HAMAP rule MF_00846
General rule information
[?]
| Accession | MF_00846 |
| Dates | 18-NOV-2019 (Created) 17-FEB-2023 (Last updated, Version 6) |
| Name | VmlR |
| Scope | Bacteria; Actinomycetota
Bacteria; Bacillota
Bacteria; Mycoplasmatota |
| Template | P39115 (VMLR_BACSU) |
| Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier |
|
| Protein name |
|
||||
| Gene name |
|
Comments
[?]
| Function | Recognizes and binds in the vacant E-site of ribosomes stalled by some peptidyltransferase center (PTC)-targeting antibiotics. Makes contact with the PTC and both ribosomal subunits. Induces conformational changes in the P-site, which allows it to dislodge the antibiotic from its PTC binding site. |
| Subunit | Binds within the E-site of the 70S ribosome, where it contacts ribosomal proteins of the large and small subunit, the 16 and 23S rRNAs and the acceptor arm of the P-site tRNA. |
| Subcellular location | Cytoplasm. Note=Does not stably associate with ribosomes. |
| Domain | The antibiotic resistance domain (ARD) is packed between the 23S rRNA and the acceptor arm of the P-site tRNA and inserts into the peptidyltransferase center (PTC). The C-terminal extension (CTE) contacts the small ribosomal subunit, positioned in the Shine-Dalgarno-anti-Shine-Dalgarno cavity. |
| Similarity | Belongs to the ABC transporter superfamily. ABCF family. ARE2 subfamily. |
Keywords
[?]
Antibiotic resistance
ATP-binding
Cytoplasm
Nucleotide-binding
Repeat
RNA-binding
rRNA-binding
tRNA-binding
ATP-binding
Cytoplasm
Nucleotide-binding
Repeat
RNA-binding
rRNA-binding
tRNA-binding
Gene Ontology
[?]
GO:0005524; Molecular function: ATP binding.
GO:0005737; Cellular component: cytoplasm.
GO:0072344; Biological process: rescue of stalled ribosome.
GO:0019843; Molecular function: rRNA binding.
GO:0000049; Molecular function: tRNA binding.
GO:0005737; Cellular component: cytoplasm.
GO:0072344; Biological process: rescue of stalled ribosome.
GO:0019843; Molecular function: rRNA binding.
GO:0000049; Molecular function: tRNA binding.
Cross-references
[?]
Computed features
[?]
| General | Coiled_coil; -; 0-unlimited; trigger=yes; |
Features
[?]
| From: VMLR_BACSU (P39115) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | 5 | 200 | ABC transporter 1 | |||||||||
| DOMAIN | 292 | 504 | ABC transporter 2 | |||||||||
| BINDING | 37 | 44 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_label="1 | G-x-N-G-[ATV]-G-K-[ST] | ||||||||
| BINDING | 324 | 331 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_label="2 | G-x-N-G-[SICA]-G-K-[TS] | ||||||||
| REGION | 183 | 289 | Antibiotic resistance domain (ARD) | |||||||||
| REGION | 483 | 547 | C-terminal extension (CTE) | |||||||||
Additional information
[?]
| Size range | 530-560 amino acids |
| Related rules | None |
| Fusion | None |