Home  |  Contact
Annotation rule MF_00407
Send feedback

General rule information [?]

Accession MF_00407
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 29)
Name DNA_ligase
Scope
Bacteria
Archaea
Templates P56709 (DNLI_PYRFU); Q9HHC4 (DNLI_THEKO); Q980T8 (DNLI_SACS2); Q9P9K9 (DNLI_SACSH); A2BJX6 (DNLI_HYPBU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DNLI
case <OC:Archaea> and not <OC:Thermococcus>
Protein name
RecName: Full=DNA ligase;
EC 6.5.1.1;
AltName: Full=Polydeoxyribonucleotide synthase [ATP];
else case <OC:Thermococcus>
Protein name
RecName: Full=DNA ligase;
EC 6.5.1.6;
AltName: Full=Polydeoxyribonucleotide synthase [ATP/NAD(+)];
else case <OC:Bacteria>
Protein name
RecName: Full=Probable DNA ligase;
EC 6.5.1.1;
AltName: Full=Polydeoxyribonucleotide synthase [ATP];
end case
Gene name
lig

Comments [?]

Function DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
case <OC:Archaea> and not <OC:Thermococcus>
Catalytic activity Reaction=ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.; EC=6.5.1.1;
else case <OC:Thermococcus>
Catalytic activity Reaction=ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.; EC=6.5.1.6;
Reaction=NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.; EC=6.5.1.6;
else case <OC:Bacteria>
Catalytic activity Reaction=ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.; EC=6.5.1.1;
end case
Cofactor Mg(2+)
Similarity Belongs to the ATP-dependent DNA ligase family.

Keywords [?]

DNA damage
DNA repair
DNA replication
DNA recombination
Cell cycle
Cell division
Ligase
ATP-binding
Magnesium
Metal-binding
case <OC:Thermococcus>
NAD
end case
Nucleotide-binding

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0003910; Molecular function: DNA ligase (ATP) activity.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.
GO:0006260; Biological process: DNA replication.

Cross-references [?]

Pfam PF04679; DNA_ligase_A_C; 1;
PF01068; DNA_ligase_A_M; 1;
PF04675; DNA_ligase_A_N; 1;
TIGRFAMs TIGR00574; Dnl1; 1;
PROSITE PS00697; DNA_LIGASE_A1; 1;
PS00333; DNA_LIGASE_A2; 1;
PS50160; DNA_LIGASE_A3; 1;

Features [?]

From: DNLI_PYRFU (P56709)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     249     249       N6-AMP-lysine intermediate     K  
BINDING     247     247       ATP     [DE]  
BINDING     254     254       ATP     R  
BINDING     269     269       ATP     R  
BINDING     299     299       ATP     E  
BINDING     339     339       ATP     F  
BINDING     414     414       ATP     R  
BINDING     420     420       ATP     K  

Additional information [?]

Size range 503-618 amino acids
Related rules None
Fusion Nter: <Unknown>; Cter: None
Comments Shorter N-terminus in the first copy of lig in METBF. Some archaea use additional nucleotide cofactors (such as NAD(+), ADP or GTP) next to ATP.


View rule in raw text format (no links)