HAMAP rule MF_00963
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00963 |
| Accession | MF_00963 |
| Dates | 31-MAY-2013 (Created)
1-JUN-2023 (Last updated, Version 21) |
| Name | Sigma70_RpoD_SigA |
| Scope(s) |
Bacteria |
| Template(s) | P00579 (RPOD_ECOLI); P0A0J0 (SIGA_STAA8); P77994 (SIGA_THEMA); Q72L95 (SIGA_THET2); Q9EZJ8 (SIGA_THEAQ); [ Recover all ] |
| Triggered by |
HAMAP; MF_00963 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Pseudomonadota> or <OC:Spirochaetales> | |
| Identifier | RPOD |
| Protein name | RecName: Full=RNA polymerase sigma factor RpoD; AltName: Full=Sigma-70; |
| Gene name | Name=rpoD; |
| else | |
| Identifier | SIGA |
| Protein name | RecName: Full=RNA polymerase sigma factor SigA; |
| Gene name | Name=sigA; |
| end case | |
Comments
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| FUNCTION | Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. |
| SUBUNIT | Interacts transiently with the RNA polymerase catalytic core. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the sigma-70 factor family. RpoD/SigA subfamily. |
Keywords
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Gene Ontology
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| GO:0003677; Molecular function:DNA binding |
| GO:0016987; Molecular function:sigma factor activity |
| GO:0010468; Biological process:regulation of gene expression |
| GO:0006352; Biological process:DNA-templated transcription initiation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS00715; SIGMA70_1; 1; |
| PROSITE | PS00716; SIGMA70_2; 1; |
| Pfam | PF04546; Sigma70_ner; 0-1; |
| Pfam | PF03979; Sigma70_r1_1; 0-1; |
| Pfam | PF00140; Sigma70_r1_2; 0-1; |
| Pfam | PF04542; Sigma70_r2; 1; |
| Pfam | PF04539; Sigma70_r3; 1; |
| Pfam | PF04545; Sigma70_r4; 1; |
| PRINTS | PR00046; SIGMA70FCT; 1; |
| NCBIfam | TIGR02393; RpoD_Cterm; 1; |
| NCBIfam | TIGR02937; sigma70-ECF; 1; |
| NCBIfam | TIGR02997; Sig70-cyanoRpoD; 0-1; |
Features
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| From: RPOD_ECOLI (P00579) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DNA_BIND | 573 | 592 | /note="H-T-H motif" | |||||||||
| REGION | 379 | 449 | /note="Sigma-70 factor domain-2" | |||||||||
| REGION | 458 | 534 | /note="Sigma-70 factor domain-3" | |||||||||
| REGION | 547 | 600 | /note="Sigma-70 factor domain-4" | |||||||||
| MOTIF | 403 | 406 | /note="Interaction with polymerase core subunit RpoC" | D-[LI]-[IVLF]-[QE] | ||||||||
Additional information
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| Size range | 310-900 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | RpoD/SigA is the major sigma factor of most bacteria. Sequences, domains and nomenclature are highly variable. It is called RpoD in E.coli, and SigA in Bacillota and Actinomycetota. |