HAMAP rule MF_00377
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00377 |
| Accession | MF_00377 |
| Dates | 1-JUN-2001 (Created)
8-NOV-2024 (Last updated, Version 35) |
| Name | DnaA_bact |
| Scope(s) |
Bacteria |
| Template(s) | P03004 (DNAA_ECOLI); P05648 (DNAA_BACSU); O66659 (DNAA_AQUAE); [ Recover all ] |
| Triggered by |
HAMAP; MF_00377 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DNAA |
| Protein name | RecName: Full=Chromosomal replication initiator protein DnaA; |
| Gene name | Name=dnaA; |
Comments
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| FUNCTION | Plays an essential role in the initiation and regulation of chromosomal replication. ATP-DnaA binds to the origin of replication (oriC) to initiate formation of the DNA replication initiation complex once per cell cycle. Binds the DnaA box (a 9 base pair repeat at the origin) and separates the double-stranded (ds)DNA. Forms a right-handed helical filament on oriC DNA; dsDNA binds to the exterior of the filament while single-stranded (ss)DNA is stabiized in the filament's interior. The ATP-DnaA-oriC complex binds and stabilizes one strand of the AT-rich DNA unwinding element (DUE), permitting loading of DNA polymerase. After initiation quickly degrades to an ADP-DnaA complex that is not apt for DNA replication. Binds acidic phospholipids. |
| SUBUNIT | Oligomerizes as a right-handed, spiral filament on DNA at oriC. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| DOMAIN | Domain I is involved in oligomerization and binding regulators, domain II is flexibile and of varying length in different bacteria, domain III forms the AAA+ region, while domain IV binds dsDNA. |
| SIMILARITY | Belongs to the DnaA family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0003688; Molecular function:DNA replication origin binding |
| GO:0006270; Biological process:DNA replication initiation |
| GO:0006275; Biological process:regulation of DNA replication |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00308; Bac_DnaA; 1; |
| Pfam | PF08299; Bac_DnaA_C; 1; |
| PRINTS | PR00051; DNAA; 1; |
| NCBIfam | TIGR00362; DnaA; 1; |
| PROSITE | PS01008; DNAA; 1; |
Features
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| From: DNAA_ECOLI (P03004) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 90 | /note="Domain I, interacts with DnaA modulators" | |||||||||
| REGION | 91 | 130 | /note="Domain II" | |||||||||
| REGION | 131 | 347 | /note="Domain III, AAA+ region" | |||||||||
| REGION | 348 | Cter | /note="Domain IV, binds dsDNA" | |||||||||
| From: DNAA_AQUAE (O66659) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 122 | 122 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[GSA] | ||||||||
| BINDING | 124 | 124 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[GS] | ||||||||
| BINDING | 125 | 125 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
| BINDING | 126 | 126 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[ST] | ||||||||
Additional information
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| Size range | 399-656 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |