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Annotation rule MF_00942
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General rule information [?]

Accession MF_00942
Dates 13-FEB-2013 (Created)
8-JUN-2021 (Last updated, Version 9)
Name Nth
Scope
Bacteria
Archaea
Templates P0AB83 (END3_ECOLI); P9WQ11 (END3_MYCTU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
END3
Protein name
RecName: Full=Endonuclease III;
EC 4.2.99.18;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase;
Gene name
nth

Comments [?]

Function DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Catalytic activity RHEA:66592: (DNA)-(deoxyribonucleoside 5'-phosphate)-(deoxyribose 5'-phosphate)-(deoxyribonucleoside 5'-phosphate) = (DNA)-3'-(deoxyribonucleoside 5'-phosphate)-(2,3-dehydro-2,3-deoxyribose 5'-phosphate) + H(+) + phospho-5'-(DNA)
EC 4.2.99.18
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster.
Similarity Belongs to the Nth/MutY family.

Keywords [?]

DNA-binding
DNA damage
DNA repair
Glycosidase
Hydrolase
Lyase
case <FTGroup:1>
4Fe-4S
Iron
Iron-sulfur
Metal-binding
end case

Gene Ontology [?]

GO:0019104; Molecular function: DNA N-glycosylase activity.
GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0003677; Molecular function: DNA binding.
case <FTGroup:1>
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
end case
GO:0006281; Biological process: DNA repair.

Cross-references [?]

PROSITE PS00764; ENDONUCLEASE_III_1; 1;
PS01155; ENDONUCLEASE_III_2; 1;
Pfam PF10576; EndIII_4Fe-2S; 1;
PF00633; HHH; 1; trigger=Yes;
PF00730; HhH-GPD; 1;
TIGRFAMs TIGR01083; nth; 1;
PIRSF PIRSF001435; Nth; 1;

Features [?]

From: END3_ECOLI (P0AB83)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     187     187       Iron-sulfur (4Fe-4S)     C   1
METAL     194     194       Iron-sulfur (4Fe-4S)     C   1
METAL     197     197       Iron-sulfur (4Fe-4S)     C   1
METAL     203     203       Iron-sulfur (4Fe-4S)     C   1

Additional information [?]

Size range 177-390 amino acids
Related rules None
Fusion None


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