![]() |
|
Annotation rule MF_00743 |
Accession | MF_00743 |
Dates | 4-DEC-2003 (Created) 18-NOV-2019 (Last updated, Version 28) |
Name | FumaraseC |
Scope | Bacteria
Archaea |
Templates | P05042 (FUMC_ECOLI); P9WN93 (FUMC_MYCTU); P39461 (FUMC_SACS2): [Recover all] |
Triggered by |
Identifier |
|
Protein name |
|
Gene name |
|
Function | Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate. |
Catalytic activity | RHEA:12460: (S)-malate = fumarate + H2O
EC 4.2.1.2 |
Pathway | Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. |
Subunit | Homotetramer. |
Subcellular location | Cytoplasm. |
Miscellaneous | There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors. |
Similarity | Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. |
Pfam | PF10415; FumaraseC_C; 1; |
PF00206; Lyase_1; 1; | |
PRINTS | PR00149; FUMRATELYASE; 1; |
TIGRFAMs | TIGR00979; FumC_II; 1; |
PROSITE | PS00163; FUMARATE_LYASES; 1; |
From: FUMC_MYCTU (P9WN93) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 104 | 106 | Substrate binding | S-x-T | ||||||||
REGION | 128 | 131 | Substrate binding (B site) | [HN]-x-N-D | ||||||||
REGION | 138 | 140 | Substrate binding | S-[ST]-N | ||||||||
REGION | 324 | 326 | Substrate binding | K-x-N | ||||||||
ACT_SITE | 187 | 187 | Proton donor/acceptor | H | ||||||||
ACT_SITE | 318 | 318 | S | |||||||||
BINDING | 186 | 186 | Substrate | T | ||||||||
BINDING | 319 | 319 | Substrate | S | ||||||||
SITE | 331 | 331 | Important for catalytic activity | E |
Size range | 438-484 amino acids |
Related rules | None |
Fusion | None |