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Annotation rule MF_00802
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General rule information [?]

Accession MF_00802
Dates 21-DEC-2004 (Created)
18-NOV-2019 (Last updated, Version 18)
Name GlnE
Scope
Bacteria; Actinobacteria
Bacteria; Proteobacteria
Template P30870 (GLNE_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GLNE
Protein name
RecName: Full=Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme;
AltName: Full=ATP:glutamine synthetase adenylyltransferase;
AltName: Full=ATase;
Includes:
RecName: Full=Glutamine synthetase adenylyl-L-tyrosine phosphorylase;
EC 2.7.7.89;
AltName: Full=Adenylyl removase;
Short=AR;
Short=AT-N;
Includes:
RecName: Full=Glutamine synthetase adenylyl transferase;
EC 2.7.7.42;
AltName: Full=Adenylyl transferase;
Short=AT;
Short=AT-C;
Gene name
glnE

Comments [?]

Function Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.
Catalytic activity RHEA:43716: [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP
EC 2.7.7.89
RHEA:18589: [glutamine synthetase]-L-tyrosine + ATP = [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + diphosphate
EC 2.7.7.42
Cofactor Mg(2+)
Similarity Belongs to the GlnE family.

Keywords [?]


Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0008882; Molecular function: [glutamate-ammonia-ligase] adenylyltransferase activity.
GO:0000820; Biological process: regulation of glutamine family amino acid metabolic process.

Cross-references [?]

Pfam PF03710; GlnE; 1;

Features [?]

From: GLNE_ECOLI (P30870)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     440       Adenylyl removase        
REGION     449     Cter       Adenylyl transferase        

Additional information [?]

Size range 894-1076 amino acids
Related rules None
Fusion None