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HAMAP rule MF_00743
General rule information
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| Accession | MF_00743 |
| Dates | 4-DEC-2003 (Created)
28-NOV-2023 (Last updated, Version 32) |
| Name | FumaraseC |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P05042 (FUMC_ECOLI); P9WN93 (FUMC_MYCTU); P39461 (FUMC_SACS2); [ Recover all ] |
| Triggered by |
HAMAP; MF_00743 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | FUMC |
| Protein name | RecName: Full=Fumarate hydratase class II; Short=Fumarase C; EC=4.2.1.2; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase; |
| Gene name | Name=fumC; |
Comments
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| FUNCTION | Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate. |
| CATALYTIC ACTIVITY | Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460, ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2; |
| PATHWAY | Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. |
| SUBUNIT | Homotetramer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| MISCELLANEOUS | There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors. |
| SIMILARITY | Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. |
Keywords
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Gene Ontology
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| GO:0004333; Molecular function:fumarate hydratase activity |
| GO:0006099; Biological process:tricarboxylic acid cycle |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF10415; FumaraseC_C; 1; |
| Pfam | PF00206; Lyase_1; 1; |
| PRINTS | PR00149; FUMRATELYASE; 1; |
| NCBIfam | TIGR00979; FumC_II; 1; |
| PROSITE | PS00163; FUMARATE_LYASES; 1; |
Features
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| From: FUMC_MYCTU (P9WN93) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 104 | 106 | /ligand="substrate" | S-x-T | ||||||||
| BINDING | 128 | 131 | /ligand="substrate" /note="in site B" |
[HN]-x-N-D | ||||||||
| BINDING | 138 | 140 | /ligand="substrate" | S-[ST]-N | ||||||||
| BINDING | 324 | 326 | /ligand="substrate" | K-x-N | ||||||||
| ACT_SITE | 187 | 187 | /note="Proton donor/acceptor" | H | ||||||||
| ACT_SITE | 318 | 318 | S | |||||||||
| BINDING | 186 | 186 | /ligand="substrate" | T | ||||||||
| BINDING | 319 | 319 | /ligand="substrate" | S | ||||||||
| SITE | 331 | 331 | /note="Important for catalytic activity" | E | ||||||||
Additional information
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| Size range | 438-484 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |