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HAMAP rule MF_00847

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General rule information [?]

Accession MF_00847
Dates 26-NOV-2019 (Created)
1-JUN-2023 (Last updated, Version 8)
Name EttA
Scope(s) Bacteria
Template(s) P0A9W3 (ETTA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00847 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ETTA
Protein name RecName: Full=Energy-dependent translational throttle protein EttA;
AltName: Full=Translational regulatory factor EttA;
Gene name Name=ettA;

Comments [?]

FUNCTIONA translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. ATP hydrolysis probably frees it from the ribosome, which can enter the elongation phase.
CATALYTIC ACTIVITY Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216;
SUBUNITMonomer. Probably contacts ribosomal proteins L1, L5, L33 and S7, the 16S and 23S rRNA and the P-site containing tRNA(fMet).
SUBCELLULAR LOCATIONCytoplasm. Note=Associates with ribosomes and polysomes.
DOMAINThe arm domain is inserted in the first ABC transporter domain. Probably contacts ribosomal protein L1.
DOMAINThe P-site tRNA interaction motif (PtIM domain) probably interacts with the P-site tRNA(fMet) as well as the 23S rRNA.
SIMILARITYBelongs to the ABC transporter superfamily. ABCF family. Translational throttle EttA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0016887; Molecular function:ATP hydrolysis activity
GO:0005737; Cellular component:cytoplasm
GO:0019843; Molecular function:rRNA binding
GO:0000049; Molecular function:tRNA binding
GO:0043022; Molecular function:ribosome binding
GO:0045900; Biological process:negative regulation of translational elongation

Cross-references [?]

Pfam PF00005; ABC_tran; 2;
Pfam PF12848; ABC_tran_Xtn; 1;
NCBIfam TIGR03719; ABC_ABC_ChvD; 1;

Features [?]

Key From To Description Tag Condition FTGroup
DOMAIN 6 259 /note="ABC transporter 1"
DOMAIN 324 550 /note="ABC transporter 2"
BINDING 39 46 /ligand="ATP"
BINDING 356 363 /ligand="ATP"
REGION 95 139 /note="Arm"
REGION 242 322 /note="PtIM"

Additional information [?]

Size range 544-646 amino acids
Related rules None
Fusion Nter: None Cter: None

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