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HAMAP rule MF_00523

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General rule information [?]

Accession MF_00523
Dates 13-JAN-2002 (Created)
1-JUN-2023 (Last updated, Version 36)
Name LpxD
Scope
Bacteria
Templates P21645 (LPXD_ECOLI); P0CD76 (LPXD_CHLTR): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
LPXD
case <OC:Enterobacterales>
Protein name
RecName: Full=UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase;
Short=UDP-3-O-(3-OHC14)-GlcN N-acyltransferase;
EC 2.3.1.191;
AltName: Full=UDP-3-O-(3-hydroxytetradecanoyl)glucosamine N-acyltransferase;
else
Protein name
RecName: Full=UDP-3-O-acylglucosamine N-acyltransferase;
EC 2.3.1.191;
end case
Gene name
lpxD

Comments [?]

case <OC:Enterobacterales>
Function Catalyzes the N-acylation of UDP-3-O-(hydroxytetradecanoyl)glucosamine using 3-hydroxytetradecanoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
Catalytic activity RHEA:53836: a (3R)-hydroxyacyl-[ACP] + a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + H(+) + holo-[ACP]
EC 2.3.1.191
RHEA:17817: (3R)-hydroxytetradecanoyl-[ACP] + UDP-3-O-[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine = H(+) + holo-[ACP] + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine
Pathway Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 3/6.
else
Function Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
Catalytic activity RHEA:53836: a (3R)-hydroxyacyl-[ACP] + a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + H(+) + holo-[ACP]
EC 2.3.1.191
Pathway Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.
end case
Subunit Homotrimer.
Similarity Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Keywords [?]

Acyltransferase
Lipid A biosynthesis
Lipid biosynthesis
Lipid metabolism
Repeat
Transferase

Gene Ontology [?]

GO:0016747; Molecular function: acyltransferase activity, transferring groups other than amino-acyl groups.
GO:0009245; Biological process: lipid A biosynthetic process.

Cross-references [?]

Pfam PF00132; Hexapep; 6-9;
PF04613; LpxD; 1;
NCBIfam TIGR01853; Lipid_A_lpxD; 1;
PROSITE PS00101; HEXAPEP_TRANSFERASES; 1-4;

Features [?]

From: LPXD_ECOLI (P21645)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     239     239       Proton acceptor     H  

Additional information [?]

Size range 316-380 amino acids
Related rules MF_00387 (LPXA)
Fusion None
Comments The acyl chain added by LpxD varies depending on the species since there are differences in the structure of lipid A between different species. See Figure 9 from PubMed=19655786, the acyl chain length can vary from C10 to C20. The acyl chain present in the UDP-glucosamine substrate also seems to vary depending on the species (it is UDP-3-O-(hydroxymyristoyl)glucosamine in E.coli and UDP-3-O-myristoylglucosamine in Chlamydia trachomatis).


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