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HAMAP rule MF_00641

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General rule information [?]

Accession MF_00641
Dates 26-APR-2003 (Created)
1-JUN-2023 (Last updated, Version 30)
Name Malate_synth_G
Scope(s) Bacteria
Template(s) P42450 (MASZ_CORGL); P37330 (MASZ_ECOLI); P9WK17 (MASZ_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_00641 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MASZ
Protein name RecName: Full=Malate synthase G;
Gene name Name=glcB;

Comments [?]

FUNCTIONInvolved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl- CoA) and glyoxylate to form malate and CoA.
CATALYTIC ACTIVITY Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+); Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYCarbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
SIMILARITYBelongs to the malate synthase family. GlcB subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004474; Molecular function:malate synthase activity
GO:0006097; Biological process:glyoxylate cycle
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01274; Malate_synthase; 1;
NCBIfam TIGR01345; Malate_syn_G; 1;

Features [?]

From: MASZ_ECOLI (P37330)
Key From To Description Tag Condition FTGroup
BINDING 125 126 /ligand="acetyl-CoA"
BINDING 452 455 /ligand="glyoxylate"
ACT_SITE 338 338 /note="Proton acceptor" R
ACT_SITE 631 631 /note="Proton donor" D
BINDING 427 427 /ligand="Mg(2+)"
BINDING 455 455 /ligand="Mg(2+)"
BINDING 118 118 /ligand="acetyl-CoA"
BINDING 274 274 /ligand="acetyl-CoA"
BINDING 311 311 /ligand="acetyl-CoA"
BINDING 338 338 /ligand="glyoxylate"
BINDING 427 427 /ligand="glyoxylate"
BINDING 536 536 /ligand="acetyl-CoA"
MOD_RES 617 617 /note="Cysteine sulfenic acid (-SOH)" C
MOD_RES 688 688 /note="Cysteine sulfenic acid (-SOH)" C

Additional information [?]

Size range 721-748 amino acids
Related rules None
Fusion Nter: None Cter: None

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