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HAMAP rule MF_00583

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General rule information [?]

Accession MF_00583
Dates 19-OCT-2002 (Created)
19-JAN-2024 (Last updated, Version 42)
Name RibP_PPkinase
Scope(s) Bacteria
Archaea
Template(s) P14193 (KPRS_BACSU); P0A717 (KPRS_ECOLI); Q97CA5 (KPRS_THEVO); Q58761 (KPRS_METJA); P9WKE3 (KPRS_MYCTU); [ Recover all ]
Triggered by
case c? <OC:Bacteria>
HAMAP; MF_00583_B (Get profile general information and statistics)
end case
case c? <OC:Archaea>
HAMAP; MF_00583_A (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier KPRS
case <OC:Bacteria> and not <FTTag:Div>
Protein name RecName: Full=Putative ribose-phosphate pyrophosphokinase;
                 Short=RPPK;
                 EC=2.7.6.1;
else
Protein name RecName: Full=Ribose-phosphate pyrophosphokinase;
                 Short=RPPK;
                 EC=2.7.6.1;
end case
Protein name AltName: Full=5-phospho-D-ribosyl alpha-1-diphosphate synthase;
AltName: Full=Phosphoribosyl diphosphate synthase;
AltName: Full=Phosphoribosyl pyrophosphate synthase;
                 Short=P-Rib-PP synthase;
                 Short=PRPP synthase;
                 Short=PRPPase;
Gene name Name=prs;

Comments [?]

FUNCTIONInvolved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib- 5-P).
CATALYTIC ACTIVITY Reaction=ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1- diphosphate + AMP + H(+); Xref=Rhea:RHEA:15609, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:58017, ChEBI:CHEBI:78346, ChEBI:CHEBI:456215; EC=2.7.6.1;
case <OC:Bacteria> and not <FTTag:Metal>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
else
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 Mg(2+) ions per subunit.;
end case
PATHWAYMetabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.
case <OC:Bacteria>
SUBUNITHomohexamer.
end case
SUBCELLULAR LOCATIONCytoplasm.
case <OC:Archaea>
SIMILARITYBelongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.
else
SIMILARITYBelongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.
end case
case <OC:Bacteria> and not <FTTag:Div>
CAUTIONPart of a set of proteins in which some residues (ACT_SITE, NP_BIND, REGION and BINDING) are not conserved.
end case

Keywords [?]

ATP-binding
Cytoplasm
Kinase
Magnesium
Metal-binding
Nucleotide-binding
Nucleotide biosynthesis
Transferase

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0004749; Molecular function:ribose phosphate diphosphokinase activity
GO:0006015; Biological process:5-phosphoribose 1-diphosphate biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00156; Pribosyltran; 1;
NCBIfam TIGR01251; RibP_PPkin; 1;
PROSITE PS00114; PRPP_SYNTHASE; 1;

Features [?]

From: KPRS_BACSU (P14193)
Key From To Description Tag Condition FTGroup
case <OC:Bacteria>
BINDING 43 45 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 [DN]-x-[EN]
BINDING 102 103 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 R-Q
BINDING 228 232 /ligand="D-ribose 5-phosphate"
/ligand_id="ChEBI:CHEBI:78346"		 [DN]-[TS]-[AG]-x-[TS]
ACT_SITE 198 198 Div K
BINDING 136 136 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="#"		 H
BINDING 175 175 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="#"		 Metal D
BINDING 200 200 /ligand="D-ribose 5-phosphate"
/ligand_id="ChEBI:CHEBI:78346"		 R
BINDING 224 224 /ligand="D-ribose 5-phosphate"
/ligand_id="ChEBI:CHEBI:78346"		 D
end case
From: KPRS_THEVO (Q97CA5)
Key From To Description Tag Condition FTGroup
case <OC:Archaea>
BINDING 34 36 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 D-x-E
BINDING 91 92 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 R-Q
BINDING 214 218 /ligand="D-ribose 5-phosphate"
/ligand_id="ChEBI:CHEBI:78346"		 S-T-G-x-T
ACT_SITE 184 184 K
BINDING 124 124 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"		 H
BINDING 161 161 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"		 D
BINDING 186 186 /ligand="D-ribose 5-phosphate"
/ligand_id="ChEBI:CHEBI:78346"		 R
BINDING 210 210 /ligand="D-ribose 5-phosphate"
/ligand_id="ChEBI:CHEBI:78346"		 D
end case

Additional information [?]

Size range 271-300 amino acids
Related rules None
Fusion Nter: None Cter: None



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