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Annotation rule MF_00407 |
General rule information
[?]
Accession |
MF_00407 |
Dates |
1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 29) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
case <OC:Archaea> and not <OC:Thermococcus>
Protein name |
RecName:
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Full=DNA ligase;
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EC 6.5.1.1;
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AltName:
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Full=Polydeoxyribonucleotide synthase [ATP];
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else case <OC:Thermococcus>
Protein name |
RecName:
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Full=DNA ligase;
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EC 6.5.1.6;
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AltName:
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Full=Polydeoxyribonucleotide synthase [ATP/NAD(+)];
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else case <OC:Bacteria>
Protein name |
RecName:
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Full=Probable DNA ligase;
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EC 6.5.1.1;
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AltName:
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Full=Polydeoxyribonucleotide synthase [ATP];
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end case
Function |
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. |
case <OC:Archaea> and not <OC:Thermococcus>
Catalytic activity |
Reaction=ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.; EC=6.5.1.1; |
else case <OC:Thermococcus>
Catalytic activity |
Reaction=ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.; EC=6.5.1.6; |
|
Reaction=NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.; EC=6.5.1.6; |
else case <OC:Bacteria>
Catalytic activity |
Reaction=ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.; EC=6.5.1.1; |
end case
Cofactor |
Mg(2+) |
Similarity |
Belongs to the ATP-dependent DNA ligase family. |
case <OC:Thermococcus>
end case
From: DNLI_PYRFU (P56709) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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ACT_SITE
|
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249
|
|
249
|
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N6-AMP-lysine intermediate
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|
|
|
K
|
|
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BINDING
|
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247
|
|
247
|
|
ATP
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|
|
[DE]
|
|
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BINDING
|
|
254
|
|
254
|
|
ATP
|
|
|
|
R
|
|
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BINDING
|
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269
|
|
269
|
|
ATP
|
|
|
|
R
|
|
|
BINDING
|
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299
|
|
299
|
|
ATP
|
|
|
|
E
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BINDING
|
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339
|
|
339
|
|
ATP
|
|
|
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F
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BINDING
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414
|
|
414
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|
ATP
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R
|
|
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BINDING
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420
|
|
420
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ATP
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K
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Additional information
[?]
Size range |
503-618 amino acids |
Related rules |
None |
Fusion |
Nter: <Unknown>; Cter: None |
Comments |
Shorter N-terminus in the first copy of lig in METBF. Some archaea use additional nucleotide cofactors (such as NAD(+), ADP or GTP) next to ATP. |