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HAMAP rule MF_01011

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General rule information [?]

Accession MF_01011
Dates 9-APR-2002 (Created)
13-OCT-2023 (Last updated, Version 36)
Name RNA_methyltr_TrmA
Scope(s) Bacteria
Template(s) P23003 (TRMA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01011 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TRMA
Protein name RecName: Full=tRNA/tmRNA (uracil-C(5))-methyltransferase;
AltName: Full=tRNA (uracil(54)-C(5))-methyltransferase;
AltName: Full=tRNA(m5U54)-methyltransferase;
AltName: Full=tmRNA (uracil(341)-C(5))-methyltransferase;
Gene name Name=trmA;

Comments [?]

FUNCTIONDual-specificity methyltransferase that catalyzes the formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 (m5U341) in tmRNA (transfer-mRNA).
CATALYTIC ACTIVITY Reaction=S-adenosyl-L-methionine + uridine(54) in tRNA = 5- methyluridine(54) in tRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42712, Rhea:RHEA-COMP:10167, Rhea:RHEA-COMP:10193, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65315, ChEBI:CHEBI:74447; EC=;
CATALYTIC ACTIVITY Reaction=S-adenosyl-L-methionine + uridine(341) in tmRNA = 5- methyluridine(341) in tmRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43612, Rhea:RHEA-COMP:10630, Rhea:RHEA-COMP:10631, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65315, ChEBI:CHEBI:74447;
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0030697; Molecular function:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent
GO:0030488; Biological process:tRNA methylation

Cross-references [?]

Pfam PF05958; tRNA_U5-meth_tr; 1;
PROSITE PS01230; TRMA_1; 1;
PROSITE PS01231; TRMA_2; 1;
NCBIfam TIGR02143; TrmA_only; 1;

Features [?]

From: TRMA_ECOLI (P23003)
Key From To Description Tag Condition FTGroup
ACT_SITE 324 324 /note="Nucleophile" C
ACT_SITE 358 358 /note="Proton acceptor" E
BINDING 190 190 /ligand="S-adenosyl-L-methionine"
BINDING 218 218 /ligand="S-adenosyl-L-methionine"
BINDING 223 223 /ligand="S-adenosyl-L-methionine"
BINDING 239 239 /ligand="S-adenosyl-L-methionine"
BINDING 299 299 /ligand="S-adenosyl-L-methionine"

Additional information [?]

Size range 346-377 amino acids
Related rules MF_01010
Fusion Nter: None Cter: None

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