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HAMAP rule MF_00572

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General rule information [?]

Accession MF_00572
Dates 12-JUL-2002 (Created)
1-DEC-2022 (Last updated, Version 31)
Name LeuA_type2
Scope
Bacteria
Templates P42455 (LEU1_CORGL); P9WQB3 (LEU1_MYCTU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
LEU1
Protein name
RecName: Full=2-isopropylmalate synthase;
EC 2.3.3.13;
AltName: Full=Alpha-IPM synthase;
AltName: Full=Alpha-isopropylmalate synthase;
Gene name
leuA

Comments [?]

Function Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
Catalytic activity RHEA:21524: 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)
EC 2.3.3.13
case <FTGroup:1>
Cofactor Mg(2+)
end case
Pathway Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.

Keywords [?]

Amino-acid biosynthesis
Branched-chain amino acid biosynthesis
Cytoplasm
Leucine biosynthesis
Transferase
case <FTGroup:1>
Magnesium
Metal-binding
end case

Gene Ontology [?]

GO:0005737; Cellular component: cytoplasm.
GO:0003852; Molecular function: 2-isopropylmalate synthase activity.
GO:0003985; Molecular function: acetyl-CoA C-acetyltransferase activity.
GO:0009098; Biological process: leucine biosynthetic process.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case

Cross-references [?]

Pfam PF00682; HMGL-like; 1;
PF08502; LeuA_dimer; 1;
TIGRFAMs TIGR00970; leuA_yeast; 1;
PROSITE PS00815; AIPM_HOMOCIT_SYNTH_1; 1;
PS00816; AIPM_HOMOCIT_SYNTH_2; 1;
PS50991; PYR_CT; 1; trigger=PRU01151;

Features [?]

From: LEU1_MYCTU (P9WQB3)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     491     Cter       Regulatory domain        
BINDING     81     81       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D   1
BINDING     285     285       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     H   1
BINDING     287     287       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     H   1
BINDING     321     321       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     N   1

Additional information [?]

Size range 530-675 amino acids
Related rules MF_01025 (LEU1); MF_01028 (CIMA)
Fusion None
Comments Some Mycobacteria have variable number tandem repeats (VNTR) insertions in this gene; for example M.tuberculosis strains H37Rv and CDC 1551 have 2 repeats, M.bovis strain ATCC BAA-935 / AF2122/97 has 5 repeats.


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