HAMAP rule MF_00452

General rule information [?]

Accession	MF_00452
Dates	23-SEP-2001 (Created) 19-NOV-2022 (Last updated, Version 38)

Name

PEPCK_GTP

Scope

Bacteria

Archaea

Templates

Q9AEM1 (PCKG_CORGL); Q9AGJ6 (PCKG_MYCSM); Q6F494 (PCKG_THEKO); A5TYT6 (PCKG_MYCTA): [Recover all]

Triggered by

HAMAP; MF_00452 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PCKG

Protein name

RecName:		Full=Phosphoenolpyruvate carboxykinase [GTP];
		Short=PEP carboxykinase;
		Short=PEPCK;
		EC 4.1.1.32;
AltName:		Full=GTP-dependent phosphoenolpyruvate carboxykinase;
		Short=GTP-PEPCK;

Gene name

pckG

Comments [?]

case <OS:Treponema pallidum>

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP).

else

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

end case

Catalytic activity

RHEA:10388: GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate
EC 4.1.1.32

case <FTGroup:1>

Cofactor

Mn(2+)
Note: Binds 1 Mn(2+) ion per subunit.

end case

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

case <OC:Bacteria>

Subunit

Monomer.

end case

Subcellular location	Cytoplasm.
Similarity	Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Keywords [?]

Cytoplasm
Decarboxylase
Gluconeogenesis
GTP-binding
Lyase

case <FTGroup:1>

Manganese
Metal-binding

end case

Nucleotide-binding

Gene Ontology [?]

GO:0005525; Molecular function: GTP binding.
GO:0004613; Molecular function: phosphoenolpyruvate carboxykinase (GTP) activity.

case <FTGroup:1>

GO:0030145; Molecular function: manganese ion binding.

end case

GO:0006094; Biological process: gluconeogenesis.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF00821; PEPCK; 1;
PROSITE	PS00505; PEPCK_GTP; 1;

Features [?]

From: PCKG_MYCTA (A5TYT6)

Key From To Description Tag Condition FTGroup

BINDING (Optional) 272 277 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 [AGSQM]-[CS]-G-K-T-[NS]

BINDING (Optional) 515 518 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 [FY]-x-x-N

BINDING 220 222 /ligand="substrate Y-x-G

BINDING 387 389 /ligand="substrate N-x-R

ACT_SITE 273 273 [CS]

BINDING 229 229 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035 K 1

BINDING 249 249 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035 H 1

BINDING 296 296 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035 D 1

BINDING 81 81 /ligand="substrate R

BINDING 271 271 /ligand="substrate S

BINDING 389 389 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 R

BINDING 420 420 /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 R

Additional information [?]

Size range	588-624 amino acids
Related rules	None
Fusion	None
Comments	The enzyme from THEKO is shown to be a homotetramer.

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