HAMAP rule MF_00641
General rule information
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| Accession | MF_00641 |
| Dates | 26-APR-2003 (Created)
1-JUN-2023 (Last updated, Version 30) |
| Name | Malate_synth_G |
| Scope(s) |
Bacteria |
| Template(s) | P42450 (MASZ_CORGL); P37330 (MASZ_ECOLI); P9WK17 (MASZ_MYCTU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00641 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MASZ |
| Protein name | RecName: Full=Malate synthase G; EC=2.3.3.9; |
| Gene name | Name=glcB; |
Comments
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| FUNCTION | Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl- CoA) and glyoxylate to form malate and CoA. |
| CATALYTIC ACTIVITY | Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+); Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.9; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| PATHWAY | Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the malate synthase family. GlcB subfamily. |
Keywords
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Gene Ontology
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| GO:0004474; Molecular function:malate synthase activity |
| GO:0006097; Biological process:glyoxylate cycle |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: MASZ_ECOLI (P37330) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 125 | 126 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
|||||||||
| BINDING | 452 | 455 | /ligand="glyoxylate" /ligand_id="ChEBI:CHEBI:36655" |
|||||||||
| ACT_SITE | 338 | 338 | /note="Proton acceptor" | R | ||||||||
| ACT_SITE | 631 | 631 | /note="Proton donor" | D | ||||||||
| BINDING | 427 | 427 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
| BINDING | 455 | 455 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 118 | 118 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
V | ||||||||
| BINDING | 274 | 274 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
S | ||||||||
| BINDING | 311 | 311 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
R | ||||||||
| BINDING | 338 | 338 | /ligand="glyoxylate" /ligand_id="ChEBI:CHEBI:36655" |
R | ||||||||
| BINDING | 427 | 427 | /ligand="glyoxylate" /ligand_id="ChEBI:CHEBI:36655" |
E | ||||||||
| BINDING | 536 | 536 | /ligand="acetyl-CoA" /ligand_id="ChEBI:CHEBI:57288" |
P | ||||||||
| MOD_RES | 617 | 617 | /note="Cysteine sulfenic acid (-SOH)" | C | ||||||||
| MOD_RES | 688 | 688 | /note="Cysteine sulfenic acid (-SOH)" | C | ||||||||
Additional information
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| Size range | 721-748 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |