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HAMAP rule MF_00826

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General rule information [?]

Accession MF_00826
Dates 1-SEP-2006 (Created)
5-MAY-2020 (Last updated, Version 17)
Name GRDA
Scope(s) Bacteria
Template(s) P26971 (GRDA_ACESD); P52216 (GRDA_PEPLI); P50972 (GRDA1_PEPAC); [ Recover all ]
Triggered by HAMAP; MF_00826 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GRDA
Protein name RecName: Full=Glycine/sarcosine/betaine reductase complex component A;
                 EC=1.21.4.2;
                 EC=1.21.4.3;
                 EC=1.21.4.4;
AltName: Full=Selenoprotein PA;
AltName: Full=Thioredoxin reductase complex selenoprotein A;
Gene name Name=grdA;

Comments [?]

FUNCTIONIn the first step of glycine, betaine and sarcosine reductases, the substrate is bound to component PB via a Schiff base intermediate. Then the PB-activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated selenoether and the respective amine. By action of component PC, acetyl phosphate is formed, leaving component PA in its oxidized state. Finally component PA becomes reduced by the thioredoxin system to start a new catalytic cycle of reductive deamination.
CATALYTIC ACTIVITY Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + NH4(+) = [thioredoxin]-dithiol + glycine + H(+) + phosphate; Xref=Rhea:RHEA:12232, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:22191, ChEBI:CHEBI:28938, ChEBI:CHEBI:29950, ChEBI:CHEBI:43474, ChEBI:CHEBI:50058, ChEBI:CHEBI:57305; EC=1.21.4.2;
CATALYTIC ACTIVITY Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + methylamine = [thioredoxin]-dithiol + H(+) + phosphate + sarcosine; Xref=Rhea:RHEA:12825, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:22191, ChEBI:CHEBI:29950, ChEBI:CHEBI:43474, ChEBI:CHEBI:50058, ChEBI:CHEBI:57433, ChEBI:CHEBI:59338; EC=1.21.4.3;
CATALYTIC ACTIVITY Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + trimethylamine = [thioredoxin]-dithiol + glycine betaine + H(+) + phosphate; Xref=Rhea:RHEA:11848, Rhea:RHEA-COMP:10698, Rhea:RHEA- COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17750, ChEBI:CHEBI:22191, ChEBI:CHEBI:29950, ChEBI:CHEBI:43474, ChEBI:CHEBI:50058, ChEBI:CHEBI:58389; EC=1.21.4.4;
SUBUNITMonomer. Component of the glycine, sarcosine and betaine reductase complexes, together with components B and C.
SIMILARITYBelongs to the GrdA family.

Keywords [?]


Gene Ontology [?]

GO:0030699; Molecular function:glycine reductase activity
GO:0050485; Molecular function:oxidoreductase activity, acting on X-H and Y-H to form an X-Y bond, with a disulfide as acceptor

Cross-references [?]

Pfam PF04723; GRDA; 1;

Features [?]

From: GRDA_ACESD (P26971)
Key From To Description Tag Condition FTGroup
ACT_SITE 44 44 [CU]

Additional information [?]

Size range 150-161 amino acids
Related rules None
Fusion Nter: None Cter: None



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