HAMAP rule MF_00533
General rule information
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| Accession | MF_00533 |
| Dates | 21-JAN-2002 (Created)
1-JUN-2023 (Last updated, Version 47) |
| Name | NifH |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P00459 (NIFH1_AZOVI); P00456 (NIFH1_CLOPA); [ Recover all ] |
| Triggered by |
HAMAP; MF_00533 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NIFH |
| Protein name | RecName: Full=Nitrogenase iron protein; EC=1.18.6.1; AltName: Full=Nitrogenase Fe protein; AltName: Full=Nitrogenase component II; AltName: Full=Nitrogenase reductase; |
| Gene name | Name=nifH; |
Comments
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| FUNCTION | The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein. |
| CATALYTIC ACTIVITY | Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA- COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997, ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=1.18.6.1; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per dimer.; |
| SUBUNIT | Homodimer. |
| case <FT:4> | |
| PTM | The reversible ADP-ribosylation of #{Arg-101} inactivates the nitrogenase reductase and regulates nitrogenase activity. |
| end case | |
| SIMILARITY | Belongs to the NifH/BchL/ChlL family. |
Keywords
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| Oxidoreductase | |
| Nitrogen fixation | |
| Metal-binding | |
| Iron | |
| Iron-sulfur | |
| 4Fe-4S | |
| ATP-binding | |
| Nucleotide-binding | |
| case <FT:4> | |
| ADP-ribosylation | |
| end case | |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0016163; Molecular function:nitrogenase activity |
| GO:0009399; Biological process:nitrogen fixation |
Cross-references
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| Pfam | PF00142; Fer4_NifH; 1; |
| PIRSF | PIRSF000363; Nitrogenase_iron; 1; |
| PRINTS | PR00091; NITROGNASEII; 1; |
| NCBIfam | TIGR01287; NifH; 1; |
| PROSITE | PS00746; NIFH_FRXC_1; 1; |
| PROSITE | PS00692; NIFH_FRXC_2; 1; |
| PROSITE | PS51026; NIFH_FRXC_3; 1; |
Features
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| From: NIFH1_AZOVI (P00459) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 10 | 17 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-K-G-G-I-[GA]-K-S | ||||||||
| BINDING | 98 | 98 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="ligand shared between dimeric partners" |
C | ||||||||
| BINDING | 133 | 133 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="ligand shared between dimeric partners" |
C | ||||||||
| MOD_RES | 101 | 101 | /note="ADP-ribosylarginine; by dinitrogenase reductase ADP- ribosyltransferase" | R | ||||||||
Additional information
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| Size range | 263-307 amino acids |
| Related rules |
MF_00355 |
| Fusion | Nter: None Cter: None |
| Comments | CROS5 has a divergent nifH with a 37 N-terminal extension; this sequence is not shown in the alignment and not used for the size range |