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HAMAP rule MF_00614

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General rule information [?]

Accession MF_00614
Dates 31-JAN-2003 (Created)
1-JUN-2023 (Last updated, Version 23)
Name Fen
Scope(s) Archaea
Template(s) Q58839 (FEN_METJA); O93634 (FEN_PYRFU); [ Recover all ]
Triggered by
case c? <OC:Archaea>
HAMAP; MF_00614 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier FEN
Protein name RecName: Full=Flap endonuclease 1;
AltName: Full=Flap structure-specific endonuclease 1;
Gene name Name=fen;

Comments [?]

FUNCTIONStructure-specific nuclease with 5'-flap endonuclease and 5'- 3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.;
SUBUNITInteracts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.
SIMILARITYBelongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003677; Molecular function:DNA binding
GO:0008409; Molecular function:5'-3' exonuclease activity
GO:0017108; Molecular function:5'-flap endonuclease activity
GO:0000287; Molecular function:magnesium ion binding
GO:0043137; Biological process:DNA replication, removal of RNA primer
GO:0006281; Biological process:DNA repair

Cross-references [?]

Pfam PF00867; XPG_I; 1;
Pfam PF00752; XPG_N; 1;
NCBIfam TIGR03674; Fen_arch; 1;
PROSITE PS00841; XPG_1; 1;

Features [?]

From: FEN_METJA (Q58839)
Key From To Description Tag Condition FTGroup
REGION 1 98 /note="N-domain"
REGION 116 245 /note="I-domain"
REGION 317 325 /note="Interaction with PCNA" x-Q-x(2)-[ILM]-x(2)-[FYW]-[FYILAT]
BINDING 27 27 /ligand="Mg(2+)"
BINDING 80 80 /ligand="Mg(2+)"
BINDING 152 152 /ligand="Mg(2+)"
BINDING 154 154 /ligand="Mg(2+)"
BINDING 173 173 /ligand="Mg(2+)"
BINDING 175 175 /ligand="Mg(2+)"
BINDING 224 224 /ligand="Mg(2+)"

Additional information [?]

Size range 326-351 amino acids
Related rules None
Fusion Nter: None Cter: None

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