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Annotation rule MF_00518
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General rule information [?]

Accession MF_00518
Dates 17-DEC-2001 (Created)
18-NOV-2019 (Last updated, Version 29)
Name Deacylase_Dtd
Templates P0A6M4 (DTD_ECOLI); Q8IIS0 (DTD_PLAF7): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=D-aminoacyl-tRNA deacylase;
AltName: Full=Gly-tRNA(Ala) deacylase;
EC 3.1.1.-;
Gene name

Comments [?]

Function An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.
Catalytic activity RHEA:13953: a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + H(+) + tRNA
RHEA:53744: glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)
Subunit Homodimer.
Subcellular location Cytoplasm.
case <FTTag:motif>
Domain A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.
end case
Similarity Belongs to the DTD family.

Keywords [?]

Gene Ontology [?]

GO:0016788; Molecular function: hydrolase activity, acting on ester bonds.
GO:0019478; Biological process: D-amino acid catabolic process.
GO:0051499; Molecular function: D-aminoacyl-tRNA deacylase activity.
GO:0005737; Cellular component: cytoplasm.
GO:0000049; Molecular function: tRNA binding.
GO:0043908; Molecular function: Ser(Gly)-tRNA(Ala) hydrolase activity.
GO:0106026; Molecular function: Gly-tRNA(Ala) hydrolase activity.

Cross-references [?]

Pfam PF02580; Tyr_Deacylase; 1;
TIGRFAMs TIGR00256; TIGR00256; 1;

Features [?]

From: DTD_ECOLI (P0A6M4)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     137     138       Gly-cisPro motif, important for rejection of L-amino acids   motif   G-P  

Additional information [?]

Size range 127-200 amino acids
Related rules None
Fusion None
Comments BACSU (O32042) is inactive due to missing start codon, SHIF8 (Q0SZ73) may have a sequence error