HAMAP logo
Due to maintenance work, this service will be unavailable on Monday between 06:00 until 06:30 CEST. Apologies for the inconvenience.

HAMAP rule MF_00934

Send feedback

General rule information [?]

Accession MF_00934
Dates 17-JAN-2013 (Created)
19-NOV-2022 (Last updated, Version 9)
Name 23SrRNA_methyltr_J
Scope(s) Bacteria
Template(s) P37634 (RLMJ_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00934 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RLMJ
Protein name RecName: Full=Ribosomal RNA large subunit methyltransferase J;
                 EC=2.1.1.266;
AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase;
AltName: Full=23S rRNA m6A2030 methyltransferase;
Gene name Name=rlmJ;

Comments [?]

FUNCTIONSpecifically methylates the adenine in position 2030 of 23S rRNA.
CATALYTIC ACTIVITY Reaction=adenosine(2030) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(6)-methyladenosine(2030) in 23S rRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43736, Rhea:RHEA-COMP:10668, Rhea:RHEA-COMP:10669, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.266;
SUBUNITMonomer.
SIMILARITYBelongs to the RlmJ family.

Keywords [?]


Gene Ontology [?]

GO:0036307; Molecular function:23S rRNA (adenine(2030)-N(6))-methyltransferase activity
GO:0003723; Molecular function:RNA binding
GO:0070475; Biological process:rRNA base methylation

Cross-references [?]

PROSITE PS00092; N6_MTASE; 2;
Pfam PF04378; DUF519; 1;

Features [?]

From: RLMJ_ECOLI (P37634)
Key From To Description Tag Condition FTGroup
BINDING 143 144 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[DNE]-[GAS]
ACT_SITE 164 164 /note="Proton acceptor" D
BINDING 19 19 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
H
BINDING 42 42 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[HYFN]
BINDING 100 100 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
S
BINDING 118 118 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[ED]
BINDING 164 164 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
D
SITE 4 4 /note="Interaction with substrate rRNA" Y

Additional information [?]

Size range 240-362 amino acids
Related rules None
Fusion Nter: None Cter: None



View rule in raw text format (no links)