HAMAP rule MF_00916
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00916 |
| Accession | MF_00916 |
| Dates | 06-FEB-2012 (Created)
12-JUN-2025 (Last updated, Version 18) |
| Name | QueG |
| Scope(s) |
Bacteria |
| Template(s) | P97030; P39288; [ Recover all ] |
| Triggered by |
HAMAP; MF_00916 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | QUEG |
| Protein name | RecName: Full=Epoxyqueuosine reductase; EC=1.17.99.6; AltName: Full=Queuosine biosynthesis protein QueG; |
| Gene name | Name=queG; |
Comments
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| FUNCTION | Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr). |
| CATALYTIC ACTIVITY | Reaction=epoxyqueuosine(34) in tRNA + AH2 = queuosine(34) in tRNA + A + H2O; Xref=Rhea:RHEA:32159, Rhea:RHEA-COMP:18571, Rhea:RHEA- COMP:18582, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:17499, ChEBI:CHEBI:194431, ChEBI:CHEBI:194443; EC=1.17.99.6; |
| COFACTOR | Name=cob(II)alamin; Xref=ChEBI:CHEBI:16304; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters per monomer. |
| PATHWAY | tRNA modification; tRNA-queuosine biosynthesis. |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the QueG family. |
Keywords
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| 4Fe-4S |
| Cobalamin |
| Cytoplasm |
| Iron |
| Iron-sulfur |
| Metal-binding |
| Oxidoreductase |
| Queuosine biosynthesis |
| tRNA processing |
Gene Ontology
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| GO:0052693; Molecular function:epoxyqueuosine reductase activity |
| GO:0051536; Molecular function:iron-sulfur cluster binding |
| GO:0008616; Biological process:tRNA queuosine(34) biosynthetic process |
| GO:0006400; Biological process:tRNA modification |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS00198; 4FE4S_FER_1; 1; |
| PROSITE | PS51379; 4FE4S_FER_2; 1-2; |
| Pfam | PF08331; DUF1730; 1; |
| Pfam | PF03130; HEAT_PBS; 0-1; |
| NCBIfam | TIGR00276; TIGR00276; 1; |
Features
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| From: QUEG_BACSU (P97030) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 139 | 141 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
S-[DE]-[RK] | ||||||||
| BINDING | 240 | 241 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
C-D | ||||||||
| ACT_SITE | 134 | 134 | /note="Proton donor" | D | ||||||||
| BINDING | 188 | 188 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 191 | 191 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 194 | 194 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 198 | 198 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" |
C | ||||||||
| BINDING | 214 | 214 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" |
C | ||||||||
| BINDING | 240 | 240 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" |
C | ||||||||
| BINDING | 243 | 243 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" |
C | ||||||||
| BINDING | 247 | 247 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 57 | 57 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
R | ||||||||
| BINDING | 97 | 97 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
C | ||||||||
| BINDING | 134 | 134 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
D | ||||||||
| BINDING | 152 | 152 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
[ST] | ||||||||
| BINDING | 155 | 155 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
N | ||||||||
| BINDING | 158 | 158 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
[ILV] | ||||||||
| BINDING | 169 | 169 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
[LI] | ||||||||
| BINDING | 216 | 216 | /ligand="cob(II)alamin" /ligand_id="ChEBI:CHEBI:16304" |
S | ||||||||
| BINDING | 220 | 220 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" /ligand_part="tRNA anticodon stem loop" |
Q | ||||||||
| BINDING | 222 | 222 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" /ligand_part="tRNA anticodon stem loop" |
K | ||||||||
| BINDING | 280 | 280 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" /ligand_part="tRNA anticodon stem loop" |
N | ||||||||
| BINDING | 281 | 281 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" /ligand_part="tRNA anticodon stem loop" |
[RK] | ||||||||
| BINDING | 295 | 295 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" /ligand_part="tRNA anticodon stem loop" |
R | ||||||||
| BINDING | 297 | 297 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" /ligand_part="tRNA anticodon stem loop" |
K | ||||||||
| BINDING | 298 | 298 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" /ligand_part="tRNA anticodon stem loop" |
K | ||||||||
Additional information
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| Size range | 289-411 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |