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HAMAP rule MF_00963

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General rule information [?]

Accession MF_00963
Dates 31-MAY-2013 (Created)
1-JUN-2023 (Last updated, Version 21)
Name Sigma70_RpoD_SigA
Templates P00579 (RPOD_ECOLI); P0A0J0 (SIGA_STAA8); P77994 (SIGA_THEMA); Q72L95 (SIGA_THET2); Q9EZJ8 (SIGA_THEAQ): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Pseudomonadota> or <OC:Spirochaetales>
Protein name
RecName: Full=RNA polymerase sigma factor RpoD;
AltName: Full=Sigma-70;
Gene name
Protein name
RecName: Full=RNA polymerase sigma factor SigA;
Gene name
end case

Comments [?]

Function Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
Subunit Interacts transiently with the RNA polymerase catalytic core.
Subcellular location Cytoplasm.
Similarity Belongs to the sigma-70 factor family. RpoD/SigA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0016987; Molecular function: sigma factor activity.
GO:0010468; Biological process: regulation of gene expression.
GO:0006352; Biological process: DNA-templated transcription initiation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS00715; SIGMA70_1; 1;
PS00716; SIGMA70_2; 1;
Pfam PF04546; Sigma70_ner; 0-1;
PF03979; Sigma70_r1_1; 0-1;
PF00140; Sigma70_r1_2; 0-1;
PF04542; Sigma70_r2; 1;
PF04539; Sigma70_r3; 1;
PF04545; Sigma70_r4; 1;
NCBIfam TIGR02393; RpoD_Cterm; 1;
TIGR02937; sigma70-ECF; 1;
TIGR02997; Sig70-cyanoRpoD; 0-1;

Features [?]

From: RPOD_ECOLI (P00579)
Key     From     To       Description   Tag   Condition   FTGroup
DNA_BIND     573     592       H-T-H motif        
REGION     379     449       Sigma-70 factor domain-2        
REGION     458     534       Sigma-70 factor domain-3        
REGION     547     600       Sigma-70 factor domain-4        
MOTIF     403     406       Interaction with polymerase core subunit RpoC     D-[LI]-[IVLF]-[QE]  

Additional information [?]

Size range 310-900 amino acids
Related rules None
Fusion None
Comments RpoD/SigA is the major sigma factor of most bacteria. Sequences, domains and nomenclature are highly variable. It is called RpoD in E.coli, and SigA in Bacillota and Actinomycetota.