Home  |  Contact
Annotation rule MF_00379
Send feedback

General rule information [?]

Accession MF_00379
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 33)
Name GTPase_MnmE
Templates P25522 (MNME_ECOLI); Q9WYA4 (MNME_THEMA): [Recover all]
case <OC:Bacteria> or <OG:Chloroplast>
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Bacteria>
Protein name
RecName: Full=tRNA modification GTPase MnmE;
EC 3.6.-.-;
Gene name
mnmE, trmE
else case <OG:Chloroplast>
Protein name
RecName: Full=Probable tRNA modification GTPase MnmE;
EC 3.6.-.-;
Gene name
mnmE, thdF
end case

Comments [?]

Function Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.
Cofactor K(+)
Note: Binds 1 potassium ion per subunit.
case <OC:Bacteria>
Subunit Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
Subcellular location Cytoplasm.
else case <OG:Chloroplast>
Subcellular location Plastid, chloroplast.
end case
Similarity Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.

Keywords [?]

case <OC:Bacteria>
end case

Gene Ontology [?]

GO:0005525; Molecular function: GTP binding.
GO:0003924; Molecular function: GTPase activity.
GO:0006400; Biological process: tRNA modification.
case <OC:Bacteria>
GO:0005737; Cellular component: cytoplasm.
else case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

Pfam PF01926; MMR_HSR1; 1;
PF10396; TrmE_N; 1;
TIGRFAMs TIGR00450; MnmE_trmE_thdF; 1;
TIGR00231; Small_GTP; 1;

Features [?]

From: MNME_ECOLI (P25522)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     226     231       GTP     N-[VSAT]-G-K-S-[TSQR]  
NP_BIND     245     251       GTP     [TSMAD]-x(3)-G-[TSR]-T  
NP_BIND     270     273       GTP     D-[TS]-A-G  
NP_BIND (Optional)     335     338       GTP     N-K-A-D  
NP_BIND (Optional)     358     360       GTP     S-A-R  
METAL (Optional)     226     226       Potassium     N   1
METAL     230     230       Magnesium     S  
METAL (Optional)     245     245       Potassium; via carbonyl oxygen     [TS]   1
METAL (Optional)     247     247       Potassium; via carbonyl oxygen     [IVL]   1
METAL (Optional)     250     250       Potassium     [TS]   1
METAL     251     251       Magnesium     T  
BINDING     23     23       Formyltetrahydrofolate     [RK]   2
BINDING     80     80       Formyltetrahydrofolate     E   2
BINDING     120     120       Formyltetrahydrofolate     [RKVM]   2
BINDING     454     454       Formyltetrahydrofolate     K   2

Additional information [?]

Size range 419-550 amino acids
Related rules None
Fusion None