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Annotation rule MF_00528 |
Accession | MF_00528 |
Dates | 18-JAN-2002 (Created) 17-SEP-2020 (Last updated, Version 57) |
Name | Maf |
Scope | Bacteria
Archaea |
Templates | P25536 (NTPPA_ECOLI); Q02169 (NTPPA_BACSU); P0A729 (NTPPB_ECOLI); P58627 (NTPPB_SALTY); Q99210 (NTPPA_YEAST): [Recover all] |
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Function | Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids. |
Catalytic activity | RHEA:28534: dTTP + H2O = diphosphate + dTMP + H(+)
EC 3.6.1.9 |
RHEA:29395: H2O + UTP = diphosphate + H(+) + UMP
EC 3.6.1.9 |
Function | Nucleoside triphosphate pyrophosphatase that hydrolyzes 7-methyl-GTP (m(7)GTP). May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids. |
Catalytic activity | RHEA:58744: H2O + N(7)-methyl-GTP = diphosphate + H(+) + N(7)-methyl-GMP |
Function | Nucleoside triphosphate pyrophosphatase. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids. |
Catalytic activity | RHEA:23996: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H(+)
EC 3.6.1.9 |
RHEA:44644: a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H(+)
EC 3.6.1.9 |
Cofactor | a divalent metal cation |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the Maf family. YhdE subfamily. |
Similarity | Belongs to the Maf family. YceF subfamily. |
Similarity | Belongs to the Maf family. |
Pfam | PF02545; Maf; 1; |
TIGRFAMs | TIGR00172; maf; 1; |
PIRSF | PIRSF006305; Maf; 1; |
From: NTPPA_ECOLI (P25536) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 70 | 70 | Proton acceptor | D | ||||||||
SITE (Optional) | 12 | 12 | Important for substrate specificity | R | 1 | |||||||
SITE (Optional) | 12 | 12 | Important for substrate specificity | [WYF] | 2 | |||||||
SITE (Optional) | 71 | 71 | Important for substrate specificity | [TS] | 1 | |||||||
SITE (Optional) | 71 | 71 | Important for substrate specificity | Q | 2 | |||||||
SITE (Optional) | 153 | 153 | Important for substrate specificity | Q | 1 | |||||||
SITE (Optional) | 153 | 153 | Important for substrate specificity | E | 2 |
Size range | 160-350 amino acids |
Related rules | None |
Fusion | Nter: None; Cter: <Nudix hydrolase> |
Comments | Classification into YhdE and YceF subfamilies was done according to PubMed:24210219. Authors define two subfamily-specific motifs: R-T-Q for YhdE and W-Q-E for YceF (see FT SITE). Fusion with a possible Nudix hydrolase in BIFLO. |