HAMAP rule MF_00934
General rule information
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| Accession | MF_00934 |
| Dates | 17-JAN-2013 (Created)
3-SEP-2024 (Last updated, Version 10) |
| Name | 23SrRNA_methyltr_J |
| Scope(s) |
Bacteria |
| Template(s) | P37634 (RLMJ_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00934 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RLMJ |
| Protein name | RecName: Full=Ribosomal RNA large subunit methyltransferase J; EC=2.1.1.266; AltName: Full=23S rRNA (adenine(2030)-N6)-methyltransferase; AltName: Full=23S rRNA m6A2030 methyltransferase; |
| Gene name | Name=rlmJ; |
Comments
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| FUNCTION | Specifically methylates the adenine in position 2030 of 23S rRNA. |
| CATALYTIC ACTIVITY | Reaction=adenosine(2030) in 23S rRNA + S-adenosyl-L-methionine = N(6)- methyladenosine(2030) in 23S rRNA + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:43736, Rhea:RHEA-COMP:10668, Rhea:RHEA-COMP:10669, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.266; |
| SUBUNIT | Monomer. |
| SIMILARITY | Belongs to the RlmJ family. |
Keywords
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Gene Ontology
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| GO:0036307; Molecular function:23S rRNA (adenine(2030)-N(6))-methyltransferase activity |
| GO:0003723; Molecular function:RNA binding |
| GO:0070475; Biological process:rRNA base methylation |
Cross-references
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Features
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| From: RLMJ_ECOLI (P37634) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 143 | 144 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[DNE]-[GAS] | ||||||||
| ACT_SITE | 164 | 164 | /note="Proton acceptor" | D | ||||||||
| BINDING | 19 | 19 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
H | ||||||||
| BINDING | 42 | 42 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[HYFN] | ||||||||
| BINDING | 100 | 100 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
S | ||||||||
| BINDING | 118 | 118 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[ED] | ||||||||
| BINDING | 164 | 164 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | ||||||||
| SITE | 4 | 4 | /note="Interaction with substrate rRNA" | Y | ||||||||
Additional information
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| Size range | 240-362 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |