HAMAP rule MF_01027
General rule information
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| Accession | MF_01027 |
| Dates | 17-JUL-2002 (Created) 19-NOV-2022 (Last updated, Version 39) |
| Name | LeuC_type2 |
| Scope | Bacteria
Archaea |
| Templates | P0A6A6 (LEUC_ECOLI); P15717 (LEUC1_SALTY): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. |
| Catalytic activity | RHEA:32287: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
EC 4.2.1.33 |
| Cofactor | [4Fe-4S] cluster Note: Binds 1 [4Fe-4S] cluster per subunit. |
| Pathway | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. |
| Subunit | Heterodimer of LeuC and LeuD. |
| Similarity | Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily. |
Keywords
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Amino-acid biosynthesis
Branched-chain amino acid biosynthesis
Leucine biosynthesis
Lyase
Metal-binding
Iron
Iron-sulfur
4Fe-4S
Branched-chain amino acid biosynthesis
Leucine biosynthesis
Lyase
Metal-binding
Iron
Iron-sulfur
4Fe-4S
Gene Ontology
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GO:0003861; Molecular function: 3-isopropylmalate dehydratase activity.
GO:0009098; Biological process: leucine biosynthetic process.
GO:0009098; Biological process: leucine biosynthetic process.
Cross-references
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| Pfam | PF00330; Aconitase; 1; |
| PRINTS | PR00415; ACONITASE; 1; |
| TIGRFAMs | TIGR01343; hacA_fam; 1; |
| TIGR02086; IPMI_arch; 1; | |
| TIGR02083; LEU2; 1; | |
| PROSITE | PS00450; ACONITASE_1; 1; |
| PS01244; ACONITASE_2; 1; |
Features
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| From: LEUC_AQUAE (O67078) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 299 | 299 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | ||||||||
| BINDING | 364 | 364 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | ||||||||
| BINDING | 367 | 367 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | ||||||||
Additional information
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| Size range | 380-434 amino acids |
| Related rules | MF_01026 (LEUC) |
| Fusion | None |
| Comments | The family member in THET2 is shown to function as a homoaconitase (for lysine biosynthesis), as well as one of the 2 copies found in METJA that catalyzes both the dehydration of (R)-homocitrate and the hydration of cis-homoaconitate for coenzyme B biosynthesis. The other copy in METJA functions both in leucine and isoleucine biosynthesis, since it catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate and between 2-methylmalate and 3-methylmalate. It seems to be impossible to automatically distinguish the large subunit of homoaconitase (HacA) from that of 3-isopropylmalate dehydratase (LeuC). |