HAMAP rule MF_00956
General rule information
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| Accession | MF_00956 |
| Dates | 12-APR-2013 (Created)
19-NOV-2022 (Last updated, Version 9) |
| Name | GDP_fucose_synth |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P32055 (FCL_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00956 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | FCL |
| Protein name | RecName: Full=GDP-L-fucose synthase; EC=1.1.1.271; AltName: Full=GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; |
| Gene name | Name=fcl; |
Comments
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| FUNCTION | Catalyzes the two-step NADP-dependent conversion of GDP-4- dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. |
| CATALYTIC ACTIVITY | Reaction=GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + H(+) + NADPH; Xref=Rhea:RHEA:18885, ChEBI:CHEBI:15378, ChEBI:CHEBI:57273, ChEBI:CHEBI:57783, ChEBI:CHEBI:57964, ChEBI:CHEBI:58349; EC=1.1.1.271; |
| PATHWAY | Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2. |
| SIMILARITY | Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily. |
Keywords
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Gene Ontology
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| GO:0050577; Molecular function:GDP-L-fucose synthase activity |
| GO:0070401; Molecular function:NADP+ binding |
| GO:0042351; Biological process:'de novo' GDP-L-fucose biosynthetic process |
Cross-references
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| Pfam | PF01370; Epimerase; 1; |
Features
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| From: FCL_ECOLI (P32055) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 10 | 16 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
G-x-x-G-x-x-G | ||||||||
| BINDING | 105 | 108 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
[MILV]-[GA]-[ST]-[ST] | ||||||||
| BINDING | 163 | 166 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
P-x-N-[IVL] | ||||||||
| ACT_SITE | 136 | 136 | /note="Proton donor/acceptor" | [YF] | ||||||||
| BINDING | 140 | 140 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
K | ||||||||
| BINDING | 179 | 179 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
H | ||||||||
| BINDING | 187 | 187 | /ligand="substrate" | [RK] | ||||||||
| BINDING | 202 | 202 | /ligand="substrate" | W | ||||||||
| BINDING | 209 | 209 | /ligand="substrate" | R | ||||||||
| BINDING | 278 | 278 | /ligand="substrate" | [DE] | ||||||||
| SITE | 107 | 107 | /note="Important for catalytic activity" | [ST] | ||||||||
| SITE | 109 | 109 | /note="Important for catalytic activity" | C | ||||||||
Additional information
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| Size range | 280-440 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |