Due to maintenance work, this service will be unavailable from
Mon Nov 11 17:30 until
Tue Nov 12 09:00
CET.
Apologies for the inconvenience.
HAMAP rule MF_00614
General rule information
[?]
| Accession | MF_00614 |
| Dates | 31-JAN-2003 (Created)
1-JUN-2023 (Last updated, Version 23) |
| Name | Fen |
| Scope(s) |
Archaea |
| Template(s) | Q58839 (FEN_METJA); O93634 (FEN_PYRFU); [ Recover all ] |
| Triggered by |
case c? <OC:Archaea>
HAMAP; MF_00614 (Get profile general information and statistics) end case
|
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | FEN |
| Protein name | RecName: Full=Flap endonuclease 1; Short=FEN-1; EC=3.1.-.-; AltName: Full=Flap structure-specific endonuclease 1; |
| Gene name | Name=fen; |
Comments
[?]
| FUNCTION | Structure-specific nuclease with 5'-flap endonuclease and 5'- 3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA. |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.; |
| SUBUNIT | Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity. |
| SIMILARITY | Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily. |
Keywords
[?]
| DNA damage |
| DNA repair |
| DNA replication |
| Endonuclease |
| Exonuclease |
| Hydrolase |
| Magnesium |
| Metal-binding |
| Nuclease |
Gene Ontology
[?]
| GO:0003677; Molecular function:DNA binding |
| GO:0008409; Molecular function:5'-3' exonuclease activity |
| GO:0017108; Molecular function:5'-flap endonuclease activity |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0043137; Biological process:DNA replication, removal of RNA primer |
| GO:0006281; Biological process:DNA repair |
Cross-references
[?]
| Pfam | PF00867; XPG_I; 1; |
| Pfam | PF00752; XPG_N; 1; |
| PRINTS | PR00853; XPGRADSUPER; 1; |
| NCBIfam | TIGR03674; Fen_arch; 1; |
| PROSITE | PS00841; XPG_1; 1; |
Features
[?]
| From: FEN_METJA (Q58839) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 1 | 98 | /note="N-domain" | |||||||||
| REGION | 116 | 245 | /note="I-domain" | |||||||||
| REGION | 317 | 325 | /note="Interaction with PCNA" | x-Q-x(2)-[ILM]-x(2)-[FYW]-[FYILAT] | ||||||||
| BINDING | 27 | 27 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
| BINDING | 80 | 80 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
| BINDING | 152 | 152 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
| BINDING | 154 | 154 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
| BINDING | 173 | 173 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
| BINDING | 175 | 175 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
| BINDING | 224 | 224 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
Additional information
[?]
| Size range | 326-351 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |