HAMAP rule MF_00518
General rule information
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| Accession | MF_00518 |
| Dates | 17-DEC-2001 (Created)
3-SEP-2024 (Last updated, Version 32) |
| Name | Deacylase_Dtd |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A6M4 (DTD_ECOLI); Q8IIS0 (DTD_PLAF7); [ Recover all ] |
| Triggered by |
HAMAP; MF_00518 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DTD |
| Protein name | RecName: Full=D-aminoacyl-tRNA deacylase; Short=DTD; EC=3.1.1.96; AltName: Full=Gly-tRNA(Ala) deacylase; EC=3.1.1.-; |
| Gene name | Name=dtd; |
Comments
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| FUNCTION | An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality. |
| CATALYTIC ACTIVITY | Reaction=a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+); Xref=Rhea:RHEA:13953, Rhea:RHEA-COMP:10123, Rhea:RHEA- COMP:10124, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:59871, ChEBI:CHEBI:78442, ChEBI:CHEBI:79333; EC=3.1.1.96; |
| CATALYTIC ACTIVITY | Reaction=glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+); Xref=Rhea:RHEA:53744, Rhea:RHEA-COMP:9657, Rhea:RHEA-COMP:13640, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57305, ChEBI:CHEBI:78442, ChEBI:CHEBI:78522; |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| case <FTTag:motif> | |
| DOMAIN | A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids. |
| end case | |
| SIMILARITY | Belongs to the DTD family. |
Keywords
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Gene Ontology
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| GO:0016788; Molecular function:hydrolase activity, acting on ester bonds |
| GO:0019478; Biological process:D-amino acid catabolic process |
| GO:0051499; Molecular function:D-aminoacyl-tRNA deacylase activity |
| GO:0005737; Cellular component:cytoplasm |
| GO:0000049; Molecular function:tRNA binding |
| GO:0043908; Molecular function:Ser(Gly)-tRNA(Ala) hydrolase activity |
| GO:0106026; Molecular function:Gly-tRNA(Ala) hydrolase activity |
Cross-references
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Features
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| From: DTD_ECOLI (P0A6M4) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 137 | 138 | /note="Gly-cisPro motif, important for rejection of L-amino acids" | motif | G-P | |||||||
Additional information
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| Size range | 127-200 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | BACSU (O32042) is inactive due to missing start codon, SHIF8 (Q0SZ73) may have a sequence error |