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HAMAP rule MF_00581

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General rule information [?]

Accession MF_00581
Dates 17-OCT-2002 (Created)
1-JUN-2023 (Last updated, Version 40)
Name Arg_succ_synth_type2
Scope
Bacteria
Template P0A6E4 (ASSY_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ASSY
Protein name
RecName: Full=Argininosuccinate synthase;
EC 6.3.4.5;
AltName: Full=Citrulline--aspartate ligase;
Gene name
argG

Comments [?]

Catalytic activity RHEA:10932: ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)
EC 6.3.4.5
Pathway Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
Subunit Homotetramer.
Subcellular location Cytoplasm.
Similarity Belongs to the argininosuccinate synthase family. Type 2 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004055; Molecular function: argininosuccinate synthase activity.
GO:0005524; Molecular function: ATP binding.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00764; Arginosuc_synth; 1;
NCBIfam TIGR00032; ArgG; 1;
PROSITE PS00564; ARGININOSUCCIN_SYN_1; 1;
PS00565; ARGININOSUCCIN_SYN_2; 1;

Features [?]

From: ASSY_ECOLI (P0A6E4)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     17     25       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616        
BINDING     43     43       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     A  
BINDING     99     99       /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743     Y  
BINDING     129     129       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G  
BINDING     131     131       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     T  
BINDING     131     131       /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991     T  
BINDING     135     135       /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743     N  
BINDING     135     135       /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991     N  
BINDING     136     136       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     D  
BINDING     136     136       /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991     D  
BINDING     139     139       /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743     R  
BINDING     192     192       /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743     S  
BINDING     194     194       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     D  
BINDING     201     201       /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743     T  
BINDING     203     203       /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743     E  
BINDING     280     280       /ligand="L-citrulline" /ligand_id="ChEBI:CHEBI:57743     E  

Additional information [?]

Size range 444-482 amino acids
Related rules MF_00005 (ASSY)
Fusion None
Comments Argininosuccinate synthase type 1 subfamily in MF_00005.