HAMAP rule MF_00870
General rule information
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| Accession | MF_00870 |
| Dates | 19-FEB-2024 (Created)
4-MAR-2024 (Last updated, Version 2) |
| Name | FttA |
| Scope(s) |
Archaea |
| Template(s) | Q5JH24 (FTTA_THEKO); Q6LZD5 (FTTA_METMP); O27271 (FTTA_METTH); Q8PZ03 (FTTA_METMA); Q9V0P0 (FTTA_PYRAB); [ Recover all ] |
| Triggered by |
HAMAP; MF_00870 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | FTTA |
| Protein name | RecName: Full=Transcription termination factor FttA; EC=3.1.-.-; |
| Gene name | Name=fttA; |
Comments
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| FUNCTION | Terminates transcription on the whole genome. Termination is linked to FttA-mediated RNA cleavage and does not require NTP hydrolysis. Cleaves endonucleolytically at the RNA exit channel of RNA polymerase (RNAP); the 5'-3' exonuclease activity of this protein degrades the nascent RNA released from RNAP. |
| case <FTGroup:1> and <FTGroup:2> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions, which are required for nuclease activity. |
| end case | |
| SUBUNIT | Homodimer. Interacts with RNA polymerase (RNAP), interacts with the Spt4-Spt5 complex. |
| SIMILARITY | Belongs to the metallo-beta-lactamase superfamily. RNA- metabolizing metallo-beta-lactamase-like family. FttA subfamily. |
Keywords
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| DNA-binding | |
| Endonuclease | |
| Exonuclease | |
| Hydrolase | |
| Nuclease | |
| RNA-binding | |
| Transcription regulation | |
| Transcription termination | |
| case <FTGroup:1> or <FTGroup:2> | |
| Zinc | |
| Metal-binding | |
| end case | |
Gene Ontology
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| GO:0006353; Biological process:DNA-templated transcription termination | |
| GO:0003723; Molecular function:RNA binding | |
| GO:0004521; Molecular function:RNA endonuclease activity | |
| GO:0004532; Molecular function:RNA exonuclease activity | |
| case <FTGroup:1> or <FTGroup:2> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
Cross-references
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| PROSITE | PS50084; KH_TYPE_1; 0-1; |
| Pfam | PF00753; Lactamase_B; 0-1; |
| Pfam | PF07521; RMMBL; 1; |
| Pfam | PF07650; KH_2; 0-1; |
| Pfam | PF10996; Beta-Casp; 1; |
| Pfam | PF16661; Lactamase_B_6; 0-1; |
| Pfam | PF17214; KH_7; 1; |
| NCBIfam | TIGR03675; arCOG00543; 1; |
Features
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| From: FTTA_METTH (O27271) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 3 | 70 | /note="KHa" | |||||||||
| REGION | 71 | 138 | /note="KHb" | |||||||||
| REGION | 179 | 383 | /note="Metallo-beta-lactamase N-terminus" | |||||||||
| REGION | 384 | 577 | /note="Beta-Casp" | |||||||||
| REGION | 578 | 636 | /note="Metallo-beta-lactamase C-terminus" | |||||||||
| BINDING | 242 | 242 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
H | 1 | |||||||
| BINDING | 244 | 244 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
H | 1 | |||||||
| BINDING | 246 | 246 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
D | 2 | |||||||
| BINDING | 247 | 247 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
H | 2 | |||||||
| BINDING | 329 | 329 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
H | 1 | |||||||
| BINDING | 352 | 352 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
D | 1 | |||||||
| BINDING | 352 | 352 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
D | 2 | |||||||
| BINDING | 603 | 603 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
H | 2 | |||||||
Additional information
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| Size range | 634-651 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |