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HAMAP rule MF_00355

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General rule information [?]

Accession MF_00355
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 48)
Name ChlL_BchL
Scope
Bacteria
Plastid
Templates Q9RFD6 (BCHL_CERS4); P0CY53 (BCHL_RHOCA); D5ANS3 (BCHL_RHOCB): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Bacteria> and not <OC:Cyanobacteriota>
Identifier
BCHL
end case
case <OC:Cyanobacteriota> or <OG:Chloroplast>
Identifier
CHLL
end case
Protein name
RecName: Full=Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein;
Short=DPOR subunit L;
Short=LI-POR subunit L;
EC 1.3.7.7;
case <OC:Bacteria> and not <OC:Cyanobacteriota>
Gene name
bchL
end case
case <OC:Cyanobacteriota> or <OG:Chloroplast>
Gene name
chlL
end case

Comments [?]

Function Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The L component serves as a unique electron donor to the NB-component of the complex, and binds Mg-ATP.
Catalytic activity RHEA:28202: 2 ADP + chlorophyllide a + oxidized 2[4Fe-4S]-[ferredoxin] + 2 phosphate = 2 ATP + 2 H2O + protochlorophyllide a + reduced 2[4Fe-4S]-[ferredoxin]
EC 1.3.7.7
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster per dimer.
end case
case <OC:Bacteria> and not <OC:Cyanobacteriota>
Pathway Porphyrin-containing compound metabolism; bacteriochlorophyll biosynthesis (light-independent).
Subunit Homodimer. Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB.
end case
case <OC:Cyanobacteriota> or <OG:Chloroplast>
Pathway Porphyrin-containing compound metabolism; chlorophyll biosynthesis (light-independent).
Subunit Homodimer. Protochlorophyllide reductase is composed of three subunits; ChlL, ChlN and ChlB.
end case
case <OG:Chloroplast>
Subcellular location Plastid, chloroplast.
end case
Similarity Belongs to the NifH/BchL/ChlL family.

Keywords [?]

Oxidoreductase
Photosynthesis
Chlorophyll biosynthesis
case <OC:Bacteria> and not <OC:Cyanobacteriota>
Bacteriochlorophyll biosynthesis
end case
ATP-binding
Nucleotide-binding
Magnesium
Metal-binding
case <FTGroup:1>
Iron
Iron-sulfur
4Fe-4S
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0016636; Molecular function: oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor.
case <FTGroup:1>
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
end case
case <OC:Bacteria> and not <OC:Cyanobacteriota>
GO:0030494; Biological process: bacteriochlorophyll biosynthetic process.
end case
case <OC:Cyanobacteriota> or <OG:Chloroplast>
GO:0015995; Biological process: chlorophyll biosynthetic process.
end case
GO:0015979; Biological process: photosynthesis.
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

Pfam PF00142; Fer4_NifH; 1;
PIRSF PIRSF000363; Nitrogenase_iron; 1;
PRINTS PR00091; NITROGNASEII; 1;
NCBIfam TIGR01281; DPOR_bchL; 1;
PROSITE PS00746; NIFH_FRXC_1; 1;
PS00692; NIFH_FRXC_2; 1;
PS51026; NIFH_FRXC_3; 1;

Features [?]

From: BCHL_CERS4 (Q9RFD6)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     41     46       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-[IV]-G-K-S-T  
BINDING     211     212       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     N-R  
BINDING (Optional)     235     237       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     P-x-L  
BINDING     45     45       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     S  
BINDING     126     126       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="ligand shared between dimeric partners     C   1
BINDING     160     160       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="ligand shared between dimeric partners     C   1
BINDING     70     70       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     K  

Additional information [?]

Size range 245-331 amino acids
Related rules MF_00533 (NIFH)
Fusion None
Comments External expert: Yuichi Fujita, fujita@protein.osaka-u.ac.jp


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