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HAMAP rule MF_00583

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General rule information [?]

Accession MF_00583
Dates 19-OCT-2002 (Created)
1-JUN-2023 (Last updated, Version 41)
Name RibP_PPkinase
Scope
Bacteria
Archaea
Templates P14193 (KPRS_BACSU); P0A717 (KPRS_ECOLI); Q97CA5 (KPRS_THEVO); Q58761 (KPRS_METJA); P9WKE3 (KPRS_MYCTU): [Recover all]
case <OC:Bacteria>
Triggered by
end case

case <OC:Archaea>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
KPRS
case <OC:Bacteria> and not <FTTag:Div>
Protein name
RecName: Full=Putative ribose-phosphate pyrophosphokinase;
Short=RPPK;
EC 2.7.6.1;
else
Protein name
RecName: Full=Ribose-phosphate pyrophosphokinase;
Short=RPPK;
EC 2.7.6.1;
end case
Protein name
AltName: Full=5-phospho-D-ribosyl alpha-1-diphosphate synthase;
AltName: Full=Phosphoribosyl diphosphate synthase;
AltName: Full=Phosphoribosyl pyrophosphate synthase;
Short=P-Rib-PP synthase;
Short=PRPP synthase;
Short=PRPPase;
Gene name
prs

Comments [?]

Function Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).
Catalytic activity RHEA:15609: ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)
EC 2.7.6.1
case <OC:Bacteria> and not <FTTag:Metal>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
else
Cofactor Mg(2+)
Note: Binds 2 Mg(2+) ions per subunit.
end case
Pathway Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.
case <OC:Bacteria>
Subunit Homohexamer.
end case
Subcellular location Cytoplasm.
case <OC:Archaea>
Similarity Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.
else
Similarity Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.
end case
case <OC:Bacteria> and not <FTTag:Div>
Caution Part of a set of proteins in which some residues (ACT_SITE, NP_BIND, REGION and BINDING) are not conserved.
end case

Keywords [?]

ATP-binding
Cytoplasm
Kinase
Magnesium
Metal-binding
Nucleotide-binding
Nucleotide biosynthesis
Transferase

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0004749; Molecular function: ribose phosphate diphosphokinase activity.
GO:0009156; Biological process: ribonucleoside monophosphate biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00156; Pribosyltran; 1;
NCBIfam TIGR01251; RibP_PPkin; 1;
PROSITE PS00114; PRPP_SYNTHASE; 1;

Features [?]

case <OC:Bacteria>
From: KPRS_BACSU (P14193)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     43     45       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [DN]-x-[EN]  
BINDING (Optional)     102     103       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     R-Q  
BINDING     228     232       /ligand="D-ribose 5-phosphate" /ligand_id="ChEBI:CHEBI:78346     [DN]-[TS]-[AG]-x-[TS]  
ACT_SITE     198     198           Div   K  
BINDING     136     136       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="#     H  
BINDING     175     175       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="#   Metal   D  
BINDING     200     200       /ligand="D-ribose 5-phosphate" /ligand_id="ChEBI:CHEBI:78346     R  
BINDING     224     224       /ligand="D-ribose 5-phosphate" /ligand_id="ChEBI:CHEBI:78346     D  
end case
case <OC:Archaea>
From: KPRS_THEVO (Q97CA5)
BINDING (Optional)     34     36       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     D-x-E  
BINDING (Optional)     91     92       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     R-Q  
BINDING     214     218       /ligand="D-ribose 5-phosphate" /ligand_id="ChEBI:CHEBI:78346     S-T-G-x-T  
ACT_SITE     184     184             K  
BINDING     124     124       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     H  
BINDING     161     161       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2     D  
BINDING     186     186       /ligand="D-ribose 5-phosphate" /ligand_id="ChEBI:CHEBI:78346     R  
BINDING     210     210       /ligand="D-ribose 5-phosphate" /ligand_id="ChEBI:CHEBI:78346     D  
end case

Additional information [?]

case <OC:Bacteria>
Size range 309-344 amino acids
end case
case <OC:Archaea>
Size range 271-300 amino acids
end case
Related rules None
Fusion None


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