HAMAP rule MF_00572
General rule information
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Accession | MF_00572 |
Dates | 12-JUL-2002 (Created) 1-DEC-2022 (Last updated, Version 31) |
Name | LeuA_type2 |
Scope | Bacteria |
Templates | P42455 (LEU1_CORGL); P9WQB3 (LEU1_MYCTU): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). |
Catalytic activity | RHEA:21524: 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)
EC 2.3.3.13 |
case <FTGroup:1>
Cofactor | Mg(2+) |
end case
Pathway | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. |
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. |
Keywords
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Amino-acid biosynthesis
Branched-chain amino acid biosynthesis
Cytoplasm
Leucine biosynthesis
Transferase
Branched-chain amino acid biosynthesis
Cytoplasm
Leucine biosynthesis
Transferase
case <FTGroup:1>
end case
Gene Ontology
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GO:0005737; Cellular component: cytoplasm.
GO:0003852; Molecular function: 2-isopropylmalate synthase activity.
GO:0003985; Molecular function: acetyl-CoA C-acetyltransferase activity.
GO:0009098; Biological process: leucine biosynthetic process.
GO:0003852; Molecular function: 2-isopropylmalate synthase activity.
GO:0003985; Molecular function: acetyl-CoA C-acetyltransferase activity.
GO:0009098; Biological process: leucine biosynthetic process.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
Cross-references
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Pfam | PF00682; HMGL-like; 1; |
PF08502; LeuA_dimer; 1; | |
TIGRFAMs | TIGR00970; leuA_yeast; 1; |
PROSITE | PS00815; AIPM_HOMOCIT_SYNTH_1; 1; |
PS00816; AIPM_HOMOCIT_SYNTH_2; 1; | |
PS50991; PYR_CT; 1; trigger=PRU01151; |
Features
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From: LEU1_MYCTU (P9WQB3) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 491 | Cter | Regulatory domain | |||||||||
BINDING | 81 | 81 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | 1 | |||||||
BINDING | 285 | 285 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | H | 1 | |||||||
BINDING | 287 | 287 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | H | 1 | |||||||
BINDING | 321 | 321 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | N | 1 |
Additional information
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Size range | 530-675 amino acids |
Related rules | MF_01025 (LEU1); MF_01028 (CIMA) |
Fusion | None |
Comments | Some Mycobacteria have variable number tandem repeats (VNTR) insertions in this gene; for example M.tuberculosis strains H37Rv and CDC 1551 have 2 repeats, M.bovis strain ATCC BAA-935 / AF2122/97 has 5 repeats. |