HAMAP rule MF_01037
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01037 |
| Accession | MF_01037 |
| Dates | 28-NOV-2002 (Created)
3-SEP-2024 (Last updated, Version 35) |
| Name | TrmFO |
| Scope(s) |
Bacteria |
| Template(s) | P39815 (TRMFO_BACSU); Q9S449 (TRMFO_MYXXD); [ Recover all ] |
| Triggered by |
HAMAP; MF_01037 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TRMFO |
| Protein name | RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO; EC=2.1.1.74; AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase; AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase; |
| Gene name | Name=trmFO; |
Comments
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| FUNCTION | Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs. |
| CATALYTIC ACTIVITY | Reaction=uridine(54) in tRNA + (6R)-5,10-methylene-5,6,7,8- tetrahydrofolate + NADH + H(+) = 5-methyluridine(54) in tRNA + (6S)- 5,6,7,8-tetrahydrofolate + NAD(+); Xref=Rhea:RHEA:16873, Rhea:RHEA- COMP:10167, Rhea:RHEA-COMP:10193, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636, ChEBI:CHEBI:57453, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74447; EC=2.1.1.74; |
| CATALYTIC ACTIVITY | Reaction=uridine(54) in tRNA + (6R)-5,10-methylene-5,6,7,8- tetrahydrofolate + NADPH + H(+) = 5-methyluridine(54) in tRNA + (6S)- 5,6,7,8-tetrahydrofolate + NADP(+); Xref=Rhea:RHEA:62372, Rhea:RHEA- COMP:10167, Rhea:RHEA-COMP:10193, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74447; EC=2.1.1.74; |
| COFACTOR | Name=FAD; Xref=ChEBI:CHEBI:57692; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the MnmG family. TrmFO subfamily. |
Keywords
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Gene Ontology
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| GO:0050660; Molecular function:flavin adenine dinucleotide binding |
| GO:0047151; Molecular function:tRNA (uracil(54)-C5)-methyltransferase activity, 5,10-methylenetetrahydrofolate-dependent |
| GO:0008033; Biological process:tRNA processing |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF01134; GIDA; 1; |
| NCBIfam | TIGR00137; Gid_trmFO; 1; |
| PROSITE | PS01280; GIDA_1; 1; |
| PROSITE | PS01281; GIDA_2; 1; |
Features
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| From: TRMFO_BACSU (P39815) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 10 | 15 | /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692" |
G-x-G-[LIM]-[AS]-G | ||||||||
Additional information
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| Size range | 364-482 amino acids |
| Related rules |
MF_00129 |
| Fusion | Nter: None Cter: None |