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| Annotation rule MF_00743 |
General rule information
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| Accession | MF_00743 |
| Dates | 4-DEC-2003 (Created) 18-NOV-2019 (Last updated, Version 28) |
| Name | FumaraseC |
| Scope | Bacteria
Archaea |
| Templates | P05042 (FUMC_ECOLI); P9WN93 (FUMC_MYCTU); P39461 (FUMC_SACS2): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate. |
| Catalytic activity | RHEA:12460: (S)-malate = fumarate + H2O
EC 4.2.1.2 |
| Pathway | Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. |
| Subunit | Homotetramer. |
| Subcellular location | Cytoplasm. |
| Miscellaneous | There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors. |
| Similarity | Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. |
Keywords
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Gene Ontology
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GO:0004333; Molecular function: fumarate hydratase activity.
GO:0006099; Biological process: tricarboxylic acid cycle.
GO:0005737; Cellular component: cytoplasm.
GO:0006099; Biological process: tricarboxylic acid cycle.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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| Pfam | PF10415; FumaraseC_C; 1; |
| PF00206; Lyase_1; 1; | |
| PRINTS | PR00149; FUMRATELYASE; 1; |
| TIGRFAMs | TIGR00979; FumC_II; 1; |
| PROSITE | PS00163; FUMARATE_LYASES; 1; |
Features
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| From: FUMC_MYCTU (P9WN93) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 104 | 106 | Substrate binding | S-x-T | ||||||||
| REGION | 128 | 131 | Substrate binding (B site) | [HN]-x-N-D | ||||||||
| REGION | 138 | 140 | Substrate binding | S-[ST]-N | ||||||||
| REGION | 324 | 326 | Substrate binding | K-x-N | ||||||||
| ACT_SITE | 187 | 187 | Proton donor/acceptor | H | ||||||||
| ACT_SITE | 318 | 318 | S | |||||||||
| BINDING | 186 | 186 | Substrate | T | ||||||||
| BINDING | 319 | 319 | Substrate | S | ||||||||
| SITE | 331 | 331 | Important for catalytic activity | E | ||||||||
Additional information
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| Size range | 438-484 amino acids |
| Related rules | None |
| Fusion | None |