HAMAP rule MF_00554
General rule information
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Accession | MF_00554 |
Dates | 9-MAY-2002 (Created)
19-NOV-2022 (Last updated, Version 28) |
Name | NEP1 |
Scope(s) |
Archaea |
Template(s) | Q57977 (NEP1_METJA); Q06287 (NEP1_YEAST); [ Recover all ] |
Triggered by |
HAMAP; MF_00554 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | NEP1 |
Protein name | RecName: Full=Ribosomal RNA small subunit methyltransferase Nep1; EC=2.1.1.-; AltName: Full=16S rRNA (pseudouridine-N1-)-methyltransferase Nep1; |
Gene name | Name=nep1; |
Comments
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FUNCTION | Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. |
CATALYTIC ACTIVITY | Reaction=a pseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in 16S/18S rRNA + H(+) + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:46696, Rhea:RHEA-COMP:11633, Rhea:RHEA- COMP:11634, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65314, ChEBI:CHEBI:74890; |
SUBUNIT | Homodimer. |
SIMILARITY | Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family. |
Keywords
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Methyltransferase |
Ribosome biogenesis |
RNA-binding |
rRNA-binding |
rRNA processing |
S-adenosyl-L-methionine |
Transferase |
Gene Ontology
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GO:0019843; Molecular function:rRNA binding |
GO:0070037; Molecular function:rRNA (pseudouridine) methyltransferase activity |
GO:0042254; Biological process:ribosome biogenesis |
GO:0006364; Biological process:rRNA processing |
Cross-references
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Pfam | PF03587; EMG1; 1; |
Features
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From: NEP1_METJA (Q57977) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 185 | 190 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
|||||||||
BINDING | 162 | 162 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | ||||||||
BINDING | 167 | 167 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | ||||||||
SITE | 54 | 54 | /note="Interaction with substrate rRNA" | R | ||||||||
SITE | 56 | 56 | /note="Stabilizes Arg-xx" | D | ||||||||
SITE | 95 | 95 | /note="Interaction with substrate rRNA" | R | ||||||||
SITE | 98 | 98 | /note="Interaction with substrate rRNA" | [RK] | ||||||||
SITE | 102 | 102 | /note="Interaction with substrate rRNA" | [RN] |
Additional information
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Size range | 205-232 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |