HAMAP rule MF_00553

General rule information [?]

Accession	MF_00553
Dates	9-MAY-2002 (Created) 1-JUN-2023 (Last updated, Version 31)

Name

PAN

Scope

Archaea

Templates

Q58576 (PAN_METJA); O28303 (PAN_ARCFU); D4GUJ7 (PAN1_HALVD); Q5UT56 (PAN2_HALVD): [Recover all]

Triggered by

HAMAP; MF_00553 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PAN

Protein name

RecName:		Full=Proteasome-activating nucleotidase;
		Short=PAN;
AltName:		Full=Proteasomal ATPase;
AltName:		Full=Proteasome regulatory ATPase;
AltName:		Full=Proteasome regulatory particle;

Gene name

pan

Comments [?]

Function	ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.
Subunit	Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.
Subcellular location	Cytoplasm.
Domain	Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.
Similarity	Belongs to the AAA ATPase family.

Keywords [?]

Chaperone
Cytoplasm
Proteasome
ATP-binding
Nucleotide-binding

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0016887; Molecular function: ATP hydrolysis activity.
GO:0010498; Biological process: proteasomal protein catabolic process.
GO:0043335; Biological process: protein unfolding.
GO:0000502; Cellular component: proteasome complex.
GO:0005737; Cellular component: cytoplasm.
GO:0022623; Cellular component: proteasome-activating nucleotidase complex.

Cross-references [?]

PROSITE	PS00674; AAA; 1;
Pfam	PF00004; AAA; 1;
NCBIfam	TIGR01242; 26Sp45; 1;

Computed features [?]

General

Coiled_coil; -; 0-unlimited; trigger=yes;

Features [?]

From: PAN_METJA (Q58576)

Key From To Description Tag Condition FTGroup

BINDING 214 219 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-x-G-K-T-[LM]

REGION 428 430 Docks into pockets in the proteasome alpha-ring to cause gate opening x-[YF]-x

BINDING 353 353 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [HY]

Additional information [?]

Size range	392-436 amino acids
Related rules	None
Fusion	None
Comments	The protein performing the same function in bacteria (called ARC) is in MF_02112.

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