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Annotation rule MF_01037
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General rule information [?]

Accession MF_01037
Dates 28-NOV-2002 (Created)
27-FEB-2020 (Last updated, Version 30)
Name TrmFO
Scope
Bacteria
Templates P39815 (TRMFO_BACSU); Q9S449 (TRMFO_MYXXD): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TRMFO
Protein name
RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO;
EC 2.1.1.74;
AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase;
AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase;
Gene name
trmFO

Comments [?]

Function Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.
Catalytic activity RHEA:16873: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NAD(+)
EC 2.1.1.74
RHEA:62372: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NADP(+)
EC 2.1.1.74
Cofactor FAD
Subcellular location Cytoplasm.
Similarity Belongs to the MnmG family. TrmFO subfamily.

Keywords [?]

Cytoplasm
FAD
Flavoprotein
Methyltransferase
NAD
NADP
Transferase
tRNA processing

Gene Ontology [?]

GO:0050660; Molecular function: flavin adenine dinucleotide binding.
GO:0030698; Molecular function: 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity.
GO:0008033; Biological process: tRNA processing.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01134; GIDA; 1;
TIGRFAMs TIGR00137; Gid_trmFO; 1;
PROSITE PS01280; GIDA_1; 1;
PS01281; GIDA_2; 1;

Features [?]

From: TRMFO_BACSU (P39815)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     10     15       FAD     G-x-G-[LIM]-[AS]-G  

Additional information [?]

Size range 364-482 amino acids
Related rules MF_00129 (MNMG)
Fusion None


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