HAMAP rule MF_00801
General rule information
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| Accession | MF_00801 |
| Dates | 24-NOV-2004 (Created)
19-NOV-2022 (Last updated, Version 29) |
| Name | Endonuclease_5 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P68739 (NFI_ECOLI); O30108 (NFI_ARCFU); Q9X2H9 (NFI_THEMA); [ Recover all ] |
| Triggered by |
HAMAP; MF_00801 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NFI |
| Protein name | RecName: Full=Endonuclease V; EC=3.1.21.7; AltName: Full=Deoxyinosine 3'endonuclease; AltName: Full=Deoxyribonuclease V; Short=DNase V; |
| Gene name | Name=nfi; |
Comments
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| FUNCTION | DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. |
| CATALYTIC ACTIVITY | Reaction=Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.; EC=3.1.21.7; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the endonuclease V family. |
Keywords
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| Cytoplasm | |
| DNA damage | |
| DNA repair | |
| Endonuclease | |
| Hydrolase | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
| Nuclease | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0043737; Molecular function:deoxyribonuclease V activity | |
| GO:0006281; Biological process:DNA repair | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| Pfam | PF04493; Endonuclease_5; 1; |
Features
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| From: NFI_THEMA (Q9X2H9) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 43 | 43 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 110 | 110 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[DEN] | 1 | |||||||
| SITE | 80 | 80 | /note="Interaction with target DNA" | Y | ||||||||
Additional information
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| Size range | 191-290 amino acids |
| Related rules |
None |
| Fusion | Nter: <Methyltransferase> Cter: None |
| Comments | Some archaea are bifunctional and contain a N-terminal methyltransferase domain. |