HAMAP rule MF_00581
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00581 |
| Accession | MF_00581 |
| Dates | 28-FEB-2005 (Created)
05-NOV-2024 (Last updated, Version 34) |
| Name | Arg_succ_synth_type2 |
| Scope(s) |
Bacteria |
| Template(s) | P0A6E4; [ Recover all ] |
| Triggered by |
HAMAP; MF_00581 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ASSY |
| Protein name | RecName: Full=Argininosuccinate synthase; EC=6.3.4.5; AltName: Full=Citrulline--aspartate ligase; |
| Gene name | Name=argG; |
Comments
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| CATALYTIC ACTIVITY | Reaction=L-citrulline + L-aspartate + ATP = 2-(N(omega)-L- arginino)succinate + AMP + diphosphate + H(+); Xref=Rhea:RHEA:10932, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57472, ChEBI:CHEBI:57743, ChEBI:CHEBI:456215; EC=6.3.4.5; |
| PATHWAY | Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. |
| SUBUNIT | Homotetramer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the argininosuccinate synthase family. Type 2 subfamily. |
Keywords
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Gene Ontology
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| GO:0004055; Molecular function:argininosuccinate synthase activity |
| GO:0005524; Molecular function:ATP binding |
| GO:0006526; Biological process:L-arginine biosynthetic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00764; Arginosuc_synth; 1; |
| NCBIfam | TIGR00032; ArgG; 1; |
| PROSITE | PS00564; ARGININOSUCCIN_SYN_1; 1; |
| PROSITE | PS00565; ARGININOSUCCIN_SYN_2; 1; |
Features
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| From: ASSY_ECOLI (P0A6E4) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 17 | 25 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
|||||||||
| BINDING | 43 | 43 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
A | ||||||||
| BINDING | 99 | 99 | /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743" |
Y | ||||||||
| BINDING | 129 | 129 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| BINDING | 131 | 131 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
T | ||||||||
| BINDING | 131 | 131 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991" |
T | ||||||||
| BINDING | 135 | 135 | /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743" |
N | ||||||||
| BINDING | 135 | 135 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991" |
N | ||||||||
| BINDING | 136 | 136 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
D | ||||||||
| BINDING | 136 | 136 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991" |
D | ||||||||
| BINDING | 139 | 139 | /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743" |
R | ||||||||
| BINDING | 192 | 192 | /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743" |
S | ||||||||
| BINDING | 194 | 194 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
D | ||||||||
| BINDING | 201 | 201 | /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743" |
T | ||||||||
| BINDING | 203 | 203 | /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743" |
E | ||||||||
| BINDING | 280 | 280 | /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743" |
E | ||||||||
Additional information
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| Size range | 444-482 amino acids |
| Related rules |
MF_00005 |
| Fusion | Nter: None Cter: None |
| Comments | Argininosuccinate synthase type 1 subfamily in MF_00005. |