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HAMAP rule MF_00518

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General rule information [?]

Accession MF_00518
Dates 17-DEC-2001 (Created)
1-JUN-2023 (Last updated, Version 31)
Name Deacylase_Dtd
Scope(s) Bacteria
Template(s) P0A6M4 (DTD_ECOLI); Q8IIS0 (DTD_PLAF7); [ Recover all ]
Triggered by HAMAP; MF_00518 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DTD
Protein name RecName: Full=D-aminoacyl-tRNA deacylase;
AltName: Full=Gly-tRNA(Ala) deacylase;
Gene name Name=dtd;

Comments [?]

FUNCTIONAn aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA- based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D- aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl- tRNA entities in vivo and helps enforce protein L-homochirality.
CATALYTIC ACTIVITY Reaction=a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+); Xref=Rhea:RHEA:13953, Rhea:RHEA-COMP:10123, Rhea:RHEA-COMP:10124, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:59871, ChEBI:CHEBI:78442, ChEBI:CHEBI:79333; EC=;
CATALYTIC ACTIVITY Reaction=glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala); Xref=Rhea:RHEA:53744, Rhea:RHEA-COMP:9657, Rhea:RHEA-COMP:13640, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57305, ChEBI:CHEBI:78442, ChEBI:CHEBI:78522;
case <FTTag:motif>
DOMAINA Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.
end case
SIMILARITYBelongs to the DTD family.

Keywords [?]

Gene Ontology [?]

GO:0016788; Molecular function:hydrolase activity, acting on ester bonds
GO:0019478; Biological process:D-amino acid catabolic process
GO:0051499; Molecular function:D-aminoacyl-tRNA deacylase activity
GO:0005737; Cellular component:cytoplasm
GO:0000049; Molecular function:tRNA binding
GO:0043908; Molecular function:Ser(Gly)-tRNA(Ala) hydrolase activity
GO:0106026; Molecular function:Gly-tRNA(Ala) hydrolase activity

Cross-references [?]

Pfam PF02580; Tyr_Deacylase; 1;
NCBIfam TIGR00256; TIGR00256; 1;

Features [?]

From: DTD_ECOLI (P0A6M4)
Key From To Description Tag Condition FTGroup
MOTIF 137 138 /note="Gly-cisPro motif, important for rejection of L-amino acids" motif G-P

Additional information [?]

Size range 127-200 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments BACSU (O32042) is inactive due to missing start codon, SHIF8 (Q0SZ73) may have a sequence error

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