HAMAP rule MF_00820

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00820
Accession	MF_00820
Dates	29-NOV-2005 (Created) 03-SEP-2024 (Last updated, Version 18)
Name	Diketo_gul_reduc
Scope(s)	Bacteria Gammaproteobacteria
Template(s)	P37672; [ Recover all ]
Triggered by	HAMAP; MF_00820 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	DLGD
Protein name	RecName: Full=2,3-diketo-L-gulonate reductase; Short=2,3-DKG reductase; EC=1.1.1.130; AltName: Full=3-dehydro-L-gulonate 2-dehydrogenase;
Gene name	Name=dlgD;

Comments [?]

FUNCTION	Catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH, to form 3-keto-L-gulonate.
CATALYTIC ACTIVITY	Reaction=3-dehydro-L-gulonate + NAD(+) = 2,3-dioxo-L-gulonate + NADH + H(+); Xref=Rhea:RHEA:21924, ChEBI:CHEBI:15378, ChEBI:CHEBI:57441, ChEBI:CHEBI:57540, ChEBI:CHEBI:57655, ChEBI:CHEBI:57945; EC=1.1.1.130;
CATALYTIC ACTIVITY	Reaction=3-dehydro-L-gulonate + NADP(+) = 2,3-dioxo-L-gulonate + NADPH + H(+); Xref=Rhea:RHEA:21928, ChEBI:CHEBI:15378, ChEBI:CHEBI:57441, ChEBI:CHEBI:57655, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.130;
SUBUNIT	Homodimer.
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the LDH2/MDH2 oxidoreductase family. DlgD subfamily.

Keywords [?]

Gene Ontology [?]

GO:0047559; Molecular function:3-dehydro-L-gulonate 2-dehydrogenase activity

GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam	PF02615; Ldh_2; 1;

Features [?]

From: DLGD_ECOLI (P37672)

Key

From

Description

Tag

Condition

FTGroup

BINDING

168

174

/ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"

x-x-M-x-D-x-S

BINDING

224

225

/ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"

W-K

BINDING

304

306

/ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"

G-x-E

ACT_SITE

/note="Proton donor"

Additional information [?]