HAMAP rule MF_00495
General rule information
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| Accession | MF_00495 |
| Dates | 29-NOV-2001 (Created)
14-MAY-2024 (Last updated, Version 40) |
| Name | GPH_hydrolase_bact |
| Scope(s) |
Bacteria |
| Template(s) | P32662 (GPH_ECOLI); P40852 (GPHC_CUPNH); P40853 (GPHP_CUPNH); [ Recover all ] |
| Triggered by |
HAMAP; MF_00495 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GPH |
| Protein name | RecName: Full=Phosphoglycolate phosphatase; Short=PGP; Short=PGPase; EC=3.1.3.18; |
| case not <Property:PHOTOSYN> | |
| Gene name | Name=gph; |
| end case | |
| case <Property:PHOTOSYN> | |
| Gene name | Name=cbbZ; |
| end case | |
Comments
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| case <OC:Pseudomonadota> | |
| FUNCTION | Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress. |
| end case | |
| case not <OC:Pseudomonadota> | |
| FUNCTION | Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=2-phosphoglycolate + H2O = glycolate + phosphate; Xref=Rhea:RHEA:14369, ChEBI:CHEBI:15377, ChEBI:CHEBI:29805, ChEBI:CHEBI:43474, ChEBI:CHEBI:58033; EC=3.1.3.18; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| case <OC:Enterobacterales> | |
| COFACTOR | Name=chloride; Xref=ChEBI:CHEBI:17996; |
| SUBUNIT | Monomer. |
| end case | |
| PATHWAY | Organic acid metabolism; glycolate biosynthesis; glycolate from 2-phosphoglycolate: step 1/1. |
| SIMILARITY | Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family. |
Keywords
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| Carbohydrate metabolism | |
| case <Property:PHOTOSYN> | |
| Calvin cycle | |
| Photosynthesis | |
| end case | |
| Hydrolase | |
| Magnesium | |
| Metal-binding | |
| case <OC:Enterobacterales> | |
| Chloride | |
| end case | |
Gene Ontology
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| GO:0008967; Molecular function:phosphoglycolate phosphatase activity | |
| GO:0046295; Biological process:glycolate biosynthetic process | |
| case <Property:PHOTOSYN> | |
| GO:0019253; Biological process:reductive pentose-phosphate cycle | |
| end case | |
Cross-references
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Features
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| From: GPH_ECOLI (P32662) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 13 | 13 | /note="Nucleophile" | D | ||||||||
| BINDING | 13 | 13 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 15 | 15 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 192 | 192 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
Additional information
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| Size range | 212-272 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Possible overlap with other paralogous genes in many genomes. Divergent gph in SYNE7 not shown in alignment as it could well be a paralog. |