HAMAP rule MF_00802

General rule information [?]

Accession	MF_00802
Dates	21-DEC-2004 (Created) 26-JAN-2023 (Last updated, Version 20)

Name

GlnE

Scope

Bacteria; Actinomycetota

Bacteria; Pseudomonadota

Template

P30870 (GLNE_ECOLI)

Triggered by

HAMAP; MF_00802 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

GLNE

Protein name

RecName:		Full=Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme;
AltName:		Full=ATP:glutamine synthetase adenylyltransferase;
AltName:		Full=ATase;
Includes:
	RecName:		Full=Glutamine synthetase adenylyl-L-tyrosine phosphorylase;
			EC 2.7.7.89;
	AltName:		Full=Adenylyl removase;
			Short=AR;
			Short=AT-N;
Includes:
	RecName:		Full=Glutamine synthetase adenylyl transferase;
			EC 2.7.7.42;
	AltName:		Full=Adenylyl transferase;
			Short=AT;
			Short=AT-C;

Gene name

glnE

Comments [?]

Function	Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.
Catalytic activity	RHEA:43716: [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP EC 2.7.7.89
	RHEA:18589: [glutamine synthetase]-L-tyrosine + ATP = [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + diphosphate EC 2.7.7.42
Cofactor	Mg(2+)
Similarity	Belongs to the GlnE family.

Keywords [?]

ATP-binding
Magnesium
Multifunctional enzyme
Nucleotide-binding
Nucleotidyltransferase
Transferase

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0008882; Molecular function: [glutamate-ammonia-ligase] adenylyltransferase activity.
GO:0000820; Biological process: regulation of glutamine family amino acid metabolic process.

Cross-references [?]

Pfam	PF03710; GlnE; 1;

Features [?]

From: GLNE_ECOLI (P30870)

Key From To Description Tag Condition FTGroup

REGION Nter 440 Adenylyl removase

REGION 449 Cter Adenylyl transferase

Additional information [?]

Size range	894-1076 amino acids
Related rules	None
Fusion	None

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