HAMAP rule MF_00847

General rule information [?]

Accession	MF_00847
Dates	26-NOV-2019 (Created) 1-JUN-2023 (Last updated, Version 8)

Name

EttA

Scope

Bacteria

Template

P0A9W3 (ETTA_ECOLI)

Triggered by

HAMAP; MF_00847 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

ETTA

Protein name

RecName:		Full=Energy-dependent translational throttle protein EttA;
		EC 3.6.1.-;
AltName:		Full=Translational regulatory factor EttA;

Gene name

ettA

Comments [?]

Function	A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. ATP hydrolysis probably frees it from the ribosome, which can enter the elongation phase.
Catalytic activity	RHEA:13065: ATP + H2O = ADP + H(+) + phosphate
Subunit	Monomer. Probably contacts ribosomal proteins L1, L5, L33 and S7, the 16S and 23S rRNA and the P-site containing tRNA(fMet).
Subcellular location	Cytoplasm. Note=Associates with ribosomes and polysomes.
Domain	The arm domain is inserted in the first ABC transporter domain. Probably contacts ribosomal protein L1.
	The P-site tRNA interaction motif (PtIM domain) probably interacts with the P-site tRNA(fMet) as well as the 23S rRNA.
Similarity	Belongs to the ABC transporter superfamily. ABCF family. Translational throttle EttA subfamily.

Keywords [?]

ATP-binding
Cytoplasm
Hydrolase
Nucleotide-binding
Protein biosynthesis
Repeat
RNA-binding
rRNA-binding
Translation regulation
tRNA-binding

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0016887; Molecular function: ATP hydrolysis activity.
GO:0005737; Cellular component: cytoplasm.
GO:0019843; Molecular function: rRNA binding.
GO:0000049; Molecular function: tRNA binding.
GO:0043022; Molecular function: ribosome binding.
GO:0045900; Biological process: negative regulation of translational elongation.

Cross-references [?]

PROSITE	PS00211; ABC_TRANSPORTER_1; 1;
	PS50893; ABC_TRANSPORTER_2; 2;
Pfam	PF00005; ABC_tran; 2;
	PF12848; ABC_tran_Xtn; 1;
NCBIfam	TIGR03719; ABC_ABC_ChvD; 1;

Features [?]

From: ETTA_ECOLI (P0A9W3)

Key From To Description Tag Condition FTGroup

DOMAIN 6 259 ABC transporter 1

DOMAIN 324 550 ABC transporter 2

BINDING 39 46 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_label="1 G-x-N-G-[ATV]-G-K-[ST]

BINDING 356 363 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_label="2 G-x-N-G-[SICAV]-G-K-[TS]

REGION 95 139 Arm

REGION 242 322 PtIM

Additional information [?]

Size range	544-646 amino acids
Related rules	None
Fusion	None

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