HAMAP rule MF_00817
General rule information
[?]
Accession | MF_00817 |
Dates | 8-NOV-2005 (Created) 26-JAN-2023 (Last updated, Version 26) |
Name | QueF_type2 |
Scope | Bacteria; Pseudomonadota |
Templates | Q46920 (QUEF_ECOLI); Q9KTK0 (QUEF_VIBCH): [Recover all] |
Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Identifier |
|
Protein name |
|
Gene name |
|
Comments
[?]
Function | Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). |
Catalytic activity | RHEA:13409: 7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-deazaguanine + 3 H(+) + 2 NADPH
EC 1.7.1.13 |
Subunit | Homodimer. |
Pathway | tRNA modification; tRNA-queuosine biosynthesis. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily. |
Keywords
[?]
Gene Ontology
[?]
GO:0033739; Molecular function: preQ1 synthase activity.
GO:0008616; Biological process: queuosine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
GO:0008616; Biological process: queuosine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
Cross-references
[?]
Pfam | PF01227; GTP_cyclohydroI; 1; |
PIRSF | PIRSF004750; Nitrile_oxidored_YqcD_prd; 1; |
TIGRFAMs | TIGR03138; QueF; 1; |
Features
[?]
From: QUEF_VIBCH (Q9KTK0) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 89 | 90 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783 | S-[KQ] | ||||||||
BINDING | 256 | 257 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783 | R-G | ||||||||
BINDING | 87 | 89 | /ligand="substrate | [IV]-E-S | ||||||||
BINDING | 227 | 228 | /ligand="substrate | [HA]-E | ||||||||
ACT_SITE | 188 | 188 | Thioimide intermediate | C | ||||||||
ACT_SITE | 195 | 195 | Proton donor | D |
Additional information
[?]
Size range | 262-289 amino acids |
Related rules | MF_00818 (QUEF) |
Fusion | None |