HAMAP rule MF_00658
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00658 |
| Accession | MF_00658 |
| Dates | 17-JUN-2003 (Created)
8-OCT-2024 (Last updated, Version 37) |
| Name | 23SrRNA_methyltr_H |
| Scope(s) |
Bacteria Archaea Methanobacteriati |
| Template(s) | P0A8I8 (RLMH_ECOLI); P0C1V0 (RLMH_STAAU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00658 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RLMH |
| case <OC:Bacteria> | |
| Protein name | RecName: Full=Ribosomal RNA large subunit methyltransferase H; EC=2.1.1.177; AltName: Full=23S rRNA (pseudouridine1915-N3)-methyltransferase; AltName: Full=23S rRNA m3Psi1915 methyltransferase; AltName: Full=rRNA (pseudouridine-N3-)-methyltransferase RlmH; |
| else case <OC:Archaea> | |
| Protein name | RecName: Full=Putative ribosomal RNA large subunit methyltransferase H; EC=2.1.1.177; AltName: Full=23S rRNA (pseudouridine1915-N3)-methyltransferase; AltName: Full=rRNA (pseudouridine-N3-)-methyltransferase RlmH; |
| end case | |
| Gene name | Name=rlmH; |
Comments
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| FUNCTION | Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA. |
| CATALYTIC ACTIVITY | Reaction=pseudouridine(1915) in 23S rRNA + S-adenosyl-L-methionine = N(3)-methylpseudouridine(1915) in 23S rRNA + S-adenosyl-L- homocysteine + H(+); Xref=Rhea:RHEA:42752, Rhea:RHEA-COMP:10221, Rhea:RHEA- COMP:10222, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65314, ChEBI:CHEBI:74486; EC=2.1.1.177; |
| case <OC:Bacteria> | |
| SUBUNIT | Homodimer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the RNA methyltransferase RlmH family. |
Keywords
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Gene Ontology
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| GO:0070038; Molecular function:rRNA (pseudouridine-N3-)-methyltransferase activity |
| GO:0031167; Biological process:rRNA methylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF02590; SPOUT_MTase; 1; |
| PIRSF | PIRSF004505; MT_bac; 1; |
| NCBIfam | TIGR00246; tRNA_RlmH_YbeA; 1; |
Features
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| From: RLMH_ECOLI (P0A8I8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 122 | 127 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[LIVFM]-[GAST]-x-[LIM]-x-[LMFYW] | ||||||||
| BINDING | 72 | 72 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[LI] | ||||||||
| BINDING | 103 | 103 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[GA] | ||||||||
Additional information
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| Size range | 120-210 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |