HAMAP rule MF_00826
General rule information
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| Accession | MF_00826 |
| Dates | 1-SEP-2006 (Created)
5-MAY-2020 (Last updated, Version 17) |
| Name | GRDA |
| Scope(s) |
Bacteria |
| Template(s) | P26971 (GRDA_ACESD); P52216 (GRDA_PEPLI); P50972 (GRDA1_PEPAC); [ Recover all ] |
| Triggered by |
HAMAP; MF_00826 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GRDA |
| Protein name | RecName: Full=Glycine/sarcosine/betaine reductase complex component A; EC=1.21.4.2; EC=1.21.4.3; EC=1.21.4.4; AltName: Full=Selenoprotein PA; AltName: Full=Thioredoxin reductase complex selenoprotein A; |
| Gene name | Name=grdA; |
Comments
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| FUNCTION | In the first step of glycine, betaine and sarcosine reductases, the substrate is bound to component PB via a Schiff base intermediate. Then the PB-activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated selenoether and the respective amine. By action of component PC, acetyl phosphate is formed, leaving component PA in its oxidized state. Finally component PA becomes reduced by the thioredoxin system to start a new catalytic cycle of reductive deamination. |
| CATALYTIC ACTIVITY | Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + NH4(+) = [thioredoxin]-dithiol + glycine + H(+) + phosphate; Xref=Rhea:RHEA:12232, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:22191, ChEBI:CHEBI:28938, ChEBI:CHEBI:29950, ChEBI:CHEBI:43474, ChEBI:CHEBI:50058, ChEBI:CHEBI:57305; EC=1.21.4.2; |
| CATALYTIC ACTIVITY | Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + methylamine = [thioredoxin]-dithiol + H(+) + phosphate + sarcosine; Xref=Rhea:RHEA:12825, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:22191, ChEBI:CHEBI:29950, ChEBI:CHEBI:43474, ChEBI:CHEBI:50058, ChEBI:CHEBI:57433, ChEBI:CHEBI:59338; EC=1.21.4.3; |
| CATALYTIC ACTIVITY | Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + trimethylamine = [thioredoxin]-dithiol + glycine betaine + H(+) + phosphate; Xref=Rhea:RHEA:11848, Rhea:RHEA-COMP:10698, Rhea:RHEA- COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17750, ChEBI:CHEBI:22191, ChEBI:CHEBI:29950, ChEBI:CHEBI:43474, ChEBI:CHEBI:50058, ChEBI:CHEBI:58389; EC=1.21.4.4; |
| SUBUNIT | Monomer. Component of the glycine, sarcosine and betaine reductase complexes, together with components B and C. |
| SIMILARITY | Belongs to the GrdA family. |
Keywords
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Gene Ontology
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| GO:0030699; Molecular function:glycine reductase activity |
| GO:0050485; Molecular function:oxidoreductase activity, acting on X-H and Y-H to form an X-Y bond, with a disulfide as acceptor |
Cross-references
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| Pfam | PF04723; GRDA; 1; |
Features
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| From: GRDA_ACESD (P26971) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 44 | 44 | [CU] | |||||||||
Additional information
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| Size range | 150-161 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |