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HAMAP rule MF_00488

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General rule information [?]

Accession MF_00488
Dates 23-NOV-2001 (Created)
1-JUN-2023 (Last updated, Version 49)
Name Lactate_dehydrog
Scope(s) Bacteria
Template(s) P0CW93 (LDH2_BIFLO); P00344 (LDH_GEOSE); P00343 (LDH_LACCA); O32765 (LDH_LACHE); P16115 (LDH_THEMA); [ Recover all ]
Triggered by HAMAP; MF_00488 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier LDH
Protein name RecName: Full=L-lactate dehydrogenase;
                 Short=L-LDH;
                 EC=1.1.1.27;
Gene name Name=ldh;

Comments [?]

FUNCTIONCatalyzes the conversion of lactate to pyruvate.
CATALYTIC ACTIVITY Reaction=(S)-lactate + NAD(+) = H(+) + NADH + pyruvate; Xref=Rhea:RHEA:23444, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:16651, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.27;
case <FTGroup:1>
ACTIVITY REGULATIONAllosterically activated by fructose 1,6- bisphosphate (FBP).
end case
PATHWAYFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
SUBUNITHomotetramer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the LDH/MDH superfamily. LDH family.

Keywords [?]

case <FTGroup:1>
Allosteric enzyme
end case
Cytoplasm
Oxidoreductase
NAD
case <FTTag:Phosphotyrosine>
Phosphoprotein
end case
case <OS:Staphylococcus aureus>
Stress response
end case

Gene Ontology [?]

GO:0004459; Molecular function:L-lactate dehydrogenase activity
GO:0006096; Biological process:glycolytic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF02866; Ldh_1_C; 1;
Pfam PF00056; Ldh_1_N; 1;
PRINTS PR00086; LLDHDRGNASE; 1;
NCBIfam TIGR01771; L-LDH-NAD; 1;
NCBIfam TIGR01763; MalateDH_bact; 1;
PROSITE PS00064; L_LDH; 1;

Features [?]

From: LDH_GEOSE (P00344)
Key From To Description Tag Condition FTGroup
BINDING 83 84 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
G-[AVL]
BINDING 122 124 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
[AIV]-x-N
BINDING 124 127 /ligand="substrate" N-x-x-D
BINDING 152 155 /ligand="substrate" D-x-x-R
ACT_SITE 179 179 /note="Proton acceptor" H
BINDING 17 17 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
V
BINDING 38 38 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
[DNE]
BINDING 43 43 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
[RK]
BINDING 69 69 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
Y
BINDING 86 86 /ligand="substrate" Q
BINDING 92 92 /ligand="substrate" R
BINDING 105 105 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
[ST]
BINDING 147 147 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"
[ST]
BINDING 157 157 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="allosteric activator"
[RK] 1
BINDING 172 172 /ligand="beta-D-fructose 1,6-bisphosphate"
/ligand_id="ChEBI:CHEBI:32966"
/ligand_note="allosteric activator"
H 1
BINDING 233 233 /ligand="substrate" T
From: LDH_BACSU (P13714)
Key From To Description Tag Condition FTGroup
MOD_RES 223 223 /note="Phosphotyrosine" Phosphotyrosine Y

Additional information [?]

Size range 304-330 amino acids
Related rules MF_00487
MF_01516
MF_01517
Fusion Nter: None Cter: None



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