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HAMAP rule MF_00564

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General rule information [?]

Accession MF_00564
Dates 27-JUN-2002 (Created)
27-JUL-2023 (Last updated, Version 30)
Name RNase_PH
Scope(s) Bacteria
Template(s) P28619 (RNPH_BACSU); P0CG18 (RNPH_ECOBW); P0CG19 (RNPH_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00564 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RNPH
Protein name RecName: Full=Ribonuclease PH;
                 Short=RNase PH;
AltName: Full=tRNA nucleotidyltransferase;
Gene name Name=rph;

Comments [?]

FUNCTIONPhosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.
CATALYTIC ACTIVITY Reaction=phosphate + tRNA(n+1) = a ribonucleoside 5'-diphosphate + tRNA(n); Xref=Rhea:RHEA:10628, Rhea:RHEA-COMP:17343, Rhea:RHEA- COMP:17344, ChEBI:CHEBI:43474, ChEBI:CHEBI:57930, ChEBI:CHEBI:173114; EC=;
SUBUNITHomohexameric ring arranged as a trimer of dimers.
SIMILARITYBelongs to the RNase PH family.

Keywords [?]

Gene Ontology [?]

GO:0000049; Molecular function:tRNA binding
GO:0009022; Molecular function:tRNA nucleotidyltransferase activity
GO:0008033; Biological process:tRNA processing
GO:0000175; Molecular function:3'-5'-RNA exonuclease activity
GO:0016075; Biological process:rRNA catabolic process

Cross-references [?]

Pfam PF01138; RNase_PH; 1;
Pfam PF03725; RNase_PH_C; 1;
NCBIfam TIGR01966; RNasePH; 1;

Features [?]

From: RNPH_PSEAE (P50597)
Key From To Description Tag Condition FTGroup
BINDING 125 127 /ligand="phosphate"
BINDING 87 87 /ligand="phosphate"

Additional information [?]

Size range 221-261 amino acids
Related rules MF_00591
Fusion Nter: None Cter: None

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