HAMAP rule MF_00523
General rule information
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| Accession | MF_00523 |
| Dates | 13-JAN-2002 (Created)
1-JUN-2023 (Last updated, Version 36) |
| Name | LpxD |
| Scope(s) |
Bacteria |
| Template(s) | P21645 (LPXD_ECOLI); P0CD76 (LPXD_CHLTR); [ Recover all ] |
| Triggered by |
HAMAP; MF_00523 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | LPXD |
| case <OC:Enterobacterales> | |
| Protein name | RecName: Full=UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase; Short=UDP-3-O-(3-OHC14)-GlcN N-acyltransferase; EC=2.3.1.191; AltName: Full=UDP-3-O-(3-hydroxytetradecanoyl)glucosamine N-acyltransferase; |
| else | |
| Protein name | RecName: Full=UDP-3-O-acylglucosamine N-acyltransferase; EC=2.3.1.191; |
| end case | |
| Gene name | Name=lpxD; |
Comments
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| case <OC:Enterobacterales> | |
| FUNCTION | Catalyzes the N-acylation of UDP-3-O- (hydroxytetradecanoyl)glucosamine using 3-hydroxytetradecanoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. |
| CATALYTIC ACTIVITY | Reaction=a (3R)-hydroxyacyl-[ACP] + a UDP-3-O-[(3R)-3-hydroxyacyl]- alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D- glucosamine + H(+) + holo-[ACP]; Xref=Rhea:RHEA:53836, Rhea:RHEA- COMP:9685, Rhea:RHEA-COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:64479, ChEBI:CHEBI:78827, ChEBI:CHEBI:137740, ChEBI:CHEBI:137748; EC=2.3.1.191; |
| CATALYTIC ACTIVITY | Reaction=(3R)-hydroxytetradecanoyl-[ACP] + UDP-3-O-[(3R)-3- hydroxytetradecanoyl]-alpha-D-glucosamine = H(+) + holo-[ACP] + UDP- 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine; Xref=Rhea:RHEA:17817, Rhea:RHEA-COMP:9646, Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:64479, ChEBI:CHEBI:71573, ChEBI:CHEBI:78474, ChEBI:CHEBI:78847; |
| PATHWAY | Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 3/6. |
| else | |
| FUNCTION | Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3- hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. |
| CATALYTIC ACTIVITY | Reaction=a (3R)-hydroxyacyl-[ACP] + a UDP-3-O-[(3R)-3-hydroxyacyl]- alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D- glucosamine + H(+) + holo-[ACP]; Xref=Rhea:RHEA:53836, Rhea:RHEA- COMP:9685, Rhea:RHEA-COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:64479, ChEBI:CHEBI:78827, ChEBI:CHEBI:137740, ChEBI:CHEBI:137748; EC=2.3.1.191; |
| PATHWAY | Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. |
| end case | |
| SUBUNIT | Homotrimer. |
| SIMILARITY | Belongs to the transferase hexapeptide repeat family. LpxD subfamily. |
Keywords
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Gene Ontology
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| GO:0016747; Molecular function:acyltransferase activity, transferring groups other than amino-acyl groups |
| GO:0009245; Biological process:lipid A biosynthetic process |
Cross-references
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| Pfam | PF00132; Hexapep; 6-9; |
| Pfam | PF04613; LpxD; 1; |
| NCBIfam | TIGR01853; Lipid_A_lpxD; 1; |
| PROSITE | PS00101; HEXAPEP_TRANSFERASES; 1-4; |
Features
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| From: LPXD_ECOLI (P21645) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 239 | 239 | /note="Proton acceptor" | H | ||||||||
Additional information
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| Size range | 316-380 amino acids |
| Related rules |
MF_00387 |
| Fusion | Nter: None Cter: None |
| Comments | The acyl chain added by LpxD varies depending on the species since there are differences in the structure of lipid A between different species. See Figure 9 from PubMed=19655786, the acyl chain length can vary from C10 to C20. The acyl chain present in the UDP-glucosamine substrate also seems to vary depending on the species (it is UDP-3-O-(hydroxymyristoyl)glucosamine in E.coli and UDP-3-O-myristoylglucosamine in Chlamydia trachomatis). |