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Annotation rule MF_00453
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General rule information [?]

Accession MF_00453
Dates 25-SEP-2001 (Created)
18-NOV-2019 (Last updated, Version 30)
Name PEPCK_ATP
Scope
Bacteria
Archaea
Templates P22259 (PCKA_ECOLI); A6VKV4 (PCKA_ACTSZ); O09460 (PCKA_ANASU); Q5SLL5 (PCKA_THET8): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PCKA
Protein name
RecName: Full=Phosphoenolpyruvate carboxykinase (ATP);
Short=PEP carboxykinase;
Short=PEPCK;
EC 4.1.1.49;
Short=PCK;
Gene name
pckA

Comments [?]

Function Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.
Catalytic activity RHEA:18617: ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate
EC 4.1.1.49
Cofactor Mn(2+)
Note: Binds 1 Mn(2+) ion per subunit.
Pathway Carbohydrate biosynthesis; gluconeogenesis.
case <OC:Gammaproteobacteria>
Subunit Monomer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.

Keywords [?]

case <FT:15> or <FT:16>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004612; Molecular function: phosphoenolpyruvate carboxykinase (ATP) activity.
GO:0006094; Biological process: gluconeogenesis.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01293; PEPCK_ATP; 1;
TIGRFAMs TIGR00224; PckA; 1;
PROSITE PS00532; PEPCK_ATP; 1;

Features [?]

From: PCKA_ECOLI (P22259)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     248     256       ATP     G-L-S-G-T-G-K-T-T  
NP_BIND     449     450       ATP     [RK]-[IV]  
METAL     213     213       Manganese     K  
METAL     232     232       Manganese; via tele nitrogen     H  
METAL     269     269       Manganese     D  
BINDING     65     65       Substrate     R  
BINDING     207     207       Substrate     [YF]  
BINDING     213     213       ATP     K  
BINDING     213     213       Substrate     K  
BINDING     232     232       ATP     H  
BINDING     297     297       ATP     [ED]  
BINDING     333     333       ATP     R  
BINDING     333     333       Substrate     R  
BINDING     455     455       ATP     [TS]  
case <OC:Escherichia> or <OC:Shigella>
MOD_RES     87     87       N6-acetyllysine     K  
MOD_RES     523     523       N6-acetyllysine     K  
end case

Additional information [?]

Size range 493-542 amino acids
Related rules None
Fusion None
Comments Probable C-terminally frameshifted DEIRA not taken into alignment and size range. Allosteric regulation by calcium ions is showed only in E.coli, S. typhimurium and T.thermophilus.