HAMAP rule MF_00418
General rule information
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| Accession | MF_00418 |
| Dates | 19-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 46) |
| Name | DapA |
| Scope | Bacteria
Archaea |
| Templates | P0A6L2 (DAPA_ECOLI); P9WP25 (DAPA_MYCTU); Q9X1K9 (DAPA_THEMA); Q57695 (DAPA_METJA); Q6GH13 (DAPA_STAAR); Q5HG25 (DAPA_STAAC); Q9JZR4 (DAPA_NEIMB); Q9I4W3 (DAPA_PSEAE); Q07607 (DAPA_RHIML); Q8UGL3 (DAPA_AGRFC): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA). |
| Catalytic activity | RHEA:34171: L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O
EC 4.3.3.7 |
| Pathway | Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. |
case (<OC:Bacteria> or <OC:Archaea>) and not (<OC:Staphylococcus> or <OC:Pseudomonas>)
| Subunit | Homotetramer; dimer of dimers. |
else
| Subunit | Homodimer. |
end case
| Subcellular location | Cytoplasm. |
| Similarity | Belongs to the DapA family. |
| Caution | Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB. |
Keywords
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Cytoplasm
Amino-acid biosynthesis
Diaminopimelate biosynthesis
Lyase
Lysine biosynthesis
Schiff base
Amino-acid biosynthesis
Diaminopimelate biosynthesis
Lyase
Lysine biosynthesis
Schiff base
Gene Ontology
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GO:0008840; Molecular function: 4-hydroxy-tetrahydrodipicolinate synthase activity.
GO:0019877; Biological process: diaminopimelate biosynthetic process.
GO:0009085; Biological process: lysine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
GO:0019877; Biological process: diaminopimelate biosynthetic process.
GO:0009085; Biological process: lysine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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| Pfam | PF00701; DHDPS; 1; |
| PRINTS | PR00146; DHPICSNTHASE; 1; |
| NCBIfam | TIGR00674; DapA; 1; |
| PROSITE | PS00665; DHDPS_1; 1; |
| PS00666; DHDPS_2; 1; |
Features
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| From: DAPA_ECOLI (P0A6L2) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 133 | 133 | Proton donor/acceptor | Y | ||||||||
| ACT_SITE | 161 | 161 | Schiff-base intermediate with substrate | K | ||||||||
| BINDING | 45 | 45 | /ligand="pyruvate" /ligand_id="ChEBI:CHEBI:15361 | [TS] | ||||||||
| BINDING | 203 | 203 | /ligand="pyruvate" /ligand_id="ChEBI:CHEBI:15361 | [IVAT] | ||||||||
| SITE | 44 | 44 | Part of a proton relay during catalysis | [TS] | ||||||||
| SITE | 107 | 107 | Part of a proton relay during catalysis | Y | ||||||||
Additional information
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| Size range | 283-311 amino acids |
| Related rules | MF_01237 (NANA supersedes the current rule); MF_00694 (KDGD) |
| Fusion | None |
| Comments | None. |