HAMAP rule MF_00554
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00554 |
| Accession | MF_00554 |
| Dates | 9-MAY-2002 (Created)
3-SEP-2024 (Last updated, Version 30) |
| Name | NEP1 |
| Scope(s) |
Archaea |
| Template(s) | Q57977 (NEP1_METJA); Q06287 (NEP1_YEAST); [ Recover all ] |
| Triggered by |
HAMAP; MF_00554 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NEP1 |
| Protein name | RecName: Full=Ribosomal RNA small subunit methyltransferase Nep1; EC=2.1.1.-; AltName: Full=16S rRNA (pseudouridine-N1-)-methyltransferase Nep1; |
| Gene name | Name=nep1; |
Comments
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| FUNCTION | Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. |
| CATALYTIC ACTIVITY | Reaction=a pseudouridine in rRNA + S-adenosyl-L-methionine = an N(1)- methylpseudouridine in rRNA + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:46696, Rhea:RHEA-COMP:11634, Rhea:RHEA-COMP:13933, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65314, ChEBI:CHEBI:74890; |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family. |
Keywords
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| Methyltransferase |
| Ribosome biogenesis |
| RNA-binding |
| rRNA-binding |
| rRNA processing |
| S-adenosyl-L-methionine |
| Transferase |
Gene Ontology
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| GO:0019843; Molecular function:rRNA binding |
| GO:0070037; Molecular function:rRNA (pseudouridine) methyltransferase activity |
| GO:0042254; Biological process:ribosome biogenesis |
| GO:0006364; Biological process:rRNA processing |
Cross-references
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| Pfam | PF03587; EMG1; 1; |
Features
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| From: NEP1_METJA (Q57977) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 185 | 190 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
|||||||||
| BINDING | 162 | 162 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | ||||||||
| BINDING | 167 | 167 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | ||||||||
| SITE | 54 | 54 | /note="Interaction with substrate rRNA" | R | ||||||||
| SITE | 56 | 56 | /note="Stabilizes Arg-xx" | D | ||||||||
| SITE | 95 | 95 | /note="Interaction with substrate rRNA" | R | ||||||||
| SITE | 98 | 98 | /note="Interaction with substrate rRNA" | [RK] | ||||||||
| SITE | 102 | 102 | /note="Interaction with substrate rRNA" | [RN] | ||||||||
Additional information
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| Size range | 205-232 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |