HAMAP rule MF_00747
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00747 |
| Accession | MF_00747 |
| Dates | 6-JAN-2004 (Created)
2-SEP-2024 (Last updated, Version 35) |
| Name | AceK |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P11071 (ACEK_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00747 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ACEK |
| Protein name | RecName: Full=Isocitrate dehydrogenase kinase/phosphatase; Short=IDH kinase/phosphatase; Short=IDHK/P; EC=2.7.11.5; EC=3.1.3.-; |
| Gene name | Name=aceK; |
Comments
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| FUNCTION | Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation. |
| CATALYTIC ACTIVITY | Reaction=L-seryl-[isocitrate dehydrogenase] + ATP = O-phospho-L-seryl- [isocitrate dehydrogenase] + ADP + H(+); Xref=Rhea:RHEA:43540, Rhea:RHEA-COMP:10605, Rhea:RHEA-COMP:10606, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.5; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the AceK family. |
Keywords
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| Cytoplasm |
| ATP-binding |
| Glyoxylate bypass |
| Hydrolase |
| Kinase |
| Nucleotide-binding |
| Protein phosphatase |
| Serine/threonine-protein kinase |
| Transferase |
| Tricarboxylic acid cycle |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0016788; Molecular function:hydrolase activity, acting on ester bonds |
| GO:0008772; Molecular function:[isocitrate dehydrogenase (NADP+)] kinase activity |
| GO:0006097; Biological process:glyoxylate cycle |
| GO:0006099; Biological process:tricarboxylic acid cycle |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF06315; AceK; 1; |
Features
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| From: ACEK_ECOLI (P11071) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 315 | 321 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
A-x-G-x-x-G-[LIVMT] | ||||||||
| ACT_SITE | 371 | 371 | D | |||||||||
| BINDING | 336 | 336 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
Additional information
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| Size range | 564-625 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |