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HAMAP rule MF_00942

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General rule information [?]

Accession MF_00942
Dates 13-FEB-2013 (Created)
30-JAN-2024 (Last updated, Version 15)
Name Nth
Scope(s) Bacteria
Template(s) P0AB83 (END3_ECOLI); P9WQ11 (END3_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_00942 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier END3
Protein name RecName: Full=Endonuclease III;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase;
Gene name Name=nth;

Comments [?]

FUNCTIONDNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'- phosphate.
CATALYTIC ACTIVITY Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.;
SIMILARITYBelongs to the Nth/MutY family.

Keywords [?]

Gene Ontology [?]

GO:0019104; Molecular function:DNA N-glycosylase activity
GO:0003906; Molecular function:DNA-(apurinic or apyrimidinic site) endonuclease activity
GO:0003677; Molecular function:DNA binding
case <FTGroup:1>
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
end case
GO:0006281; Biological process:DNA repair

Cross-references [?]

Pfam PF10576; EndIII_4Fe-2S; 1;
Pfam PF00633; HHH; 1;
Pfam PF00730; HhH-GPD; 1;
NCBIfam TIGR01083; nth; 1;
PIRSF PIRSF001435; Nth; 1;

Features [?]

From: END3_ECOLI (P0AB83)
Key From To Description Tag Condition FTGroup
BINDING 187 187 /ligand="[4Fe-4S] cluster"
C 1
BINDING 194 194 /ligand="[4Fe-4S] cluster"
C 1
BINDING 197 197 /ligand="[4Fe-4S] cluster"
C 1
BINDING 203 203 /ligand="[4Fe-4S] cluster"
C 1

Additional information [?]

Size range 177-390 amino acids
Related rules None
Fusion Nter: None Cter: None

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