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HAMAP rule MF_00817

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General rule information [?]

Accession MF_00817
Dates 8-NOV-2005 (Created)
26-JAN-2023 (Last updated, Version 26)
Name QueF_type2
Bacteria; Pseudomonadota
Templates Q46920 (QUEF_ECOLI); Q9KTK0 (QUEF_VIBCH): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase;
AltName: Full=7-cyano-7-carbaguanine reductase;
AltName: Full=NADPH-dependent nitrile oxidoreductase;
AltName: Full=PreQ(0) reductase;
Gene name

Comments [?]

Function Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
Catalytic activity RHEA:13409: 7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-deazaguanine + 3 H(+) + 2 NADPH
Subunit Homodimer.
Pathway tRNA modification; tRNA-queuosine biosynthesis.
Subcellular location Cytoplasm.
Similarity Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0033739; Molecular function: preQ1 synthase activity.
GO:0008616; Biological process: queuosine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01227; GTP_cyclohydroI; 1;
PIRSF PIRSF004750; Nitrile_oxidored_YqcD_prd; 1;
TIGRFAMs TIGR03138; QueF; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
BINDING     89     90       /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783     S-[KQ]  
BINDING     256     257       /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783     R-G  
BINDING     87     89       /ligand="substrate     [IV]-E-S  
BINDING     227     228       /ligand="substrate     [HA]-E  
ACT_SITE     188     188       Thioimide intermediate     C  
ACT_SITE     195     195       Proton donor     D  

Additional information [?]

Size range 262-289 amino acids
Related rules MF_00818 (QUEF)
Fusion None