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Annotation rule MF_00419
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General rule information [?]

Accession MF_00419
Dates 25-JUN-2001 (Created)
23-MAR-2020 (Last updated, Version 44)
Name PurL_1
Templates P15254 (PUR4_ECOLI); P74881 (PUR4_SALTY): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Phosphoribosylformylglycinamidine synthase;
Short=FGAM synthase;
AltName: Full=Formylglycinamide ribonucleotide amidotransferase;
Short=FGAR amidotransferase;
Gene name

Comments [?]

Function Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Catalytic activity RHEA:17129: ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate
Pathway Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity In the N-terminal section; belongs to the FGAMS family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004642; Molecular function: phosphoribosylformylglycinamidine synthase activity.
GO:0006189; Biological process: 'de novo' IMP biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00586; AIRS; 1;
PROSITE PS51273; GATASE_TYPE_1; 1; trigger=PRU00605;
TIGRFAMs TIGR01735; FGAM_synt; 1;

Features [?]

From: PUR4_SALTY (P74881)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     307     318       ATP     G-A-[SA]-T-G-[SAVI]-G-G-E-I-R-D  
NP_BIND (Optional)     386     388       ATP     T-G-Y  
ACT_SITE     1135     1135       Nucleophile     C  
ACT_SITE     1260     1260             H  
ACT_SITE     1262     1262             E  
METAL     679     679       Magnesium     D  
METAL     718     718       Magnesium     E  
METAL     722     722       Magnesium     N  
METAL     884     884       Magnesium     D  
BINDING     678     678       ATP; via carbonyl oxygen     A  
BINDING     886     886       ATP     S  

Additional information [?]

Size range 1293-1369 amino acids
Related rules MF_00420 (PURL); MF_00421 (PURQ)
Fusion None
Comments FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926).