HAMAP rule MF_00942
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00942 |
| Accession | MF_00942 |
| Dates | 13-FEB-2013 (Created)
30-JAN-2024 (Last updated, Version 15) |
| Name | Nth |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0AB83 (END3_ECOLI); P9WQ11 (END3_MYCTU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00942 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | END3 |
| Protein name | RecName: Full=Endonuclease III; EC=4.2.99.18; AltName: Full=DNA-(apurinic or apyrimidinic site) lyase; |
| Gene name | Name=nth; |
Comments
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| FUNCTION | DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'- phosphate. |
| CATALYTIC ACTIVITY | Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=4.2.99.18; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.; |
| SIMILARITY | Belongs to the Nth/MutY family. |
Keywords
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| DNA-binding | |
| DNA damage | |
| DNA repair | |
| Glycosidase | |
| Hydrolase | |
| Lyase | |
| case <FTGroup:1> | |
| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| end case | |
Gene Ontology
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| GO:0019104; Molecular function:DNA N-glycosylase activity | |
| GO:0003906; Molecular function:DNA-(apurinic or apyrimidinic site) endonuclease activity | |
| GO:0003677; Molecular function:DNA binding | |
| case <FTGroup:1> | |
| GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding | |
| end case | |
| GO:0006281; Biological process:DNA repair | |
Cross-references
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| PROSITE | PS00764; ENDONUCLEASE_III_1; 1; |
| PROSITE | PS01155; ENDONUCLEASE_III_2; 1; |
| Pfam | PF10576; EndIII_4Fe-2S; 1; |
| Pfam | PF00633; HHH; 1; |
| Pfam | PF00730; HhH-GPD; 1; |
| NCBIfam | TIGR01083; nth; 1; |
| PIRSF | PIRSF001435; Nth; 1; |
Features
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| From: END3_ECOLI (P0AB83) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 187 | 187 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 194 | 194 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 197 | 197 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 203 | 203 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
Additional information
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| Size range | 177-390 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |