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Annotation rule MF_00528
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General rule information [?]

Accession MF_00528
Dates 18-JAN-2002 (Created)
17-SEP-2020 (Last updated, Version 57)
Name Maf
Scope
Bacteria
Archaea
Templates P25536 (NTPPA_ECOLI); Q02169 (NTPPA_BACSU); P0A729 (NTPPB_ECOLI); P58627 (NTPPB_SALTY); Q99210 (NTPPA_YEAST): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

case <FTGroup:1>
Identifier
NTPPA
Protein name
RecName: Full=dTTP/UTP pyrophosphatase;
Short=dTTPase/UTPase;
EC 3.6.1.9;
AltName: Full=Nucleoside triphosphate pyrophosphatase;
AltName: Full=Nucleotide pyrophosphatase;
Short=Nucleotide PPase;
else case <FTGroup:2>
Identifier
NTPPB
Protein name
RecName: Full=7-methyl-GTP pyrophosphatase;
Short=m(7)GTP pyrophosphatase;
EC 3.6.1.-;
else
Identifier
NTPP
Protein name
RecName: Full=Nucleoside triphosphate pyrophosphatase;
EC 3.6.1.9;
AltName: Full=Nucleotide pyrophosphatase;
Short=Nucleotide PPase;
end case

Comments [?]

case <FTGroup:1>
Function Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.
Catalytic activity RHEA:28534: dTTP + H2O = diphosphate + dTMP + H(+)
EC 3.6.1.9
RHEA:29395: H2O + UTP = diphosphate + H(+) + UMP
EC 3.6.1.9
else case <FTGroup:2>
Function Nucleoside triphosphate pyrophosphatase that hydrolyzes 7-methyl-GTP (m(7)GTP). May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.
Catalytic activity RHEA:58744: H2O + N(7)-methyl-GTP = diphosphate + H(+) + N(7)-methyl-GMP
else
Function Nucleoside triphosphate pyrophosphatase. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.
Catalytic activity RHEA:23996: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H(+)
EC 3.6.1.9
RHEA:44644: a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H(+)
EC 3.6.1.9
end case
Cofactor a divalent metal cation
Subcellular location Cytoplasm.
case <FTGroup:1>
Similarity Belongs to the Maf family. YhdE subfamily.
else case <FTGroup:2>
Similarity Belongs to the Maf family. YceF subfamily.
else
Similarity Belongs to the Maf family.
end case

Keywords [?]


Gene Ontology [?]

GO:0016462; Molecular function: pyrophosphatase activity.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF02545; Maf; 1;
TIGRFAMs TIGR00172; maf; 1;
PIRSF PIRSF006305; Maf; 1;

Features [?]

From: NTPPA_ECOLI (P25536)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     70     70       Proton acceptor     D  
SITE (Optional)     12     12       Important for substrate specificity     R   1
SITE (Optional)     12     12       Important for substrate specificity     [WYF]   2
SITE (Optional)     71     71       Important for substrate specificity     [TS]   1
SITE (Optional)     71     71       Important for substrate specificity     Q   2
SITE (Optional)     153     153       Important for substrate specificity     Q   1
SITE (Optional)     153     153       Important for substrate specificity     E   2

Additional information [?]

Size range 160-350 amino acids
Related rules None
Fusion Nter: None; Cter: <Nudix hydrolase>
Comments Classification into YhdE and YceF subfamilies was done according to PubMed:24210219. Authors define two subfamily-specific motifs: R-T-Q for YhdE and W-Q-E for YceF (see FT SITE). Fusion with a possible Nudix hydrolase in BIFLO.