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HAMAP rule MF_00553

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General rule information [?]

Accession MF_00553
Dates 9-MAY-2002 (Created)
1-JUN-2023 (Last updated, Version 31)
Name PAN
Templates Q58576 (PAN_METJA); O28303 (PAN_ARCFU); D4GUJ7 (PAN1_HALVD); Q5UT56 (PAN2_HALVD): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Proteasome-activating nucleotidase;
AltName: Full=Proteasomal ATPase;
AltName: Full=Proteasome regulatory ATPase;
AltName: Full=Proteasome regulatory particle;
Gene name

Comments [?]

Function ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.
Subunit Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.
Subcellular location Cytoplasm.
Domain Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.
Similarity Belongs to the AAA ATPase family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0016887; Molecular function: ATP hydrolysis activity.
GO:0010498; Biological process: proteasomal protein catabolic process.
GO:0043335; Biological process: protein unfolding.
GO:0000502; Cellular component: proteasome complex.
GO:0005737; Cellular component: cytoplasm.
GO:0022623; Cellular component: proteasome-activating nucleotidase complex.

Cross-references [?]

PROSITE PS00674; AAA; 1;
Pfam PF00004; AAA; 1;
NCBIfam TIGR01242; 26Sp45; 1;

Computed features [?]

General Coiled_coil; -; 0-unlimited; trigger=yes;

Features [?]

From: PAN_METJA (Q58576)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     214     219       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-x-G-K-T-[LM]  
REGION     428     430       Docks into pockets in the proteasome alpha-ring to cause gate opening     x-[YF]-x  
BINDING     353     353       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [HY]  

Additional information [?]

Size range 392-436 amino acids
Related rules None
Fusion None
Comments The protein performing the same function in bacteria (called ARC) is in MF_02112.