HAMAP rule MF_00533
General rule information
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| Accession | MF_00533 |
| Dates | 21-JAN-2002 (Created) 1-JUN-2023 (Last updated, Version 47) |
| Name | NifH |
| Scope | Bacteria
Archaea |
| Templates | P00459 (NIFH1_AZOVI); P00456 (NIFH1_CLOPA): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein. |
| Catalytic activity | RHEA:21448: 16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate
EC 1.18.6.1 |
| Cofactor | [4Fe-4S] cluster Note: Binds 1 [4Fe-4S] cluster per dimer. |
| Subunit | Homodimer. |
case <FT:4>
| Ptm | The reversible ADP-ribosylation of #{Arg-101} inactivates the nitrogenase reductase and regulates nitrogenase activity. |
end case
| Similarity | Belongs to the NifH/BchL/ChlL family. |
Keywords
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Oxidoreductase
Nitrogen fixation
Metal-binding
Iron
Iron-sulfur
4Fe-4S
ATP-binding
Nucleotide-binding
Nitrogen fixation
Metal-binding
Iron
Iron-sulfur
4Fe-4S
ATP-binding
Nucleotide-binding
case <FT:4>
end case
Gene Ontology
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GO:0005524; Molecular function: ATP binding.
GO:0016163; Molecular function: nitrogenase activity.
GO:0009399; Biological process: nitrogen fixation.
GO:0016163; Molecular function: nitrogenase activity.
GO:0009399; Biological process: nitrogen fixation.
Cross-references
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| Pfam | PF00142; Fer4_NifH; 1; |
| PIRSF | PIRSF000363; Nitrogenase_iron; 1; |
| PRINTS | PR00091; NITROGNASEII; 1; |
| NCBIfam | TIGR01287; NifH; 1; |
| PROSITE | PS00746; NIFH_FRXC_1; 1; |
| PS00692; NIFH_FRXC_2; 1; | |
| PS51026; NIFH_FRXC_3; 1; |
Features
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| From: NIFH1_AZOVI (P00459) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 10 | 17 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G-K-G-G-I-[GA]-K-S | ||||||||
| BINDING | 98 | 98 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="ligand shared between dimeric partners | C | ||||||||
| BINDING | 133 | 133 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="ligand shared between dimeric partners | C | ||||||||
| MOD_RES | 101 | 101 | ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase | R | ||||||||
Additional information
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| Size range | 263-307 amino acids |
| Related rules | MF_00355 (BCHL) |
| Fusion | None |
| Comments | CROS5 has a divergent nifH with a 37 N-terminal extension; this sequence is not shown in the alignment and not used for the size range |