Home  |  Contact
Annotation rule MF_00804
Send feedback

General rule information [?]

Accession MF_00804
Dates 20-JAN-2005 (Created)
12-JAN-2021 (Last updated, Version 22)
Name BADH
Scope
Bacteria; Proteobacteria
Templates P17445 (BETB_ECOLI); Q9HTJ1 (BETB_PSEAE); Q3JLL8 (BETB_BURP1): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
BETB
Protein name
RecName: Full=Betaine aldehyde dehydrogenase;
Short=BADH;
EC 1.2.1.8;
Gene name
betB

Comments [?]

Function Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the irreversible oxidation of betaine aldehyde to the corresponding acid.
Catalytic activity RHEA:15305: betaine aldehyde + H2O + NAD(+) = glycine betaine + 2 H(+) + NADH
EC 1.2.1.8
PhysiologicalDirection=left-to-right (RHEA:15306)
Cofactor K(+)
Note: Binds 2 potassium ions per subunit.
Pathway Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1.
Subunit Dimer of dimers.
Similarity Belongs to the aldehyde dehydrogenase family.

Keywords [?]

NAD
NADP
Oxidoreductase
Oxidation
Metal-binding
Potassium

Gene Ontology [?]

GO:0008802; Molecular function: betaine-aldehyde dehydrogenase activity.
GO:0019285; Biological process: glycine betaine biosynthetic process from choline.

Cross-references [?]

Pfam PF00171; Aldedh; 1;
TIGRFAMs TIGR01804; BADH; 1;
PROSITE PS00070; ALDEHYDE_DEHYDR_CYS; 1;
PS00687; ALDEHYDE_DEHYDR_GLU; 1;

Features [?]

From: BETB_ECOLI (P17445)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     150     152       NAD     G-A-W  
NP_BIND     176     179       NAD     K-P-S-E  
NP_BIND     230     233       NAD     G-x(2)-[ST]  
ACT_SITE     162     162       Charge relay system     K  
ACT_SITE     252     252       Proton acceptor     E  
ACT_SITE     286     286       Nucleophile     C  
ACT_SITE     464     464       Charge relay system     E  
METAL     26     26       Potassium 1     [TS]  
METAL     27     27       Potassium 1; via carbonyl oxygen     [IV]  
METAL     93     93       Potassium 1     [DN]  
METAL     180     180       Potassium 1; via carbonyl oxygen     I  
METAL     246     246       Potassium 2; via carbonyl oxygen     [LVI]  
METAL     457     457       Potassium 2; via carbonyl oxygen     K  
METAL     460     460       Potassium 2; via carbonyl oxygen     G  
BINDING     254     254       NAD; via amide nitrogen     G  
BINDING     286     286       NAD;covalent     C  
BINDING     387     387       NAD     E  
SITE     248     248       Seems to be a necessary countercharge to the potassium cations     [ED]  
MOD_RES     286     286       Cysteine sulfenic acid (-SOH)     C  

Additional information [?]

Size range 481-493 amino acids
Related rules None
Fusion None
Comments BetB from P.aeruginosa can use NADP(+) with similar efficiency to NAD(+), a property that can be used by the bacterium to produce the NADPH needed to combat the oxidative stress imposed by the host defenses.


View rule in raw text format (no links)