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Annotation rule MF_01011 |
Accession | MF_01011 |
Dates | 9-APR-2002 (Created) 19-NOV-2019 (Last updated, Version 31) |
Name | RNA_methyltr_TrmA |
Scope | Bacteria; Proteobacteria |
Template | P23003 (TRMA_ECOLI) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Dual-specificity methyltransferase that catalyzes the formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 (m5U341) in tmRNA (transfer-mRNA). |
Catalytic activity | RHEA:42712: S-adenosyl-L-methionine + uridine(54) in tRNA = 5-methyluridine(54) in tRNA + H(+) + S-adenosyl-L-homocysteine
EC 2.1.1.35 |
RHEA:43612: S-adenosyl-L-methionine + uridine(341) in tmRNA = 5-methyluridine(341) in tmRNA + H(+) + S-adenosyl-L-homocysteine | |
Similarity | Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily. |
Pfam | PF05958; tRNA_U5-meth_tr; 1; |
PROSITE | PS51687; SAM_MT_RNA_M5U; 1; |
PS01230; TRMA_1; 1; | |
PS01231; TRMA_2; 1; | |
TIGRFAMs | TIGR02143; TrmA_only; 1; |
From: TRMA_ECOLI (P23003) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 324 | 324 | Nucleophile | C | ||||||||
ACT_SITE | 358 | 358 | Proton acceptor | E | ||||||||
BINDING | 190 | 190 | S-adenosyl-L-methionine | Q | ||||||||
BINDING | 218 | 218 | S-adenosyl-L-methionine; via carbonyl oxygen | Y | ||||||||
BINDING | 223 | 223 | S-adenosyl-L-methionine | [NH] | ||||||||
BINDING | 239 | 239 | S-adenosyl-L-methionine | E | ||||||||
BINDING | 299 | 299 | S-adenosyl-L-methionine | D |
Size range | 346-377 amino acids |
Related rules | MF_01010 (RLMD) |
Fusion | None |