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HAMAP rule MF_00523

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_00523
Accession MF_00523
Dates 28-FEB-2005 (Created)
03-SEP-2024 (Last updated, Version 29)
Name LpxD
Scope(s) Bacteria
Template(s) P21645; P0CD76; [ Recover all ]
Triggered by HAMAP; MF_00523 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier LPXD
case <OC:Enterobacterales>
Protein name RecName: Full=UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase;
                 Short=UDP-3-O-(3-OHC14)-GlcN N-acyltransferase;
                 EC=2.3.1.191;
AltName: Full=UDP-3-O-(3-hydroxytetradecanoyl)glucosamine N-acyltransferase;
else
Protein name RecName: Full=UDP-3-O-acylglucosamine N-acyltransferase;
                 EC=2.3.1.191;
end case
Gene name Name=lpxD;

Comments [?]

case <OC:Enterobacterales>
FUNCTIONCatalyzes the N-acylation of UDP-3-O- (hydroxytetradecanoyl)glucosamine using 3-hydroxytetradecanoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
CATALYTIC ACTIVITY Reaction=a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + a (3R)- hydroxyacyl-[ACP] = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D- glucosamine + holo-[ACP] + H(+); Xref=Rhea:RHEA:53836, Rhea:RHEA- COMP:9685, Rhea:RHEA-COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:64479, ChEBI:CHEBI:78827, ChEBI:CHEBI:137740, ChEBI:CHEBI:137748; EC=2.3.1.191;
CATALYTIC ACTIVITY Reaction=UDP-3-O-[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine + (3R)-hydroxytetradecanoyl-[ACP] = UDP-2-N,3-O-bis[(3R)-3- hydroxytetradecanoyl]-alpha-D-glucosamine + holo-[ACP] + H(+); Xref=Rhea:RHEA:17817, Rhea:RHEA-COMP:9646, Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:64479, ChEBI:CHEBI:71573, ChEBI:CHEBI:78474, ChEBI:CHEBI:78847;
PATHWAYGlycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N- acetyl-alpha-D-glucosamine: step 3/6.
else
FUNCTIONCatalyzes the N-acylation of UDP-3-O-acylglucosamine using 3- hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
CATALYTIC ACTIVITY Reaction=a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + a (3R)- hydroxyacyl-[ACP] = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D- glucosamine + holo-[ACP] + H(+); Xref=Rhea:RHEA:53836, Rhea:RHEA- COMP:9685, Rhea:RHEA-COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:64479, ChEBI:CHEBI:78827, ChEBI:CHEBI:137740, ChEBI:CHEBI:137748; EC=2.3.1.191;
PATHWAYBacterial outer membrane biogenesis; LPS lipid A biosynthesis.
end case
SUBUNITHomotrimer.
SIMILARITYBelongs to the transferase hexapeptide repeat family. LpxD subfamily.

Keywords [?]

Acyltransferase
Lipid A biosynthesis
Lipid biosynthesis
Lipid metabolism
Repeat
Transferase

Gene Ontology [?]

GO:0016747; Molecular function:acyltransferase activity, transferring groups other than amino-acyl groups
GO:0009245; Biological process:lipid A biosynthetic process

Cross-references [?]

Pfam PF00132; Hexapep; 6-9;
Pfam PF04613; LpxD; 1;
NCBIfam TIGR01853; Lipid_A_lpxD; 1;
PROSITE PS00101; HEXAPEP_TRANSFERASES; 1-4;

Features [?]

From: LPXD_ECOLI (P21645)
Key From To Description Tag Condition FTGroup
ACT_SITE 239 239 /note="Proton acceptor" H

Additional information [?]

Size range 316-380 amino acids
Related rules MF_00387
Fusion Nter: None Cter: None
Comments The acyl chain added by LpxD varies depending on the species since there are differences in the structure of lipid A between different species. See Figure 9 from PubMed=19655786, the acyl chain length can vary from C10 to C20. The acyl chain present in the UDP-glucosamine substrate also seems to vary depending on the species (it is UDP-3-O-(hydroxymyristoyl)glucosamine in E.coli and UDP-3-O-myristoylglucosamine in Chlamydia trachomatis).



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