HAMAP rule MF_00782
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00782 |
| Accession | MF_00782 |
| Dates | 28-FEB-2005 (Created)
02-SEP-2024 (Last updated, Version 26) |
| Name | Glut_biosynth |
| Scope(s) |
Bacteria Bacillota Pseudomonadota |
| Template(s) | Q8DXM9; [ Recover all ] |
| Triggered by |
HAMAP; MF_00782 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GSHAB |
| Protein name | RecName: Full=Glutathione biosynthesis bifunctional protein GshAB; AltName: Full=Gamma-GCS-GS; Short=GCS-GS; Includes: RecName: Full=Glutamate--cysteine ligase; EC=6.3.2.2; AltName: Full=Gamma-ECS; Short=GCS; AltName: Full=Gamma-glutamylcysteine synthetase; Includes: RecName: Full=Glutathione synthetase; EC=6.3.2.3; AltName: Full=GSH synthetase; Short=GS; Short=GSH-S; Short=GSHase; AltName: Full=Glutathione synthase; |
| Gene name | Name=gshAB; Synonyms=gshF; |
Comments
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| FUNCTION | Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine. |
| CATALYTIC ACTIVITY | Reaction=L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+); Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235, ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2; |
| CATALYTIC ACTIVITY | Reaction=gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+); Xref=Rhea:RHEA:13557, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57305, ChEBI:CHEBI:57925, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.3; |
| PATHWAY | Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. |
| PATHWAY | Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. |
| SUBUNIT | Monomer. |
| SIMILARITY | In the N-terminal section; belongs to the glutamate-- cysteine ligase type 1 family. Type 2 subfamily. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004363; Molecular function:glutathione synthase activity |
| GO:0004357; Molecular function:glutamate-cysteine ligase activity |
| GO:0006750; Biological process:glutathione biosynthetic process |
Cross-references
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| Pfam | PF04262; Glu_cys_ligase; 1; |
| NCBIfam | TIGR01435; Glu_cys_lig_rel; 1; |
| PROSITE | PS50975; ATP_GRASP; 1; |
Features
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| From: GSHAB_STRA5 (Q8DXM9) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 333 | /note="Glutamate--cysteine ligase" | |||||||||
Additional information
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| Size range | 750-779 amino acids |
| Related rules |
MF_00578 |
| Fusion | Nter: None Cter: None |