HAMAP rule MF_00554

General rule information [?]

Accession	MF_00554
Dates	9-MAY-2002 (Created) 19-NOV-2022 (Last updated, Version 28)

Name

NEP1

Scope

Archaea

Templates

Q57977 (NEP1_METJA); Q06287 (NEP1_YEAST): [Recover all]

Triggered by

HAMAP; MF_00554 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

NEP1

Protein name

RecName:		Full=Ribosomal RNA small subunit methyltransferase Nep1;
		EC 2.1.1.-;
AltName:		Full=16S rRNA (pseudouridine-N1-)-methyltransferase Nep1;

Gene name

nep1

Comments [?]

Function	Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.
Catalytic activity	RHEA:46696: a pseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in 16S/18S rRNA + H(+) + S-adenosyl-L-homocysteine
Subunit	Homodimer.
Similarity	Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.

Keywords [?]

Methyltransferase
Ribosome biogenesis
RNA-binding
rRNA-binding
rRNA processing
S-adenosyl-L-methionine
Transferase

Gene Ontology [?]

GO:0019843; Molecular function: rRNA binding.
GO:0070037; Molecular function: rRNA (pseudouridine) methyltransferase activity.
GO:0042254; Biological process: ribosome biogenesis.
GO:0006364; Biological process: rRNA processing.

Cross-references [?]

Pfam	PF03587; EMG1; 1;

Features [?]

From: NEP1_METJA (Q57977)

Key From To Description Tag Condition FTGroup

BINDING 185 190 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789

BINDING 162 162 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 G

BINDING 167 167 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 G

SITE 54 54 Interaction with substrate rRNA R

SITE 56 56 Stabilizes Arg-xx D

SITE 95 95 Interaction with substrate rRNA R

SITE 98 98 Interaction with substrate rRNA [RK]

SITE 102 102 Interaction with substrate rRNA [RN]

Additional information [?]

Size range	205-232 amino acids
Related rules	None
Fusion	None

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