HAMAP rule MF_00942
General rule information
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Accession | MF_00942 |
Dates | 13-FEB-2013 (Created) 1-JUN-2023 (Last updated, Version 14) |
Name | Nth |
Scope | Bacteria
Archaea |
Templates | P0AB83 (END3_ECOLI); P9WQ11 (END3_MYCTU): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. |
Catalytic activity | RHEA:66592: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)
EC 4.2.99.18 |
Cofactor | [4Fe-4S] cluster Note: Binds 1 [4Fe-4S] cluster. |
Similarity | Belongs to the Nth/MutY family. |
Keywords
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case <FTGroup:1>
end case
Gene Ontology
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GO:0019104; Molecular function: DNA N-glycosylase activity.
GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0003677; Molecular function: DNA binding.
GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0003677; Molecular function: DNA binding.
case <FTGroup:1>
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
end case
GO:0006281; Biological process: DNA repair.
Cross-references
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PROSITE | PS00764; ENDONUCLEASE_III_1; 1; |
PS01155; ENDONUCLEASE_III_2; 1; | |
Pfam | PF10576; EndIII_4Fe-2S; 1; |
PF00633; HHH; 1; trigger=Yes; | |
PF00730; HhH-GPD; 1; | |
NCBIfam | TIGR01083; nth; 1; |
PIRSF | PIRSF001435; Nth; 1; |
Features
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From: END3_ECOLI (P0AB83) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 187 | 187 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 | |||||||
BINDING | 194 | 194 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 | |||||||
BINDING | 197 | 197 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 | |||||||
BINDING | 203 | 203 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 |
Additional information
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Size range | 177-390 amino acids |
Related rules | None |
Fusion | None |