HAMAP logo

HAMAP rule MF_00846

Send feedback

General rule information [?]

Accession MF_00846
Dates 18-NOV-2019 (Created)
17-FEB-2023 (Last updated, Version 6)
Name VmlR
Scope(s) Bacteria
Template(s) P39115 (VMLR_BACSU); [ Recover all ]
Triggered by HAMAP; MF_00846 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier VMLR
Protein name RecName: Full=Ribosome protection protein VmlR;
Gene name Name=vmlR;

Comments [?]

FUNCTIONRecognizes and binds in the vacant E-site of ribosomes stalled by some peptidyltransferase center (PTC)-targeting antibiotics. Makes contact with the PTC and both ribosomal subunits. Induces conformational changes in the P-site, which allows it to dislodge the antibiotic from its PTC binding site.
SUBUNITBinds within the E-site of the 70S ribosome, where it contacts ribosomal proteins of the large and small subunit, the 16 and 23S rRNAs and the acceptor arm of the P-site tRNA.
SUBCELLULAR LOCATIONCytoplasm. Note=Does not stably associate with ribosomes.
DOMAINThe antibiotic resistance domain (ARD) is packed between the 23S rRNA and the acceptor arm of the P-site tRNA and inserts into the peptidyltransferase center (PTC). The C-terminal extension (CTE) contacts the small ribosomal subunit, positioned in the Shine-Dalgarno- anti-Shine-Dalgarno cavity.
SIMILARITYBelongs to the ABC transporter superfamily. ABCF family. ARE2 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0005737; Cellular component:cytoplasm
GO:0072344; Biological process:rescue of stalled ribosome
GO:0019843; Molecular function:rRNA binding
GO:0000049; Molecular function:tRNA binding

Cross-references [?]

Pfam PF00005; ABC_tran; 3;
Pfam PF12848; ABC_tran_Xtn; 1;
General Coiled_coil; -; 0-unlimited;

Features [?]

From: VMLR_BACSU (P39115)
Key From To Description Tag Condition FTGroup
DOMAIN 5 200 /note="ABC transporter 1"
DOMAIN 292 504 /note="ABC transporter 2"
BINDING 37 44 /ligand="ATP"
BINDING 324 331 /ligand="ATP"
REGION 183 289 /note="Antibiotic resistance domain (ARD)"
REGION 483 547 /note="C-terminal extension (CTE)"

Additional information [?]

Size range 530-560 amino acids
Related rules None
Fusion Nter: None Cter: None

View rule in raw text format (no links)