 | 26 | /ligand="ADP" /ligand_id="ChEBI:CHEBI:456216" /ligand_note="allosteric activator; ligand shared between dimeric partners" |
R-x(3)-[RK] | ||||||||
| BINDING | 55 | 60 | /ligand="ADP" /ligand_id="ChEBI:CHEBI:456216" /ligand_note="allosteric activator; ligand shared between dimeric partners" |
R-Y-x(2)-S-[DE] | ||||||||
| BINDING | 126 | 128 | /ligand="substrate" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
[TS]-x-D | ||||||||
| BINDING | 170 | 172 | /ligand="substrate" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
M-G-[RHN] | ||||||||
| BINDING | 186 | 188 | /ligand="ADP" /ligand_id="ChEBI:CHEBI:456216" /ligand_note="allosteric activator; ligand shared between dimeric partners" /note="in other chain" |
G-x-[ED] | ||||||||
| BINDING | 214 | 216 | /ligand="ADP" /ligand_id="ChEBI:CHEBI:456216" /ligand_note="allosteric activator; ligand shared between dimeric partners" /note="in other chain" |
K-x(2) | ||||||||
| BINDING | 250 | 253 | /ligand="substrate" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
[HY]-x(2)-R | ||||||||
| ACT_SITE | 128 | 128 | /note="Proton acceptor" | D | ||||||||
| BINDING | 104 | 104 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="catalytic" |
[DEN] | ||||||||
| BINDING | 12 | 12 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| BINDING | 155 | 155 | /ligand="ADP" /ligand_id="ChEBI:CHEBI:456216" /ligand_note="allosteric activator; ligand shared between dimeric partners" /note="in other chain" |
[RK] | ||||||||
| BINDING | 163 | 163 | /ligand="substrate" /ligand_note="ligand shared between dimeric partners" |
R | ||||||||
| BINDING | 212 | 212 | /ligand="ADP" /ligand_id="ChEBI:CHEBI:456216" /ligand_note="allosteric activator; ligand shared between dimeric partners" /note="in other chain" |
[RK] | ||||||||
| BINDING | 223 | 223 | /ligand="substrate" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
E | ||||||||
| BINDING | 244 | 244 | /ligand="substrate" /ligand_note="ligand shared between dimeric partners" |
[RK] | ||||||||
Additional information
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| Size range | 319-361 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511). |