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Annotation rule MF_00533
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General rule information [?]

Accession MF_00533
Dates 21-JAN-2002 (Created)
18-NOV-2019 (Last updated, Version 43)
Name NifH
Scope
Bacteria
Archaea
Templates P00459 (NIFH1_AZOVI); P00456 (NIFH1_CLOPA): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NIFH
Protein name
RecName: Full=Nitrogenase iron protein;
EC 1.18.6.1;
AltName: Full=Nitrogenase Fe protein;
AltName: Full=Nitrogenase component II;
AltName: Full=Nitrogenase reductase;
Gene name
nifH

Comments [?]

Function The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.
Catalytic activity RHEA:21448: 16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate
EC 1.18.6.1
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster per dimer.
Subunit Homodimer.
case <FT:4>
Ptm The reversible ADP-ribosylation of #{Arg-101} inactivates the nitrogenase reductase and regulates nitrogenase activity.
end case
Similarity Belongs to the NifH/BchL/ChlL family.

Keywords [?]

case <FT:4>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0016163; Molecular function: nitrogenase activity.
GO:0009399; Biological process: nitrogen fixation.
GO:0055114; Biological process: oxidation-reduction process.

Cross-references [?]

Pfam PF00142; Fer4_NifH; 1;
PIRSF PIRSF000363; Nitrogenase_iron; 1;
PRINTS PR00091; NITROGNASEII; 1;
TIGRFAMs TIGR01287; NifH; 1;
PROSITE PS00746; NIFH_FRXC_1; 1;
PS00692; NIFH_FRXC_2; 1;
PS51026; NIFH_FRXC_3; 1;

Features [?]

From: NIFH1_AZOVI (P00459)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     10     17       ATP     G-K-G-G-I-[GA]-K-S  
METAL     98     98       Iron-sulfur (4Fe-4S); shared with dimeric partner     C  
METAL     133     133       Iron-sulfur (4Fe-4S); shared with dimeric partner     C  
MOD_RES     101     101       ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase     R  

Additional information [?]

Size range 263-307 amino acids
Related rules MF_00355 (BCHL)
Fusion None
Comments CROS5 has a divergent nifH with a 37 N-terminal extension; this sequence is not shown in the alignment and not used for the size range