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HAMAP rule MF_00554

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General rule information [?]

Accession MF_00554
Dates 9-MAY-2002 (Created)
19-NOV-2022 (Last updated, Version 28)
Name NEP1
Templates Q57977 (NEP1_METJA); Q06287 (NEP1_YEAST): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Ribosomal RNA small subunit methyltransferase Nep1;
EC 2.1.1.-;
AltName: Full=16S rRNA (pseudouridine-N1-)-methyltransferase Nep1;
Gene name

Comments [?]

Function Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.
Catalytic activity RHEA:46696: a pseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in 16S/18S rRNA + H(+) + S-adenosyl-L-homocysteine
Subunit Homodimer.
Similarity Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.

Keywords [?]

Gene Ontology [?]

GO:0019843; Molecular function: rRNA binding.
GO:0070037; Molecular function: rRNA (pseudouridine) methyltransferase activity.
GO:0042254; Biological process: ribosome biogenesis.
GO:0006364; Biological process: rRNA processing.

Cross-references [?]

Pfam PF03587; EMG1; 1;

Features [?]

From: NEP1_METJA (Q57977)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     185     190       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789        
BINDING     162     162       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     G  
BINDING     167     167       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     G  
SITE     54     54       Interaction with substrate rRNA     R  
SITE     56     56       Stabilizes Arg-xx     D  
SITE     95     95       Interaction with substrate rRNA     R  
SITE     98     98       Interaction with substrate rRNA     [RK]  
SITE     102     102       Interaction with substrate rRNA     [RN]  

Additional information [?]

Size range 205-232 amino acids
Related rules None
Fusion None