HAMAP rule MF_00802
General rule information
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| Accession | MF_00802 |
| Dates | 21-DEC-2004 (Created)
26-JAN-2023 (Last updated, Version 20) |
| Name | GlnE |
| Scope(s) |
Bacteria Actinomycetota Pseudomonadota |
| Template(s) | P30870 (GLNE_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00802 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GLNE |
| Protein name | RecName: Full=Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme; AltName: Full=ATP:glutamine synthetase adenylyltransferase; AltName: Full=ATase; Includes: RecName: Full=Glutamine synthetase adenylyl-L-tyrosine phosphorylase; EC=2.7.7.89; AltName: Full=Adenylyl removase; Short=AR; Short=AT-N; Includes: RecName: Full=Glutamine synthetase adenylyl transferase; EC=2.7.7.42; AltName: Full=Adenylyl transferase; Short=AT; Short=AT-C; |
| Gene name | Name=glnE; |
Comments
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| FUNCTION | Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell. |
| CATALYTIC ACTIVITY | Reaction=[glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP; Xref=Rhea:RHEA:43716, Rhea:RHEA-COMP:10660, Rhea:RHEA-COMP:10661, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858, ChEBI:CHEBI:83624, ChEBI:CHEBI:456216; EC=2.7.7.89; |
| CATALYTIC ACTIVITY | Reaction=[glutamine synthetase]-L-tyrosine + ATP = [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + diphosphate; Xref=Rhea:RHEA:18589, Rhea:RHEA-COMP:10660, Rhea:RHEA-COMP:10661, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:46858, ChEBI:CHEBI:83624; EC=2.7.7.42; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SIMILARITY | Belongs to the GlnE family. |
Keywords
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Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0005524; Molecular function:ATP binding |
| GO:0008882; Molecular function:[glutamate-ammonia-ligase] adenylyltransferase activity |
| GO:0000820; Biological process:regulation of glutamine family amino acid metabolic process |
Cross-references
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| Pfam | PF03710; GlnE; 1; |
Features
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| From: GLNE_ECOLI (P30870) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 440 | /note="Adenylyl removase" | |||||||||
| REGION | 449 | Cter | /note="Adenylyl transferase" | |||||||||
Additional information
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| Size range | 894-1076 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |