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HAMAP rule MF_00488

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General rule information [?]

Accession MF_00488
Dates 23-NOV-2001 (Created)
19-NOV-2022 (Last updated, Version 48)
Name Lactate_dehydrog
Scope
Bacteria
Templates P0CW93 (LDH2_BIFLO); P00344 (LDH_GEOSE); P00343 (LDH_LACCA); O32765 (LDH_LACHE); P16115 (LDH_THEMA): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
LDH
Protein name
RecName: Full=L-lactate dehydrogenase;
Short=L-LDH;
EC 1.1.1.27;
Gene name
ldh

Comments [?]

Function Catalyzes the conversion of lactate to pyruvate.
Catalytic activity RHEA:23444: (S)-lactate + NAD(+) = H(+) + NADH + pyruvate
EC 1.1.1.27
case <FTGroup:1>
Activity regulation Allosterically activated by fructose 1,6-bisphosphate (FBP).
end case
Pathway Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit Homotetramer.
Subcellular location Cytoplasm.
Similarity Belongs to the LDH/MDH superfamily. LDH family.

Keywords [?]

case <FTGroup:1>
Allosteric enzyme
end case
Cytoplasm
Oxidoreductase
NAD
case <FTTag:Phosphotyrosine>
Phosphoprotein
end case
case <OS:Staphylococcus aureus>
Stress response
end case

Gene Ontology [?]

GO:0004459; Molecular function: L-lactate dehydrogenase activity.
GO:0006096; Biological process: glycolytic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF02866; Ldh_1_C; 1;
PF00056; Ldh_1_N; 1;
PRINTS PR00086; LLDHDRGNASE; 1;
TIGRFAMs TIGR01771; L-LDH-NAD; 1;
TIGR01763; MalateDH_bact; 1;
PROSITE PS00064; L_LDH; 1;

Features [?]

From: LDH_GEOSE (P00344)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     83     84       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     G-[AVL]  
BINDING     122     124       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     [AIV]-x-N  
BINDING     124     127       /ligand="substrate     N-x-x-D  
BINDING     152     155       /ligand="substrate     D-x-x-R  
ACT_SITE     179     179       Proton acceptor     H  
BINDING     17     17       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     V  
BINDING     38     38       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     [DNE]  
BINDING     43     43       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     [RK]  
BINDING     69     69       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     Y  
BINDING     86     86       /ligand="substrate     Q  
BINDING     92     92       /ligand="substrate     R  
BINDING     105     105       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     [ST]  
BINDING     147     147       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     [ST]  
BINDING (Optional)     157     157       /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="allosteric activator     [RK]   1
BINDING (Optional)     172     172       /ligand="beta-D-fructose 1,6-bisphosphate" /ligand_id="ChEBI:CHEBI:32966" /ligand_note="allosteric activator     H   1
BINDING     233     233       /ligand="substrate     T  
From: LDH_BACSU (P13714)
MOD_RES (Optional)     223     223       Phosphotyrosine   Phosphotyrosine   Y  

Additional information [?]

Size range 304-330 amino acids
Related rules MF_00487 (MDH); MF_01516 (MDH); MF_01517 (MDH)
Fusion None


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