HAMAP rule MF_00641
General rule information
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Accession | MF_00641 |
Dates | 26-APR-2003 (Created)
1-JUN-2023 (Last updated, Version 30) |
Name | Malate_synth_G |
Scope(s) |
Bacteria |
Template(s) | P42450 (MASZ_CORGL); P37330 (MASZ_ECOLI); P9WK17 (MASZ_MYCTU); [ Recover all ] |
Triggered by |
HAMAP; MF_00641 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | MASZ |
Protein name | RecName: Full=Malate synthase G; EC=2.3.3.9; |
Gene name | Name=glcB; |
Comments
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FUNCTION | Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl- CoA) and glyoxylate to form malate and CoA. |
CATALYTIC ACTIVITY | Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+); Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.9; |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
PATHWAY | Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. |
SUBUNIT | Monomer. |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the malate synthase family. GlcB subfamily. |
Keywords
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Gene Ontology
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GO:0004474; Molecular function:malate synthase activity |
GO:0006097; Biological process:glyoxylate cycle |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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From: MASZ_ECOLI (P37330) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 125 | 126 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
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BINDING | 452 | 455 | /ligand="glyoxylate" /ligand_id="ChEBI:CHEBI:36655" |
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ACT_SITE | 338 | 338 | /note="Proton acceptor" | R | ||||||||
ACT_SITE | 631 | 631 | /note="Proton donor" | D | ||||||||
BINDING | 427 | 427 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
BINDING | 455 | 455 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
BINDING | 118 | 118 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
V | ||||||||
BINDING | 274 | 274 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
S | ||||||||
BINDING | 311 | 311 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
R | ||||||||
BINDING | 338 | 338 | /ligand="glyoxylate" /ligand_id="ChEBI:CHEBI:36655" |
R | ||||||||
BINDING | 427 | 427 | /ligand="glyoxylate" /ligand_id="ChEBI:CHEBI:36655" |
E | ||||||||
BINDING | 536 | 536 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
P | ||||||||
MOD_RES | 617 | 617 | /note="Cysteine sulfenic acid (-SOH)" | C | ||||||||
MOD_RES | 688 | 688 | /note="Cysteine sulfenic acid (-SOH)" | C |
Additional information
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Size range | 721-748 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |