HAMAP rule MF_00639

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00639
Accession	MF_00639
Dates	28-FEB-2005 (Created) 02-SEP-2024 (Last updated, Version 31)
Name	MurD
Scope(s)	Bacteria
Template(s)	P14900; Q9HVZ9; [ Recover all ]
Triggered by	HAMAP; MF_00639 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	MURD
Protein name	RecName: Full=UDP-N-acetylmuramoylalanine--D-glutamate ligase; EC=6.3.2.9; AltName: Full=D-glutamic acid-adding enzyme; AltName: Full=UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase;
Gene name	Name=murD;

Comments [?]

FUNCTION	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).
CATALYTIC ACTIVITY	Reaction=UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate + ATP = UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + ADP + phosphate + H(+); Xref=Rhea:RHEA:16429, ChEBI:CHEBI:15378, ChEBI:CHEBI:29986, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:83898, ChEBI:CHEBI:83900, ChEBI:CHEBI:456216; EC=6.3.2.9;
PATHWAY	Cell wall biogenesis; peptidoglycan biosynthesis.
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the MurCDEF family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding

GO:0008764; Molecular function:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity

GO:0009252; Biological process:peptidoglycan biosynthetic process

GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Features [?]

From: MURD_ECOLI (P14900)

Key

From

Description

Tag

Condition

FTGroup

BINDING

112

118

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

G-[STA]-x-[GA]-K-[GST]-[ST]

Additional information [?]

Size range	380-660 amino acids
Related rules	MF_02208
Fusion	Nter: None Cter: None
Comments	Also matches MurD2 subfamily (MF_02208). Insertion in POLSJ (not shown in alignment and not used in size range)