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Annotation rule MF_00743
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General rule information [?]

Accession MF_00743
Dates 4-DEC-2003 (Created)
18-NOV-2019 (Last updated, Version 28)
Name FumaraseC
Scope
Bacteria
Archaea
Templates P05042 (FUMC_ECOLI); P9WN93 (FUMC_MYCTU); P39461 (FUMC_SACS2): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
FUMC
Protein name
RecName: Full=Fumarate hydratase class II;
Short=Fumarase C;
EC 4.2.1.2;
AltName: Full=Aerobic fumarase;
AltName: Full=Iron-independent fumarase;
Gene name
fumC

Comments [?]

Function Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.
Catalytic activity RHEA:12460: (S)-malate = fumarate + H2O
EC 4.2.1.2
Pathway Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Subunit Homotetramer.
Subcellular location Cytoplasm.
Miscellaneous There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.
Similarity Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004333; Molecular function: fumarate hydratase activity.
GO:0006099; Biological process: tricarboxylic acid cycle.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF10415; FumaraseC_C; 1;
PF00206; Lyase_1; 1;
PRINTS PR00149; FUMRATELYASE; 1;
TIGRFAMs TIGR00979; FumC_II; 1;
PROSITE PS00163; FUMARATE_LYASES; 1;

Features [?]

From: FUMC_MYCTU (P9WN93)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     104     106       Substrate binding     S-x-T  
REGION     128     131       Substrate binding (B site)     [HN]-x-N-D  
REGION     138     140       Substrate binding     S-[ST]-N  
REGION     324     326       Substrate binding     K-x-N  
ACT_SITE     187     187       Proton donor/acceptor     H  
ACT_SITE     318     318             S  
BINDING     186     186       Substrate     T  
BINDING     319     319       Substrate     S  
SITE     331     331       Important for catalytic activity     E  

Additional information [?]

Size range 438-484 amino acids
Related rules None
Fusion None