HAMAP rule MF_00614

General rule information [?]

Accession	MF_00614
Dates	31-JAN-2003 (Created) 1-JUN-2023 (Last updated, Version 23)

Name

Fen

Scope

Archaea

Templates

Q58839 (FEN_METJA); O93634 (FEN_PYRFU): [Recover all]

case <OC:Archaea>

Triggered by

HAMAP; MF_00614 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

FEN

Protein name

RecName:		Full=Flap endonuclease 1;
		Short=FEN-1;
		EC 3.1.-.-;
AltName:		Full=Flap structure-specific endonuclease 1;

Gene name

fen

Comments [?]

Function	Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.
Cofactor	Mg(2+) Note: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.
Subunit	Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.
Similarity	Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Keywords [?]

DNA damage
DNA repair
DNA replication
Endonuclease
Exonuclease
Hydrolase
Magnesium
Metal-binding
Nuclease

Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0008409; Molecular function: 5'-3' exonuclease activity.
GO:0017108; Molecular function: 5'-flap endonuclease activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0043137; Biological process: DNA replication, removal of RNA primer.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam	PF00867; XPG_I; 1;
	PF00752; XPG_N; 1;
PRINTS	PR00853; XPGRADSUPER; 1;
NCBIfam	TIGR03674; Fen_arch; 1;
PROSITE	PS00841; XPG_1; 1;

Features [?]

From: FEN_METJA (Q58839)

Key From To Description Tag Condition FTGroup

REGION 1 98 N-domain

REGION 116 245 I-domain

REGION (Optional) 317 325 Interaction with PCNA x-Q-x(2)-[ILM]-x(2)-[FYW]-[FYILAT]

BINDING 27 27 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 D

BINDING 80 80 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 D

BINDING 152 152 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 E

BINDING 154 154 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 E

BINDING 173 173 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 D

BINDING 175 175 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 D

BINDING 224 224 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 D

Additional information [?]

Size range	326-351 amino acids
Related rules	None
Fusion	None

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