HAMAP rule MF_01011
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01011 |
| Accession | MF_01011 |
| Dates | 28-FEB-2005 (Created)
03-SEP-2024 (Last updated, Version 32) |
| Name | RNA_methyltr_TrmA |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P23003; [ Recover all ] |
| Triggered by |
HAMAP; MF_01011 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TRMA |
| Protein name | RecName: Full=tRNA/tmRNA (uracil-C(5))-methyltransferase; EC=2.1.1.35; EC=2.1.1.-; AltName: Full=tRNA (uracil(54)-C(5))-methyltransferase; AltName: Full=tRNA(m5U54)-methyltransferase; Short=RUMT; AltName: Full=tmRNA (uracil(341)-C(5))-methyltransferase; |
| Gene name | Name=trmA; |
Comments
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| FUNCTION | Dual-specificity methyltransferase that catalyzes the formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 (m5U341) in tmRNA (transfer-mRNA). |
| CATALYTIC ACTIVITY | Reaction=uridine(54) in tRNA + S-adenosyl-L-methionine = 5- methyluridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:42712, Rhea:RHEA-COMP:10167, Rhea:RHEA-COMP:10193, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65315, ChEBI:CHEBI:74447; EC=2.1.1.35; |
| CATALYTIC ACTIVITY | Reaction=uridine(341) in tmRNA + S-adenosyl-L-methionine = 5- methyluridine(341) in tmRNA + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:43612, Rhea:RHEA-COMP:10630, Rhea:RHEA-COMP:10631, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65315, ChEBI:CHEBI:74447; |
| SIMILARITY | Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily. |
Keywords
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Gene Ontology
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| GO:0030697; Molecular function:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent |
| GO:0030488; Biological process:tRNA methylation |
Cross-references
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| Pfam | PF05958; tRNA_U5-meth_tr; 1; |
| PROSITE | PS51687; SAM_MT_RNA_M5U; 1; |
| PROSITE | PS01230; TRMA_1; 1; |
| PROSITE | PS01231; TRMA_2; 1; |
| NCBIfam | TIGR02143; TrmA_only; 1; |
Features
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| From: TRMA_ECOLI (P23003) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 324 | 324 | /note="Nucleophile" | C | ||||||||
| ACT_SITE | 358 | 358 | /note="Proton acceptor" | E | ||||||||
| BINDING | 190 | 190 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
Q | ||||||||
| BINDING | 218 | 218 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
Y | ||||||||
| BINDING | 223 | 223 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[NH] | ||||||||
| BINDING | 239 | 239 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
E | ||||||||
| BINDING | 299 | 299 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | ||||||||
Additional information
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| Size range | 346-377 amino acids |
| Related rules |
MF_01010 |
| Fusion | Nter: None Cter: None |