HAMAP logo

HAMAP rule MF_00817

Send feedback

General rule information [?]

Accession MF_00817
Dates 8-NOV-2005 (Created)
31-JAN-2024 (Last updated, Version 28)
Name QueF_type2
Scope(s) Bacteria
Template(s) Q46920 (QUEF_ECOLI); Q9KTK0 (QUEF_VIBCH); [ Recover all ]
Triggered by HAMAP; MF_00817 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier QUEF
Protein name RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase;
AltName: Full=7-cyano-7-carbaguanine reductase;
AltName: Full=NADPH-dependent nitrile oxidoreductase;
AltName: Full=PreQ(0) reductase;
Gene name Name=queF;

Comments [?]

FUNCTIONCatalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
CATALYTIC ACTIVITY Reaction=7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7- deazaguanine + 3 H(+) + 2 NADPH; Xref=Rhea:RHEA:13409, ChEBI:CHEBI:15378, ChEBI:CHEBI:45075, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58703; EC=;
PATHWAYtRNA modification; tRNA-queuosine biosynthesis.
SIMILARITYBelongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0033739; Molecular function:preQ1 synthase activity
GO:0008616; Biological process:queuosine biosynthetic process
GO:0006400; Biological process:tRNA modification
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01227; GTP_cyclohydroI; 1;
PIRSF PIRSF004750; Nitrile_oxidored_YqcD_prd; 1;
NCBIfam TIGR03138; QueF; 1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 89 90 /ligand="NADPH"
BINDING 256 257 /ligand="NADPH"
BINDING 87 89 /ligand="substrate" [IV]-E-S
BINDING 227 228 /ligand="substrate" [HA]-E
ACT_SITE 188 188 /note="Thioimide intermediate" C
ACT_SITE 195 195 /note="Proton donor" D

Additional information [?]

Size range 262-289 amino acids
Related rules MF_00818
Fusion Nter: None Cter: None

View rule in raw text format (no links)