HAMAP rule MF_00463

General rule information [?]

Accession	MF_00463
Dates	15-OCT-2001 (Created) 26-JAN-2023 (Last updated, Version 51)

Name

RsxB_RnfB

Scope

Archaea

Bacteria

Templates

P77223 (RSXB_ECOLI); P0CZ14 (RNFB_RHOCA); D5ARZ0 (RNFB_RHOCB); A5F2R3 (RNFB_VIBC3); H6LC27 (RNFB_ACEWD); D8GR71 (RNFB_CLOLD); Q8TSX9 (RNFB_METAC): [Recover all]

Triggered by

HAMAP; MF_00463 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>

Identifier

RSXB

Protein name

RecName:		Full=Ion-translocating oxidoreductase complex subunit B;
		EC 7.-.-.-;
AltName:		Full=Rsx electron transport complex subunit B;

Gene name

rsxB

else

Identifier

RNFB

Protein name

RecName:		Full=Ion-translocating oxidoreductase complex subunit B;
		EC 7.-.-.-;
AltName:		Full=Rnf electron transport complex subunit B;

Gene name

rnfB

end case

Comments [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>

Function

Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane. Required to maintain the reduced state of SoxR.

else

Function

Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.

end case

case <FTGroup:1> and (<FTGroup:2> or <FTGroup:4>) and <FTGroup:3> and not <FTGroup:5>

Cofactor

[4Fe-4S] cluster
Note: Binds 3 [4Fe-4S] clusters

else case <FTGroup:1> and (<FTGroup:2> or <FTGroup:4>) and <FTGroup:3> and <FTGroup:5>

Cofactor

[4Fe-4S] cluster
Note: Binds 4 [4Fe-4S] clusters

else

Cofactor

[4Fe-4S] cluster

end case

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>

Subunit

The complex is composed of six subunits: RsxA, RsxB, RsxC, RsxD, RsxE and RsxG.

else case <OC:Bacteria>

Subunit

The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.

else case <OC:Archaea>

Subunit

The Rnf complex is probably composed of eight subunits, including RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.

end case

case (<OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)) and not <OC:Paracoccaceae>

Subcellular location

Cell inner membrane.

else case <OC:Paracoccaceae>

Subcellular location

Cellular chromatophore membrane; Peripheral membrane protein; Cytoplasmic side.

else

Subcellular location

Cell membrane.

end case

Similarity

Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfB subfamily.

Keywords [?]

4Fe-4S

case (<OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)) and not <OC:Paracoccaceae>

Cell inner membrane

end case

case not <OC:Paracoccaceae>

Cell membrane

end case

Electron transport
Iron
Iron-sulfur
Membrane
Metal-binding

case <OC:Paracoccaceae>

Nitrogen fixation

end case

Repeat
Translocase
Transport

Gene Ontology [?]

GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0022900; Biological process: electron transport chain.

case <OC:Paracoccaceae>

GO:0009399; Biological process: nitrogen fixation.

end case

case not <OC:Paracoccaceae>

GO:0005886; Cellular component: plasma membrane.

end case

Cross-references [?]

Pfam	PF00037; Fer4; 2;
	PF04060; FeS; 1;
PRINTS	PR00353; 4FE4SFRDOXIN; 1;
TIGRFAMs	TIGR01944; rnfB; 1;
PROSITE	PS51656; 4FE4S; 1; trigger=PRU00989;
	PS00198; 4FE4S_FER_1; 2;
	PS51379; 4FE4S_FER_2; 2; trigger=PRU00711;

Features [?]

From: RNFB_RHOCA (P0CZ14)

Key From To Description Tag Condition FTGroup

REGION Nter 23 Hydrophobic

BINDING 46 46 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 49 49 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 54 54 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 71 71 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING (Optional) 112 112 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 2

BINDING (Optional) 115 115 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 2

BINDING (Optional) 118 118 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 2

BINDING 122 122 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="3 C 3

BINDING 142 142 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="3 C 3

BINDING 145 145 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="3 C 3

BINDING 148 148 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="3 C 3

BINDING (Optional) 152 152 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 2

From: RNFB_ACEWD (H6LC27)

BINDING (Optional) 138 138 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 4

BINDING (Optional) 142 142 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 4

BINDING (Optional) 148 148 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 4

BINDING (Optional) 182 182 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2 C 4

BINDING (Optional) 217 217 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="4 C 5

BINDING (Optional) 220 220 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="4 C 5

BINDING (Optional) 223 223 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="4 C 5

BINDING (Optional) 227 227 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="4 C 5

Additional information [?]

Size range	160-400 amino acids
Related rules	None
Fusion	None
Comments	According to PubMed:9154934, this protein is a peripheral membrane protein, and the N-terminal hydrophobic stretch is not a membrane anchor. According to PubMed:25831459, this protein is a transmembrane protein.

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