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HAMAP rule MF_00723

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General rule information [?]

Accession MF_00723
Dates 1-NOV-2003 (Created)
1-JUN-2023 (Last updated, Version 31)
Name Formald_GSH
Scope(s) Bacteria
Pseudomonadota
Template(s) Q51669 (GFA_PARDE); [ Recover all ]
Triggered by
case c? <OC:Bacteria>
HAMAP; MF_00723 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GFA
Protein name RecName: Full=Glutathione-dependent formaldehyde-activating enzyme;
                 EC=4.4.1.22;
AltName: Full=S-(hydroxymethyl)glutathione synthase;
Gene name Name=gfa;

Comments [?]

FUNCTIONCatalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione.
CATALYTIC ACTIVITY Reaction=S-(hydroxymethyl)glutathione = formaldehyde + glutathione; Xref=Rhea:RHEA:22488, ChEBI:CHEBI:16842, ChEBI:CHEBI:57925, ChEBI:CHEBI:58758; EC=4.4.1.22;
case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per subunit.;
end case
PATHWAYOne-carbon metabolism; formaldehyde degradation; formate from formaldehyde (glutathione route): step 1/3.
SIMILARITYBelongs to the Gfa family.

Keywords [?]

Lyase
case <FTGroup:1>
Metal-binding
Zinc
end case

Gene Ontology [?]

GO:0051907; Molecular function:S-(hydroxymethyl)glutathione synthase activity
GO:0046294; Biological process:formaldehyde catabolic process
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case

Cross-references [?]

Pfam PF04828; GFA; 1;
PIRSF PIRSF033318; Formald_GSH; 1;
NCBIfam TIGR02820; Formald_GSH; 1;
PROSITE PS51891; CENP_V_GFA; 1;

Features [?]

From: GFA_PARDE (Q51669)
Key From To Description Tag Condition FTGroup
BINDING 31 31 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
/ligand_note="structural"		 C 1
BINDING 33 33 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
/ligand_note="structural"		 C 1
BINDING 52 52 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
/ligand_note="catalytic"		 C 1
BINDING 54 54 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
/ligand_note="catalytic"		 C 1
BINDING 57 57 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"
/ligand_note="catalytic"		 C 1
BINDING 99 99 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
/ligand_note="structural"		 C 1
BINDING 102 102 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"
/ligand_note="structural"		 C 1

Additional information [?]

Size range 187-194 amino acids
Related rules None
Fusion Nter: None Cter: None



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