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HAMAP rule MF_00554

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General rule information [?]

Accession MF_00554
Dates 9-MAY-2002 (Created)
22-FEB-2024 (Last updated, Version 29)
Name NEP1
Scope(s) Archaea
Template(s) Q57977 (NEP1_METJA); Q06287 (NEP1_YEAST); [ Recover all ]
Triggered by HAMAP; MF_00554 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NEP1
Protein name RecName: Full=Ribosomal RNA small subunit methyltransferase Nep1;
AltName: Full=16S rRNA (pseudouridine-N1-)-methyltransferase Nep1;
Gene name Name=nep1;

Comments [?]

FUNCTIONMethyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.
CATALYTIC ACTIVITY Reaction=a pseudouridine in rRNA + S-adenosyl-L-methionine = an N(1)- methylpseudouridine in rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:46696, Rhea:RHEA-COMP:11634, Rhea:RHEA-COMP:13933, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65314, ChEBI:CHEBI:74890;
SIMILARITYBelongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.

Keywords [?]

Gene Ontology [?]

GO:0019843; Molecular function:rRNA binding
GO:0070037; Molecular function:rRNA (pseudouridine) methyltransferase activity
GO:0042254; Biological process:ribosome biogenesis
GO:0006364; Biological process:rRNA processing

Cross-references [?]

Pfam PF03587; EMG1; 1;

Features [?]

From: NEP1_METJA (Q57977)
Key From To Description Tag Condition FTGroup
BINDING 185 190 /ligand="S-adenosyl-L-methionine"
BINDING 162 162 /ligand="S-adenosyl-L-methionine"
BINDING 167 167 /ligand="S-adenosyl-L-methionine"
SITE 54 54 /note="Interaction with substrate rRNA" R
SITE 56 56 /note="Stabilizes Arg-xx" D
SITE 95 95 /note="Interaction with substrate rRNA" R
SITE 98 98 /note="Interaction with substrate rRNA" [RK]
SITE 102 102 /note="Interaction with substrate rRNA" [RN]

Additional information [?]

Size range 205-232 amino acids
Related rules None
Fusion Nter: None Cter: None

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