HAMAP rule MF_00452
General rule information
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| Accession | MF_00452 |
| Dates | 23-SEP-2001 (Created)
19-NOV-2022 (Last updated, Version 38) |
| Name | PEPCK_GTP |
| Scope(s) |
Bacteria Archaea |
| Template(s) | Q9AEM1 (PCKG_CORGL); Q9AGJ6 (PCKG_MYCSM); Q6F494 (PCKG_THEKO); A5TYT6 (PCKG_MYCTA); [ Recover all ] |
| Triggered by |
HAMAP; MF_00452 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PCKG |
| Protein name | RecName: Full=Phosphoenolpyruvate carboxykinase [GTP]; Short=PEP carboxykinase; Short=PEPCK; EC=4.1.1.32; AltName: Full=GTP-dependent phosphoenolpyruvate carboxykinase; Short=GTP-PEPCK; |
| Gene name | Name=pckG; |
Comments
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| case <OS:Treponema pallidum> | |
| FUNCTION | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP). |
| else | |
| FUNCTION | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate; Xref=Rhea:RHEA:10388, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526, ChEBI:CHEBI:37565, ChEBI:CHEBI:58189, ChEBI:CHEBI:58702; EC=4.1.1.32; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 1 Mn(2+) ion per subunit.; |
| end case | |
| PATHWAY | Carbohydrate biosynthesis; gluconeogenesis. |
| case <OC:Bacteria> | |
| SUBUNIT | Monomer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. |
Keywords
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| Cytoplasm | |
| Decarboxylase | |
| Gluconeogenesis | |
| GTP-binding | |
| Lyase | |
| case <FTGroup:1> | |
| Manganese | |
| Metal-binding | |
| end case | |
| Nucleotide-binding | |
Gene Ontology
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| GO:0005525; Molecular function:GTP binding | |
| GO:0004613; Molecular function:phosphoenolpyruvate carboxykinase (GTP) activity | |
| case <FTGroup:1> | |
| GO:0030145; Molecular function:manganese ion binding | |
| end case | |
| GO:0006094; Biological process:gluconeogenesis | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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Features
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| From: PCKG_MYCTA (A5TYT6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 272 | 277 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[AGSQM]-[CS]-G-K-T-[NS] | ||||||||
| BINDING | 515 | 518 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[FY]-x-x-N | ||||||||
| BINDING | 220 | 222 | /ligand="substrate" | Y-x-G | ||||||||
| BINDING | 387 | 389 | /ligand="substrate" | N-x-R | ||||||||
| ACT_SITE | 273 | 273 | [CS] | |||||||||
| BINDING | 229 | 229 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
K | 1 | |||||||
| BINDING | 249 | 249 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
H | 1 | |||||||
| BINDING | 296 | 296 | /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035" |
D | 1 | |||||||
| BINDING | 81 | 81 | /ligand="substrate" | R | ||||||||
| BINDING | 271 | 271 | /ligand="substrate" | S | ||||||||
| BINDING | 389 | 389 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
R | ||||||||
| BINDING | 420 | 420 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
R | ||||||||
Additional information
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| Size range | 588-624 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | The enzyme from THEKO is shown to be a homotetramer. |