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HAMAP rule MF_00377

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_00377
Accession MF_00377
Dates 1-JUN-2001 (Created)
8-NOV-2024 (Last updated, Version 35)
Name DnaA_bact
Scope(s) Bacteria
Template(s) P03004 (DNAA_ECOLI); P05648 (DNAA_BACSU); O66659 (DNAA_AQUAE); [ Recover all ]
Triggered by HAMAP; MF_00377 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DNAA
Protein name RecName: Full=Chromosomal replication initiator protein DnaA;
Gene name Name=dnaA;

Comments [?]

FUNCTIONPlays an essential role in the initiation and regulation of chromosomal replication. ATP-DnaA binds to the origin of replication (oriC) to initiate formation of the DNA replication initiation complex once per cell cycle. Binds the DnaA box (a 9 base pair repeat at the origin) and separates the double-stranded (ds)DNA. Forms a right-handed helical filament on oriC DNA; dsDNA binds to the exterior of the filament while single-stranded (ss)DNA is stabiized in the filament's interior. The ATP-DnaA-oriC complex binds and stabilizes one strand of the AT-rich DNA unwinding element (DUE), permitting loading of DNA polymerase. After initiation quickly degrades to an ADP-DnaA complex that is not apt for DNA replication. Binds acidic phospholipids.
SUBUNITOligomerizes as a right-handed, spiral filament on DNA at oriC.
SUBCELLULAR LOCATIONCytoplasm.
DOMAINDomain I is involved in oligomerization and binding regulators, domain II is flexibile and of varying length in different bacteria, domain III forms the AAA+ region, while domain IV binds dsDNA.
SIMILARITYBelongs to the DnaA family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0003688; Molecular function:DNA replication origin binding
GO:0006270; Biological process:DNA replication initiation
GO:0006275; Biological process:regulation of DNA replication
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00308; Bac_DnaA; 1;
Pfam PF08299; Bac_DnaA_C; 1;
PRINTS PR00051; DNAA; 1;
NCBIfam TIGR00362; DnaA; 1;
PROSITE PS01008; DNAA; 1;

Features [?]

From: DNAA_ECOLI (P03004)
Key From To Description Tag Condition FTGroup
REGION Nter 90 /note="Domain I, interacts with DnaA modulators"
REGION 91 130 /note="Domain II"
REGION 131 347 /note="Domain III, AAA+ region"
REGION 348 Cter /note="Domain IV, binds dsDNA"
From: DNAA_AQUAE (O66659)
Key From To Description Tag Condition FTGroup
BINDING 122 122 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[GSA]
BINDING 124 124 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[GS]
BINDING 125 125 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
K
BINDING 126 126 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[ST]

Additional information [?]

Size range 399-656 amino acids
Related rules None
Fusion Nter: None Cter: None



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