HAMAP rule MF_00720

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00720
Accession	MF_00720
Dates	28-FEB-2005 (Created) 04-FEB-2025 (Last updated, Version 16)
Name	PriB
Scope(s)	Bacteria Betaproteobacteria Gammaproteobacteria
Template(s)	P07013; Q5F924; A6THB2; [ Recover all ]
Triggered by	HAMAP; MF_00720 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	PRIB
Protein name	RecName: Full=Replication restart protein PriB;
Gene name	Name=priB;

Comments [?]

FUNCTION	Involved in the restart of stalled replication forks, which reloads the replicative helicase on sites other than the origin of replication; the PriA-PriB pathway is the major replication restart pathway. During primosome assembly it facilitates complex formation between PriA and DnaT on DNA; stabilizes PriA on DNA. Stimulates the DNA unwinding activity of PriA helicase.
SUBUNIT	Homodimer. Interacts with PriA and DnaT. Component of the replication restart primosome. Primosome assembly occurs via a 'hand- off' mechanism. PriA binds to replication forks, subsequently PriB then DnaT bind; DnaT then displaces ssDNA to generate the helicase loading substrate.
SIMILARITY	Belongs to the PriB family.

Keywords [?]

Gene Ontology [?]

GO:0003697; Molecular function:single-stranded DNA binding

GO:0006261; Biological process:DNA-templated DNA replication

GO:1990077; Cellular component:primosome complex

Cross-references [?]

Features [?]

Additional information [?]

Size range	90-130 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	For a reference on the evolution of this family, see: MEDLINE=22749900; PubMed=12867746; Ponomarev V.A., Makarova K.S., Aravind L., Koonin E.V.; "Gene duplication with displacement and rearrangement: origin of the bacterial replication protein PriB from the single-stranded DNA-binding protein Ssb."; J. Mol. Microbiol. Biotechnol. 5:225-229(2003).