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Annotation rule MF_00418
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General rule information [?]

Accession MF_00418
Dates 19-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 41)
Name DapA
Scope
Bacteria
Archaea
Templates P0A6L2 (DAPA_ECOLI); P9WP25 (DAPA_MYCTU); Q9X1K9 (DAPA_THEMA); Q57695 (DAPA_METJA); Q6GH13 (DAPA_STAAR); Q5HG25 (DAPA_STAAC); Q9JZR4 (DAPA_NEIMB); Q9I4W3 (DAPA_PSEAE); Q07607 (DAPA_RHIML); Q8UGL3 (DAPA_AGRFC): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
DAPA
Protein name
RecName: Full=4-hydroxy-tetrahydrodipicolinate synthase;
Short=HTPA synthase;
EC 4.3.3.7;
Gene name
dapA

Comments [?]

Function Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).
Catalytic activity RHEA:34171: L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O
EC 4.3.3.7
Pathway Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.
case (<OC:Bacteria> or <OC:Archaea>) and not (<OC:Staphylococcus> or <OC:Pseudomonas>)
Subunit Homotetramer; dimer of dimers.
else
Subunit Homodimer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the DapA family.
Caution Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Keywords [?]


Gene Ontology [?]

GO:0008840; Molecular function: 4-hydroxy-tetrahydrodipicolinate synthase activity.
GO:0019877; Biological process: diaminopimelate biosynthetic process.
GO:0009085; Biological process: lysine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00701; DHDPS; 1;
PRINTS PR00146; DHPICSNTHASE; 1;
TIGRFAMs TIGR00674; DapA; 1;
PROSITE PS00665; DHDPS_1; 1;
PS00666; DHDPS_2; 1;

Features [?]

From: DAPA_ECOLI (P0A6L2)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     133     133       Proton donor/acceptor     Y  
ACT_SITE     161     161       Schiff-base intermediate with substrate     K  
BINDING     45     45       Pyruvate     [TS]  
BINDING     203     203       Pyruvate; via carbonyl oxygen     [IVAT]  
SITE     44     44       Part of a proton relay during catalysis     [TS]  
SITE     107     107       Part of a proton relay during catalysis     Y  

Additional information [?]

Size range 283-311 amino acids
Related rules MF_01237 (NANA supersedes the current rule); MF_00694 (KDGD)
Fusion None
Comments None.