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HAMAP rule MF_00916

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General rule information [?]

Accession MF_00916
Dates 6-FEB-2012 (Created)
31-JAN-2024 (Last updated, Version 14)
Name QueG
Scope(s) Bacteria
Template(s) P97030 (QUEG_BACSU); P39288 (QUEG_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00916 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier QUEG
Protein name RecName: Full=Epoxyqueuosine reductase;
                 EC=1.17.99.6;
AltName: Full=Queuosine biosynthesis protein QueG;
Gene name Name=queG;

Comments [?]

FUNCTIONCatalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
CATALYTIC ACTIVITY Reaction=AH2 + epoxyqueuosine(34) in tRNA = A + H2O + queuosine(34) in tRNA; Xref=Rhea:RHEA:32159, Rhea:RHEA-COMP:18571, Rhea:RHEA- COMP:18582, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:17499, ChEBI:CHEBI:194431, ChEBI:CHEBI:194443; EC=1.17.99.6;
COFACTOR Name=cob(II)alamin; Xref=ChEBI:CHEBI:16304;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters per monomer.
PATHWAYtRNA modification; tRNA-queuosine biosynthesis.
SUBUNITMonomer. {ECO:0000269|PubMed:26230193}.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the QueG family.

Keywords [?]


Gene Ontology [?]

GO:0052693; Molecular function:epoxyqueuosine reductase activity
GO:0051536; Molecular function:iron-sulfur cluster binding
GO:0008616; Biological process:queuosine biosynthetic process
GO:0006400; Biological process:tRNA modification
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS00198; 4FE4S_FER_1; 1;
PROSITE PS51379; 4FE4S_FER_2; 1-2;
Pfam PF08331; DUF1730; 1;
Pfam PF03130; HEAT_PBS; 0-1;
NCBIfam TIGR00276; TIGR00276; 1;

Features [?]

From: QUEG_BACSU (P97030)
Key From To Description Tag Condition FTGroup
BINDING 139 141 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
S-[DE]-[RK]
BINDING 240 241 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
C-D
ACT_SITE 134 134 /note="Proton donor" D
BINDING 188 188 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C
BINDING 191 191 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C
BINDING 194 194 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C
BINDING 198 198 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C
BINDING 214 214 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C
BINDING 240 240 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C
BINDING 243 243 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C
BINDING 247 247 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C
BINDING 57 57 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
R
BINDING 97 97 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
C
BINDING 134 134 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
D
BINDING 152 152 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
[ST]
BINDING 155 155 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
N
BINDING 158 158 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
[ILV]
BINDING 169 169 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
[LI]
BINDING 216 216 /ligand="cob(II)alamin"
/ligand_id="ChEBI:CHEBI:16304"
S
BINDING 220 220 /ligand="tRNA"
/ligand_id="ChEBI:CHEBI:17843"
/ligand_part="tRNA anticodon stem loop"
Q
BINDING 222 222 /ligand="tRNA"
/ligand_id="ChEBI:CHEBI:17843"
/ligand_part="tRNA anticodon stem loop"
K
BINDING 280 280 /ligand="tRNA"
/ligand_id="ChEBI:CHEBI:17843"
/ligand_part="tRNA anticodon stem loop"
N
BINDING 281 281 /ligand="tRNA"
/ligand_id="ChEBI:CHEBI:17843"
/ligand_part="tRNA anticodon stem loop"
[RK]
BINDING 295 295 /ligand="tRNA"
/ligand_id="ChEBI:CHEBI:17843"
/ligand_part="tRNA anticodon stem loop"
R
BINDING 297 297 /ligand="tRNA"
/ligand_id="ChEBI:CHEBI:17843"
/ligand_part="tRNA anticodon stem loop"
K
BINDING 298 298 /ligand="tRNA"
/ligand_id="ChEBI:CHEBI:17843"
/ligand_part="tRNA anticodon stem loop"
K

Additional information [?]

Size range 289-411 amino acids
Related rules None
Fusion Nter: None Cter: None



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