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HAMAP rule MF_00956

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General rule information [?]

Accession MF_00956
Dates 12-APR-2013 (Created)
19-NOV-2022 (Last updated, Version 9)
Name GDP_fucose_synth
Scope(s) Bacteria
Template(s) P32055 (FCL_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00956 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier FCL
Protein name RecName: Full=GDP-L-fucose synthase;
AltName: Full=GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase;
Gene name Name=fcl;

Comments [?]

FUNCTIONCatalyzes the two-step NADP-dependent conversion of GDP-4- dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.
CATALYTIC ACTIVITY Reaction=GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + H(+) + NADPH; Xref=Rhea:RHEA:18885, ChEBI:CHEBI:15378, ChEBI:CHEBI:57273, ChEBI:CHEBI:57783, ChEBI:CHEBI:57964, ChEBI:CHEBI:58349; EC=;
PATHWAYNucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2.
SIMILARITYBelongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.

Keywords [?]

Gene Ontology [?]

GO:0050577; Molecular function:GDP-L-fucose synthase activity
GO:0070401; Molecular function:NADP+ binding
GO:0042351; Biological process:'de novo' GDP-L-fucose biosynthetic process

Cross-references [?]

Pfam PF01370; Epimerase; 1;

Features [?]

From: FCL_ECOLI (P32055)
Key From To Description Tag Condition FTGroup
BINDING 10 16 /ligand="NADP(+)"
BINDING 105 108 /ligand="NADP(+)"
BINDING 163 166 /ligand="NADP(+)"
ACT_SITE 136 136 /note="Proton donor/acceptor" [YF]
BINDING 140 140 /ligand="NADP(+)"
BINDING 179 179 /ligand="NADP(+)"
BINDING 187 187 /ligand="substrate" [RK]
BINDING 202 202 /ligand="substrate" W
BINDING 209 209 /ligand="substrate" R
BINDING 278 278 /ligand="substrate" [DE]
SITE 107 107 /note="Important for catalytic activity" [ST]
SITE 109 109 /note="Important for catalytic activity" C

Additional information [?]

Size range 280-440 amino acids
Related rules None
Fusion Nter: None Cter: None

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