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Annotation rule MF_01011
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General rule information [?]

Accession MF_01011
Dates 9-APR-2002 (Created)
19-NOV-2019 (Last updated, Version 31)
Name RNA_methyltr_TrmA
Scope
Bacteria; Proteobacteria
Template P23003 (TRMA_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TRMA
Protein name
RecName: Full=tRNA/tmRNA (uracil-C(5))-methyltransferase;
EC 2.1.1.35;
EC 2.1.1.-;
AltName: Full=tRNA (uracil(54)-C(5))-methyltransferase;
AltName: Full=tRNA(m5U54)-methyltransferase;
Short=RUMT;
AltName: Full=tmRNA (uracil(341)-C(5))-methyltransferase;
Gene name
trmA

Comments [?]

Function Dual-specificity methyltransferase that catalyzes the formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 (m5U341) in tmRNA (transfer-mRNA).
Catalytic activity RHEA:42712: S-adenosyl-L-methionine + uridine(54) in tRNA = 5-methyluridine(54) in tRNA + H(+) + S-adenosyl-L-homocysteine
EC 2.1.1.35
RHEA:43612: S-adenosyl-L-methionine + uridine(341) in tmRNA = 5-methyluridine(341) in tmRNA + H(+) + S-adenosyl-L-homocysteine
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily.

Keywords [?]

Methyltransferase
S-adenosyl-L-methionine
Transferase
tRNA processing

Gene Ontology [?]

GO:0030697; Molecular function: S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity.
GO:0030488; Biological process: tRNA methylation.

Cross-references [?]

Pfam PF05958; tRNA_U5-meth_tr; 1;
PROSITE PS51687; SAM_MT_RNA_M5U; 1;
PS01230; TRMA_1; 1;
PS01231; TRMA_2; 1;
TIGRFAMs TIGR02143; TrmA_only; 1;

Features [?]

From: TRMA_ECOLI (P23003)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     324     324       Nucleophile     C  
ACT_SITE     358     358       Proton acceptor     E  
BINDING     190     190       S-adenosyl-L-methionine     Q  
BINDING     218     218       S-adenosyl-L-methionine; via carbonyl oxygen     Y  
BINDING     223     223       S-adenosyl-L-methionine     [NH]  
BINDING     239     239       S-adenosyl-L-methionine     E  
BINDING     299     299       S-adenosyl-L-methionine     D  

Additional information [?]

Size range 346-377 amino acids
Related rules MF_01010 (RLMD)
Fusion None


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