HAMAP rule MF_00463

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00463
Accession	MF_00463
Dates	28-FEB-2005 (Created) 01-JUN-2023 (Last updated, Version 42)
Name	RsxB_RnfB
Scope(s)	Archaea Bacteria
Template(s)	P77223; P0CZ14; D5ARZ0; A5F2R3; H6LC27; D8GR71; Q8TSX9; [ Recover all ]
Triggered by	HAMAP; MF_00463 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Identifier	RSXB
Protein name	RecName: Full=Ion-translocating oxidoreductase complex subunit B; EC=7.-.-.-; AltName: Full=Rsx electron transport complex subunit B;
Gene name	Name=rsxB;
else
Identifier	RNFB
Protein name	RecName: Full=Ion-translocating oxidoreductase complex subunit B; EC=7.-.-.-; AltName: Full=Rnf electron transport complex subunit B;
Gene name	Name=rnfB;
end case

Comments [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
FUNCTION	Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane. Required to maintain the reduced state of SoxR.
else
FUNCTION	Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.
end case
case <FTGroup:1> and (<FTGroup:2> or <FTGroup:4>) and <FTGroup:3> and not <FTGroup:5>
COFACTOR	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 3 [4Fe-4S] clusters
else case <FTGroup:1> and (<FTGroup:2> or <FTGroup:4>) and <FTGroup:3> and <FTGroup:5>
COFACTOR	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 4 [4Fe-4S] clusters
else
COFACTOR	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
end case
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
SUBUNIT	The complex is composed of six subunits: RsxA, RsxB, RsxC, RsxD, RsxE and RsxG.
else case <OC:Bacteria>
SUBUNIT	The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.
else case <OC:Archaea>
SUBUNIT	The Rnf complex is probably composed of eight subunits, including RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.
end case
case (<OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)) and not <OC:Paracoccaceae>
SUBCELLULAR LOCATION	Cell inner membrane.
else case <OC:Paracoccaceae>
SUBCELLULAR LOCATION	Cellular chromatophore membrane; Peripheral membrane protein; Cytoplasmic side.
else
SUBCELLULAR LOCATION	Cell membrane.
end case
SIMILARITY	Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfB subfamily.

Keywords [?]

4Fe-4S
case (<OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)) and not <OC:Paracoccaceae>
Cell inner membrane
end case
case not <OC:Paracoccaceae>
Cell membrane
end case
Electron transport
Iron
Iron-sulfur
Membrane
Metal-binding
case <OC:Paracoccaceae>
Nitrogen fixation
end case
Repeat
Translocase
Transport

Gene Ontology [?]

GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:0022900; Biological process:electron transport chain
case <OCellular component:Paracoccaceae>
GO:0009399; Biological process:nitrogen fixation
end case
case not <OCellular component:Paracoccaceae>
GO:0005886; Cellular component:plasma membrane
end case

Cross-references [?]

Pfam	PF00037; Fer4; 2;
Pfam	PF04060; FeS; 1;
PRINTS	PR00353; 4FE4SFRDOXIN; 1;
NCBIfam	TIGR01944; rnfB; 1;
PROSITE	PS51656; 4FE4S; 1;
PROSITE	PS00198; 4FE4S_FER_1; 2;
PROSITE	PS51379; 4FE4S_FER_2; 2;

Features [?]

From: RNFB_RHOCA (P0CZ14)
Key	From	To	Description	Tag	Condition	FTGroup
REGION	Nter	23	/note="Hydrophobic"
BINDING	46	46	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1"		C	1
BINDING	49	49	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1"		C	1
BINDING	54	54	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1"		C	1
BINDING	71	71	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1"		C	1
BINDING	112	112	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	2
BINDING	115	115	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	2
BINDING	118	118	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	2
BINDING	122	122	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="3"		C	3
BINDING	142	142	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="3"		C	3
BINDING	145	145	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="3"		C	3
BINDING	148	148	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="3"		C	3
BINDING	152	152	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	2
From: RNFB_ACEWD (H6LC27)
Key	From	To	Description	Tag	Condition	FTGroup
BINDING	138	138	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	4
BINDING	142	142	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	4
BINDING	148	148	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	4
BINDING	182	182	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2"		C	4
BINDING	217	217	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="4"		C	5
BINDING	220	220	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="4"		C	5
BINDING	223	223	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="4"		C	5
BINDING	227	227	/ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="4"		C	5

Additional information [?]

Size range	160-400 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	According to PubMed:9154934, this protein is a peripheral membrane protein, and the N-terminal hydrophobic stretch is not a membrane anchor. According to PubMed:25831459, this protein is a transmembrane protein.

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