HAMAP rule MF_00942

General rule information [?]

Accession	MF_00942
Dates	13-FEB-2013 (Created) 1-JUN-2023 (Last updated, Version 14)

Name

Nth

Scope

Bacteria

Archaea

Templates

P0AB83 (END3_ECOLI); P9WQ11 (END3_MYCTU): [Recover all]

Triggered by

HAMAP; MF_00942 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

END3

Protein name

RecName:		Full=Endonuclease III;
		EC 4.2.99.18;
AltName:		Full=DNA-(apurinic or apyrimidinic site) lyase;

Gene name

nth

Comments [?]

Function	DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Catalytic activity	RHEA:66592: 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+) EC 4.2.99.18
Cofactor	[4Fe-4S] cluster Note: Binds 1 [4Fe-4S] cluster.
Similarity	Belongs to the Nth/MutY family.

Keywords [?]

DNA-binding
DNA damage
DNA repair
Glycosidase
Hydrolase
Lyase

case <FTGroup:1>

4Fe-4S
Iron
Iron-sulfur
Metal-binding

end case

Gene Ontology [?]

GO:0019104; Molecular function: DNA N-glycosylase activity.
GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0003677; Molecular function: DNA binding.

case <FTGroup:1>

GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.

end case

GO:0006281; Biological process: DNA repair.

Cross-references [?]

PROSITE	PS00764; ENDONUCLEASE_III_1; 1;
	PS01155; ENDONUCLEASE_III_2; 1;
Pfam	PF10576; EndIII_4Fe-2S; 1;
	PF00633; HHH; 1; trigger=Yes;
	PF00730; HhH-GPD; 1;
NCBIfam	TIGR01083; nth; 1;
PIRSF	PIRSF001435; Nth; 1;

Features [?]

From: END3_ECOLI (P0AB83)

Key From To Description Tag Condition FTGroup

BINDING 187 187 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C 1

BINDING 194 194 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C 1

BINDING 197 197 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C 1

BINDING 203 203 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C 1

Additional information [?]

Size range	177-390 amino acids
Related rules	None
Fusion	None

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