HAMAP rule MF_00817
General rule information
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| Accession | MF_00817 |
| Dates | 8-NOV-2005 (Created)
3-SEP-2024 (Last updated, Version 29) |
| Name | QueF_type2 |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | Q46920 (QUEF_ECOLI); Q9KTK0 (QUEF_VIBCH); [ Recover all ] |
| Triggered by |
HAMAP; MF_00817 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | QUEF |
| Protein name | RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase; EC=1.7.1.13; AltName: Full=7-cyano-7-carbaguanine reductase; AltName: Full=NADPH-dependent nitrile oxidoreductase; AltName: Full=PreQ(0) reductase; |
| Gene name | Name=queF; |
Comments
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| FUNCTION | Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). |
| CATALYTIC ACTIVITY | Reaction=7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7- deazaguanine + 2 NADPH + 3 H(+); Xref=Rhea:RHEA:13409, ChEBI:CHEBI:15378, ChEBI:CHEBI:45075, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58703; EC=1.7.1.13; |
| SUBUNIT | Homodimer. |
| PATHWAY | tRNA modification; tRNA-queuosine biosynthesis. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily. |
Keywords
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Gene Ontology
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| GO:0033739; Molecular function:preQ1 synthase activity |
| GO:0008616; Biological process:queuosine biosynthetic process |
| GO:0006400; Biological process:tRNA modification |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF01227; GTP_cyclohydroI; 1; |
| PIRSF | PIRSF004750; Nitrile_oxidored_YqcD_prd; 1; |
| NCBIfam | TIGR03138; QueF; 1; |
Features
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| From: QUEF_VIBCH (Q9KTK0) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 89 | 90 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
S-[KQ] | ||||||||
| BINDING | 256 | 257 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
R-G | ||||||||
| BINDING | 87 | 89 | /ligand="substrate" | [IV]-E-S | ||||||||
| BINDING | 227 | 228 | /ligand="substrate" | [HA]-E | ||||||||
| ACT_SITE | 188 | 188 | /note="Thioimide intermediate" | C | ||||||||
| ACT_SITE | 195 | 195 | /note="Proton donor" | D | ||||||||
Additional information
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| Size range | 262-289 amino acids |
| Related rules |
MF_00818 |
| Fusion | Nter: None Cter: None |