HAMAP rule MF_00817

General rule information [?]

Accession	MF_00817
Dates	8-NOV-2005 (Created) 26-JAN-2023 (Last updated, Version 26)

Name	QueF_type2

Scope

Bacteria; Pseudomonadota

Templates

Q46920 (QUEF_ECOLI); Q9KTK0 (QUEF_VIBCH): [Recover all]

Triggered by

HAMAP; MF_00817 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

QUEF

Protein name

RecName:		Full=NADPH-dependent 7-cyano-7-deazaguanine reductase;
		EC 1.7.1.13;
AltName:		Full=7-cyano-7-carbaguanine reductase;
AltName:		Full=NADPH-dependent nitrile oxidoreductase;
AltName:		Full=PreQ(0) reductase;

Gene name

queF

Comments [?]

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
Catalytic activity	RHEA:13409: 7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-deazaguanine + 3 H(+) + 2 NADPH EC 1.7.1.13
Subunit	Homodimer.
Pathway	tRNA modification; tRNA-queuosine biosynthesis.
Subcellular location	Cytoplasm.
Similarity	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Keywords [?]

Cytoplasm
NADP
Oxidoreductase
Queuosine biosynthesis

Gene Ontology [?]

GO:0033739; Molecular function: preQ1 synthase activity.
GO:0008616; Biological process: queuosine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF01227; GTP_cyclohydroI; 1;
PIRSF	PIRSF004750; Nitrile_oxidored_YqcD_prd; 1;
TIGRFAMs	TIGR03138; QueF; 1;

Features [?]

From: QUEF_VIBCH (Q9KTK0)

Key From To Description Tag Condition FTGroup

BINDING 89 90 /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783 S-[KQ]

BINDING 256 257 /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783 R-G

BINDING 87 89 /ligand="substrate [IV]-E-S

BINDING 227 228 /ligand="substrate [HA]-E

ACT_SITE 188 188 Thioimide intermediate C

ACT_SITE 195 195 Proton donor D

Additional information [?]

Size range	262-289 amino acids
Related rules	MF_00818 (QUEF)
Fusion	None

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