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HAMAP rule MF_00802

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General rule information [?]

Accession MF_00802
Dates 21-DEC-2004 (Created)
26-JAN-2023 (Last updated, Version 20)
Name GlnE
Scope(s) Bacteria
Template(s) P30870 (GLNE_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00802 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GLNE
Protein name RecName: Full=Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme;
AltName: Full=ATP:glutamine synthetase adenylyltransferase;
AltName: Full=ATase;
RecName: Full=Glutamine synthetase adenylyl-L-tyrosine phosphorylase;
AltName: Full=Adenylyl removase;
RecName: Full=Glutamine synthetase adenylyl transferase;
AltName: Full=Adenylyl transferase;
Gene name Name=glnE;

Comments [?]

FUNCTIONInvolved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.
CATALYTIC ACTIVITY Reaction=[glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP; Xref=Rhea:RHEA:43716, Rhea:RHEA-COMP:10660, Rhea:RHEA-COMP:10661, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858, ChEBI:CHEBI:83624, ChEBI:CHEBI:456216; EC=;
CATALYTIC ACTIVITY Reaction=[glutamine synthetase]-L-tyrosine + ATP = [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + diphosphate; Xref=Rhea:RHEA:18589, Rhea:RHEA-COMP:10660, Rhea:RHEA-COMP:10661, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:46858, ChEBI:CHEBI:83624; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SIMILARITYBelongs to the GlnE family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0005524; Molecular function:ATP binding
GO:0008882; Molecular function:[glutamate-ammonia-ligase] adenylyltransferase activity
GO:0000820; Biological process:regulation of glutamine family amino acid metabolic process

Cross-references [?]

Pfam PF03710; GlnE; 1;

Features [?]

From: GLNE_ECOLI (P30870)
Key From To Description Tag Condition FTGroup
REGION Nter 440 /note="Adenylyl removase"
REGION 449 Cter /note="Adenylyl transferase"

Additional information [?]

Size range 894-1076 amino acids
Related rules None
Fusion Nter: None Cter: None

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