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HAMAP rule MF_00419

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General rule information [?]

Accession MF_00419
Dates 25-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 47)
Name PurL_1
Scope(s) Bacteria
Template(s) P15254 (PUR4_ECOLI); P74881 (PUR4_SALTY); [ Recover all ]
Triggered by HAMAP; MF_00419 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PUR4
Protein name RecName: Full=Phosphoribosylformylglycinamidine synthase;
                 Short=FGAM synthase;
                 Short=FGAMS;
                 EC=6.3.5.3;
AltName: Full=Formylglycinamide ribonucleotide amidotransferase;
                 Short=FGAR amidotransferase;
                 Short=FGAR-AT;
Gene name Name=purL;

Comments [?]

FUNCTIONPhosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
CATALYTIC ACTIVITY Reaction=ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D- ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D- ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:17129, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:147286, ChEBI:CHEBI:147287, ChEBI:CHEBI:456216; EC=6.3.5.3;
PATHWAYPurine metabolism; IMP biosynthesis via de novo pathway; 5- amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5- phospho-D-ribosyl)glycinamide: step 1/2.
SUBUNITMonomer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYIn the N-terminal section; belongs to the FGAMS family.

Keywords [?]

ATP-binding
Cytoplasm
Glutamine amidotransferase
Ligase
Metal-binding
Magnesium
Nucleotide-binding
Purine biosynthesis

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004642; Molecular function:phosphoribosylformylglycinamidine synthase activity
GO:0006189; Biological process:'de novo' IMP biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00586; AIRS; 1;
PROSITE PS51273; GATASE_TYPE_1; 1;
NCBIfam TIGR01735; FGAM_synt; 1;

Features [?]

From: PUR4_SALTY (P74881)
Key From To Description Tag Condition FTGroup
BINDING 307 318 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 G-A-[SA]-T-G-[SAVI]-G-G-E-I-R-D
BINDING 386 388 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 T-G-Y
ACT_SITE 1135 1135 /note="Nucleophile" C
ACT_SITE 1260 1260 H
ACT_SITE 1262 1262 E
BINDING 679 679 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D
BINDING 718 718 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 E
BINDING 722 722 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 N
BINDING 884 884 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D
BINDING 678 678 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 A
BINDING 886 886 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 S

Additional information [?]

Size range 1293-1369 amino acids
Related rules MF_00420
MF_00421
Fusion Nter: None Cter: None
Comments FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926).



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