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HAMAP rule MF_00564
General rule information
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| Accession | MF_00564 |
| Dates | 27-JUN-2002 (Created)
27-JUL-2023 (Last updated, Version 30) |
| Name | RNase_PH |
| Scope(s) |
Bacteria |
| Template(s) | P28619 (RNPH_BACSU); P0CG18 (RNPH_ECOBW); P0CG19 (RNPH_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00564 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RNPH |
| Protein name | RecName: Full=Ribonuclease PH; Short=RNase PH; EC=2.7.7.56; AltName: Full=tRNA nucleotidyltransferase; |
| Gene name | Name=rph; |
Comments
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| FUNCTION | Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. |
| CATALYTIC ACTIVITY | Reaction=phosphate + tRNA(n+1) = a ribonucleoside 5'-diphosphate + tRNA(n); Xref=Rhea:RHEA:10628, Rhea:RHEA-COMP:17343, Rhea:RHEA- COMP:17344, ChEBI:CHEBI:43474, ChEBI:CHEBI:57930, ChEBI:CHEBI:173114; EC=2.7.7.56; |
| SUBUNIT | Homohexameric ring arranged as a trimer of dimers. |
| SIMILARITY | Belongs to the RNase PH family. |
Keywords
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Gene Ontology
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| GO:0000049; Molecular function:tRNA binding |
| GO:0009022; Molecular function:tRNA nucleotidyltransferase activity |
| GO:0008033; Biological process:tRNA processing |
| GO:0000175; Molecular function:3'-5'-RNA exonuclease activity |
| GO:0016075; Biological process:rRNA catabolic process |
Cross-references
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| Pfam | PF01138; RNase_PH; 1; |
| Pfam | PF03725; RNase_PH_C; 1; |
| PROSITE | PS01277; RIBONUCLEASE_PH; 1; |
| NCBIfam | TIGR01966; RNasePH; 1; |
Features
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| From: RNPH_PSEAE (P50597) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 125 | 127 | /ligand="phosphate" /ligand_id="ChEBI:CHEBI:43474" /ligand_note="substrate" |
[GS]-T-R | ||||||||
| BINDING | 87 | 87 | /ligand="phosphate" /ligand_id="ChEBI:CHEBI:43474" /ligand_note="substrate" |
R | ||||||||
Additional information
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| Size range | 221-261 amino acids |
| Related rules |
MF_00591 MF_00622 |
| Fusion | Nter: None Cter: None |