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HAMAP rule MF_00614

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General rule information [?]

Accession MF_00614
Dates 31-JAN-2003 (Created)
1-JUN-2023 (Last updated, Version 23)
Name Fen
Templates Q58839 (FEN_METJA); O93634 (FEN_PYRFU): [Recover all]
case <OC:Archaea>
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Flap endonuclease 1;
EC 3.1.-.-;
AltName: Full=Flap structure-specific endonuclease 1;
Gene name

Comments [?]

Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.
Cofactor Mg(2+)
Note: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.
Subunit Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.
Similarity Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0008409; Molecular function: 5'-3' exonuclease activity.
GO:0017108; Molecular function: 5'-flap endonuclease activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0043137; Biological process: DNA replication, removal of RNA primer.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF00867; XPG_I; 1;
PF00752; XPG_N; 1;
NCBIfam TIGR03674; Fen_arch; 1;
PROSITE PS00841; XPG_1; 1;

Features [?]

From: FEN_METJA (Q58839)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     1     98       N-domain        
REGION     116     245       I-domain        
REGION (Optional)     317     325       Interaction with PCNA     x-Q-x(2)-[ILM]-x(2)-[FYW]-[FYILAT]  
BINDING     27     27       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     D  
BINDING     80     80       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     D  
BINDING     152     152       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     E  
BINDING     154     154       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     E  
BINDING     173     173       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2     D  
BINDING     175     175       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2     D  
BINDING     224     224       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2     D  

Additional information [?]

Size range 326-351 amino acids
Related rules None
Fusion None