HAMAP rule MF_00379
General rule information
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| Accession | MF_00379 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 36) |
| Name | GTPase_MnmE |
| Scope(s) |
Bacteria Plastid |
| Template(s) | P25522 (MNME_ECOLI); Q9WYA4 (MNME_THEMA); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria> or <OG:Chloroplast>
HAMAP; MF_00379 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MNME |
| case <OC:Bacteria> | |
| Protein name | RecName: Full=tRNA modification GTPase MnmE; EC=3.6.-.-; |
| Gene name | Name=mnmE; Synonyms=trmE; |
| else case <OG:Chloroplast> | |
| Protein name | RecName: Full=Probable tRNA modification GTPase MnmE; EC=3.6.-.-; |
| Gene name | Name=mnmE; Synonyms=thdF; |
| end case | |
Comments
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| FUNCTION | Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34. |
| COFACTOR | Name=K(+); Xref=ChEBI:CHEBI:29103; Note=Binds 1 potassium ion per subunit.; |
| case <OC:Bacteria> | |
| SUBUNIT | Homodimer. Heterotetramer of two MnmE and two MnmG subunits. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| else case <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| end case | |
| SIMILARITY | Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family. |
Keywords
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| case <OC:Bacteria> | |
| Cytoplasm | |
| end case | |
| GTP-binding | |
| Hydrolase | |
| Magnesium | |
| Metal-binding | |
| Nucleotide-binding | |
| Potassium | |
| tRNA processing | |
Gene Ontology
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| GO:0005525; Molecular function:GTP binding | |
| GO:0003924; Molecular function:GTPase activity | |
| GO:0006400; Biological process:tRNA modification | |
| case <OCellular component:Bacteria> | |
| GO:0005737; Cellular component:cytoplasm | |
| else case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| end case | |
Cross-references
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| Pfam | PF01926; MMR_HSR1; 1; |
| Pfam | PF10396; TrmE_N; 1; |
| PRINTS | PR00326; GTP1OBG; 1; |
| NCBIfam | TIGR00450; MnmE_trmE_thdF; 1; |
| NCBIfam | TIGR00231; Small_GTP; 1; |
| PROSITE | PS51709; G_TRME; 1; |
Features
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| From: MNME_ECOLI (P25522) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 226 | 231 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
N-[VSAT]-G-K-S-[TSQR] | ||||||||
| BINDING | 245 | 251 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[TSMAD]-x(3)-G-[TSR]-T | ||||||||
| BINDING | 270 | 273 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D-[TS]-A-G | ||||||||
| BINDING | 335 | 338 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
N-K-A-D | ||||||||
| BINDING | 358 | 360 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
S-A-R | ||||||||
| BINDING | 226 | 226 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103" |
N | 1 | |||||||
| BINDING | 230 | 230 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
S | ||||||||
| BINDING | 245 | 245 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103" |
[TS] | 1 | |||||||
| BINDING | 247 | 247 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103" |
[IVL] | 1 | |||||||
| BINDING | 250 | 250 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103" |
[TS] | 1 | |||||||
| BINDING | 251 | 251 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
T | ||||||||
| BINDING | 23 | 23 | /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:57457" |
[RK] | 2 | |||||||
| BINDING | 80 | 80 | /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:57457" |
E | 2 | |||||||
| BINDING | 120 | 120 | /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:57457" |
[RKVM] | 2 | |||||||
| BINDING | 454 | 454 | /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:57457" |
K | 2 | |||||||
Additional information
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| Size range | 419-550 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |