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| Annotation rule MF_00847 |
General rule information
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| Accession | MF_00847 |
| Dates | 26-NOV-2019 (Created) 19-NOV-2022 (Last updated, Version 7) |
| Name | EttA |
| Scope | Bacteria |
| Template | P0A9W3 (ETTA_ECOLI) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. ATP hydrolysis probably frees it from the ribosome, which can enter the elongation phase. |
| Catalytic activity | RHEA:13065: ATP + H2O = ADP + H(+) + phosphate |
| Subunit | Monomer. Probably contacts ribosomal proteins L1, L5, L33 and S7, the 16S and 23S rRNA and the P-site containing tRNA(fMet). |
| Subcellular location | Cytoplasm. Note=Associates with ribosomes and polysomes. |
| Domain | The arm domain is inserted in the first ABC transporter domain. Probably contacts ribosomal protein L1. |
| The P-site tRNA interaction motif (PtIM domain) probably interacts with the P-site tRNA(fMet) as well as the 23S rRNA. | |
| Similarity | Belongs to the ABC transporter superfamily. ABCF family. Translational throttle EttA subfamily. |
Keywords
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ATP-binding
Cytoplasm
Hydrolase
Nucleotide-binding
Protein biosynthesis
Repeat
RNA-binding
rRNA-binding
Translation regulation
tRNA-binding
Cytoplasm
Hydrolase
Nucleotide-binding
Protein biosynthesis
Repeat
RNA-binding
rRNA-binding
Translation regulation
tRNA-binding
Gene Ontology
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GO:0005524; Molecular function: ATP binding.
GO:0016887; Molecular function: ATP hydrolysis activity.
GO:0005737; Cellular component: cytoplasm.
GO:0019843; Molecular function: rRNA binding.
GO:0000049; Molecular function: tRNA binding.
GO:0043022; Molecular function: ribosome binding.
GO:0045900; Biological process: negative regulation of translational elongation.
GO:0016887; Molecular function: ATP hydrolysis activity.
GO:0005737; Cellular component: cytoplasm.
GO:0019843; Molecular function: rRNA binding.
GO:0000049; Molecular function: tRNA binding.
GO:0043022; Molecular function: ribosome binding.
GO:0045900; Biological process: negative regulation of translational elongation.
Cross-references
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| PROSITE | PS00211; ABC_TRANSPORTER_1; 1; |
| PS50893; ABC_TRANSPORTER_2; 2; | |
| Pfam | PF00005; ABC_tran; 2; |
| PF12848; ABC_tran_Xtn; 1; | |
| TIGRFAMs | TIGR03719; ABC_ABC_ChvD; 1; |
Features
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| From: ETTA_ECOLI (P0A9W3) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | 6 | 259 | ABC transporter 1 | |||||||||
| DOMAIN | 324 | 550 | ABC transporter 2 | |||||||||
| BINDING | 39 | 46 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_label="1 | G-x-N-G-[ATV]-G-K-[ST] | ||||||||
| BINDING | 356 | 363 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_label="2 | G-x-N-G-[SICAV]-G-K-[TS] | ||||||||
| REGION | 95 | 139 | Arm | |||||||||
| REGION | 242 | 322 | PtIM | |||||||||
Additional information
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| Size range | 544-646 amino acids |
| Related rules | None |
| Fusion | None |