HAMAP rule MF_00723
General rule information
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Accession | MF_00723 |
Dates | 1-NOV-2003 (Created)
1-JUN-2023 (Last updated, Version 31) |
Name | Formald_GSH |
Scope(s) |
Bacteria Pseudomonadota |
Template(s) | Q51669 (GFA_PARDE); [ Recover all ] |
Triggered by |
case c? <OC:Bacteria>
HAMAP; MF_00723 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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Identifier | GFA |
Protein name | RecName: Full=Glutathione-dependent formaldehyde-activating enzyme; EC=4.4.1.22; AltName: Full=S-(hydroxymethyl)glutathione synthase; |
Gene name | Name=gfa; |
Comments
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FUNCTION | Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione. |
CATALYTIC ACTIVITY | Reaction=S-(hydroxymethyl)glutathione = formaldehyde + glutathione; Xref=Rhea:RHEA:22488, ChEBI:CHEBI:16842, ChEBI:CHEBI:57925, ChEBI:CHEBI:58758; EC=4.4.1.22; |
case <FTGroup:1> | |
COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per subunit.; |
end case | |
PATHWAY | One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (glutathione route): step 1/3. |
SIMILARITY | Belongs to the Gfa family. |
Keywords
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Lyase | |
case <FTGroup:1> | |
Metal-binding | |
Zinc | |
end case |
Gene Ontology
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GO:0051907; Molecular function:S-(hydroxymethyl)glutathione synthase activity | |
GO:0046294; Biological process:formaldehyde catabolic process | |
case <FTGroup:1> | |
GO:0008270; Molecular function:zinc ion binding | |
end case |
Cross-references
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Pfam | PF04828; GFA; 1; |
PIRSF | PIRSF033318; Formald_GSH; 1; |
NCBIfam | TIGR02820; Formald_GSH; 1; |
PROSITE | PS51891; CENP_V_GFA; 1; |
Features
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From: GFA_PARDE (Q51669) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 31 | 31 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="structural" |
C | 1 | |||||||
BINDING | 33 | 33 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="structural" |
C | 1 | |||||||
BINDING | 52 | 52 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" /ligand_note="catalytic" |
C | 1 | |||||||
BINDING | 54 | 54 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" /ligand_note="catalytic" |
C | 1 | |||||||
BINDING | 57 | 57 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" /ligand_note="catalytic" |
C | 1 | |||||||
BINDING | 99 | 99 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="structural" |
C | 1 | |||||||
BINDING | 102 | 102 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="structural" |
C | 1 |
Additional information
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Size range | 187-194 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |